Pulsed-field gel electrophoresis and ribotype profiles of clinical and environmental Vibrio vulnificus isolates

Applied and Environmental Microbiology

Volumn 62, Issue 10, 1996, Pages 3572-3580

  Tamplin, Mark L ^a,c   Jackson, J Keith ^a   Buchrieser, Carmen ^b   Murphree, Rendi L ^a   Portier, Kenneth M ^a   Gangar, Vidhya ^a   Miller, Leslie G ^a   Kaspar, Charles W ^b  

^a UNIVERSITY OF FLORIDA   (United States)

^b UNIVERSITY OF WISCONSIN MADISON   (United States)

^c UNIVERSITY OF FLORIDA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; BACTERIAL FLORA; ENVIRONMENTAL MONITORING; HEALTH HAZARD; PHENOTYPE; PULSED FIELD GEL ELECTROPHORESIS; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; SHELLFISH; SPECIES DIFFERENTIATION; STATISTICAL ANALYSIS; VIBRIO VULNIFICUS;

BACTERIA (MICROORGANISMS); HAWAIIA; VIBRIO VULNIFICUS;

EID: 0029743083     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/aem.62.10.3572-3580.1996     Document Type: Article

References (55)

1. Amako, D., K. Okada, and S. Miake. 1984. Presence of a capsule in Vibrio vulnificus. J. Gen. Microbiol. 130:2741-2743.
2. Amaro, C., E. G. Biosca, B. Fouz, and E. Garay. 1992. Electrophoretic analysis of heterogeneous lipopolysaccharide from various strains of Vibrio vulnificus biotypes 1 and 2 by silver staining and immunoblotting. Curr. Microbiol. 25:99-104.
3. Aznar, R., W. Ludwig, and K. H. Schleifer. 1993. Ribotyping and randomly amplified polymorphic DNA analysis of Vibrio vulnificus biotypes. Syst. Appl. Microbiol. 16:303-309.
4. Biosca, E. G., E. Garay, A. E. Toranzo, and C. Amaro. 1993. Comparison of outer membrane protein profiles of Vibrio vulnificus biotypes 1 and 2. FEMS Microbiol. Lett. 107:217-222.
5. Bohm, H., and H. Karch. 1992. DNA fingerprinting of Escherichia coli O157:H7 by pulsed-field gel electrophoresis. J. Clin. Microbiol. 30:2169-2172.
6. Bonner, J. R., A. S. Coker, C. R. Berryman, and H. M. Pollock. 1983. Spectrum of vibrio infections in a gulf coast community. Ann. Intern. Med. 99:464-469.
7. Brosch, R., C. Buchrieser, and J. Rocourt. 1991. Subtyping of Listeria monocytogenes serovar-4b by use of low-frequency-cleavage restriction endonucleases and pulsed field gel electrophoresis. Res. Microbiol. 142:667-675.
8. Brosch, R., J. Chen, and J. B. Luchansky. 1994. Pulsed-field fingerprinting of listeriae: identification of genomic divisions for Listeria monocytogenes and their correlation with serovar. Appl. Environ. Microbiol. 60:2584-2592.
9. Buchrieser, C., R. Brosch, and J. Rocourt. 1991. Use of pulsed-field gel electrophoresis to compare large DNA-restriction fragments of Listeria monocytogenes strains belonging to sero-groups 1/2 and 3. Int. Syst. Microbiol. 14:297-304.
10. Buchrieser, C., V. V. Gangar, R. L. Murphree, M. L. Tamplin, and C. W. Kaspar. 1995. Multiple Vibrio vulnificus strains in oysters as demonstrated by clamped homogenous electric field gel electrophoresis. Appl. Environ. Microbiol. 61:1163-1168.
11. Carruthers, M. M., and W. J. Kabat. 1981. Vibrio vulnificus (lactose-positive vibrio) and Vibrio parahemolyticus differ in their susceptibility to human serum. Infect. Immun. 32:964-966.
12. Dice, L. R. 1945. Measures of the amount of ecological association between species. Ecology 26:297-302.
13. Food and Nutrition Board Institute of Medicine. 1991. Seafood Safety/ Committee on Evaluation of the Safety of Fishery Products. National Academy Press, Washington, D.C.
14. Gray, L. D., and A. S. Kreger. 1987. Mouse skin damage caused by cytolysin from Vibrio vulnificus and by Vibrio vulnificus infection. J. Infect. Dis. 155: 236-241.
15. Gray, L. D., and A. S. Kreger. 1989. Detection of Vibrio vulnificus cytolysin in V. vulnificus-infected mice. Toxicon 27:459-464.
16. Harris-Young, L., M. L. Tamplin, J. W. Mason, H. L. Aldrich, and J. K. Jackson. 1995. Viability of Vibrio vulnificus in association with hemocytes of the American oyster (Crassostrea virginica). Appl. Environ. Microbiol. 61: 52-57.
17. Hayat, U. K., G. P. Reddy, C. A. Bush, J. A. Johnson, A. C. Wright, and J. G. Morris, Jr. 1993. Capsular types of Vibrio vulnificus: an analysis of strains from clinical and environmental sources. J. Infect. Dis. 168:758-762.
18. Hlady, W. G., R. C. Mullen, and R. S. Hopkins. 1993. Vibrio vulnificus from raw oysters: leading cause of reported deaths from foodborne illness in Florida. J. Fla. Med. Assoc. 80:536-538.
19. Howard, R., B. Brennman, and S. Lieb. 1988. Soft tissue infections in Florida due to marine vibrio bacteria. J. Fla. Med. Assoc. 73:29-34.
20. Johnson, D. E., and F. M. Calia. 1981. Hemolytic reaction of clinical and environmental strains of Vibrio vulnificus. J. Clin. Microbiol. 14:457-459.
21. Kaysner, C. A., C. Abeyta, M. M. Wekell, A. DePaola, R. F. Stott, and J. M. Leitch. 1987. Virulent strains of Vibrio vulnificus isolated from estuaries of the United States west coast. Appl. Environ. Microbiol. 53:1349-1351.
22. Kreger, A., L. DeChalet and H. Shirley. 1981. Interaction of Vibrio vulnificus with human polymorphonuclear leukocytes: association of virulence with resistance to phagocytosis. J. Infect. Dis. 144:244-248.
23. Kreger, A. S., L. D. Gray, and J. Testa. 1984. Protection of mice against Vibrio vulnificus disease by vaccination with surface antigen preparations and anti-surface antigen antisera. Infect. Immun. 45:537-543.
24. Liu, J., M. Scott, A. Prabhakaran, A. Banta, and R. Siebeling. 1992. Antibody-mediated killing of Vibrio vulnificus by normal human serum, abstr. P-4, p. 324. In Abstracts of the 92nd General Meeting of the American Society for Microbiology 1992. American Society for Microbiology, Washington, D.C.
25. Maniatis, T., E. F. Fritsch, and J. Sambrook. 1982. Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
26. Martin, S. J., and R. J. Siebeling. 1989. Serotyping of the human pathogens: Vibrio vulnificus (opaque and translucent phenotypes), Vibrio damsela, and Vibrio hollisae using lipopolysaccharide specific monoclonal antibodies, abstr. C-206, p. 427. In Abstracts of the 89th General Meeting of the American Society for Microbiology 1989. American Society for Microbiology, Washington, D.C.
27. Martin, S. J., and R. J. Siebeling. 1991. Identification of Vibrio vulnificus O serovars with antilipopolysaccharide monoclonal antibody. J. Clin. Microbiol. 29:1684-1688.
28. Miller, J. M., and D. L. Roden. 1991. Preliminary evaluation of Biolog: a carbon source utilization method for bacterial identification. J. Clin. Microbiol. 29:1143-1147.
29. Musher, D. M., D. E. Johnson, F. M. Calia, and A. Goree. 1984. Resistance of Vibrio vulnificus to serum bactericidal and opsonizing factors: relation to virulence in suckling mice and humans. J. Infect. Dis. 150:413-418.
30. National Marine Fisheries Service. 1990. Fisheries statistics of the United States - 1989. Current Fisheries Statistics no. 8900. National Marine Fisheries Service, Washington, D.C.
31. Oliver, J. D., R. A. Warner, and D. R. Cleland. 1982. Distribution and ecology of Vibrio vulnificus and other lactose-fermenting marine vibrios in coastal waters of the southeastern United States. Appl. Environ. Microbiol. 44:1404-1414.
32. Oliver, J. D., R. A. Warner, and D. R. Cleland. 1983. Distribution of Vibrio vulnificus and other lactose-fermenting vibrios in the marine environment. Appl. Environ. Microbiol. 45:985-998.
33. Prevost, G., B. Jaulhac, and Y. Piemont. 1992. DNA fingerprinting by pulsed-field gel electrophoresis is more effective than ribotyping in distinguishing among methicillin-resistant Staphylococcus aureus isolates. J. Clin. Microbiol. 30:967-973.
34. Reddy, G. P., U. Hayat, C. Abeygunawardana, C. Fox, A. C. Wright, D. R. Maneval, Jr., C. A. Bush, and J. G. Morris, Jr. 1992. Purification and determination of the structure of capsular polysaccharide of Vibrio vulnificus MO6-24. J. Bacteriol. 174:2620-2630.
35. Reyes, A. L., G. N. Stelma, J. T. Peeler, C. H. Johnson, and P. L. Spaulding. 1992. Virulence characteristics of clinical and environmental isolates of Vibrio vulnificus. Appl. Environ. Microbiol. 58:2776-2782.
36. Rodrick, G. E., M. A. Hood, and N. J. Blake. 1982. Human vibrio gastroenteritis. Med. Clin. North Am. 66:665-673.
37. Romesburg, H. C. 1984. Cluster analysis for researchers. Lifetime Learning Publications, Belmont, Calif.
38. Schoonmaker, D., T. Heimberger, and G. Birkhead. 1992. Comparison of ribotyping and restriction enzyme analysis using pulsed-field gel electrophoresis for distinguishing Legionella pneumophilia isolates obtained during a nosocomial outbreak. J. Clin. Microbiol. 30:1491-1498.
39. Simonson, J. G., P. Danieu, and R. J. Siebeling. 1994. Distribution of Vibrio vulnificus capsular polysaccharide and lipopolysaccharide serotypes, abstr. Q-223, p. 427. In Abstracts of the 94th General Meeting of the American Society for Microbiology 1994. American Society for Microbiology, Washington, D.C.
40. Simonson, J. G., and R. J. Siebeling. 1986. Rapid serological identification of Vibrio vulnificus by anti-H coagglutination. Appl. Environ. Microbiol. 52: 1299-1304.
41. Simonson, J. G., and L. Spring. 1992. Analysis of Vibrio vulnificus capsular polysaccharide, abstr. P-5, p. 324. In Abstracts of the 92nd General Meeting of the American Society for Microbiology 1992. American Society for Microbiology, Washington, D.C.
42. Simpson, L. M., and J. D. Oliver. 1983. Siderophore production by Vibrio vulnificus. Infect. Immun. 41:644-649.
43. Simpson, L. M., V. K. White, S. F. Zane, and J. D. Oliver. 1987. Correlation between virulence and colony morphology in Vibrio vulnificus. Infect. Immun. 55:269-272.
44. Snipes, K. P., M. A. Wild, M. W. Miller, D. A. Jessup, R. L. Silflow, W. J. Foreyt, and T. E. Carpenter. 1992. Using ribosomal RNA gene restriction patterns in distinguishing isolates of Pasteurella haemolytica from bighorn sheep (Ovis canadensis). J. Wildl. Dis. 28:347-354.
45. Southern, E. M. 1975. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol. 98:503-517.
46. Tamplin, M. L. 1990. The ecology of Vibrio vulnificus in Crassostrea virginica. Shellfish Institute of North America/National Shellfish Institute, Williamsburg, Va.
47. Tamplin, M. L. 1995. The ecology of Vibrio vulnificus, p. 75-86. In Proceedings of the 1994 Vibrio vulnificus Workshop. U.S. Food and Drug Administration, Washington, D.C.
48. 47a. Tamplin, M. L. Unpublished data.
49. Tamplin, M. L., A. L. Martin, A. D. Ruple, D. W. Cook, and C. W. Kaspar. 1990. Enzyme immunoassay for detection of Vibrio vulnificus in oysters, seawater, and sediment. Appl. Environ. Microbiol. 57:1235-1240.
50. Tamplin, M. L., S. Spector, G. E. Rodrick, and H. Friedman. 1985. Vibrio vulnificus resists phagocytosis in the absence of serum opsonins. Infect. Immun. 49:715-718.
51. Tison, D. L., and M. T. Kelly. 1986. Virulence of Vibrio vulnificus strains from marine environments. Appl. Environ. Microbiol. 51:1004-1006.
52. Wright, A. C., and J. G. Morris. 1991. The extracellular cytolysin of Vibrio vulnificus: inactivation and relationship to virulence in mice. Infect. Immun. 59:192-197.
53. Wright, A. C., L. M. Simpson, and J. D. Oliver. 1981. Role of iron in the pathogenesis of Vibrio vulnificus infections. Infect. Immun. 34:503-507.
54. Wright, A. C., L. M. Simpson, J. D. Oliver, and J. G. Morris, Jr. 1990. Phenotypic evaluation of acapsular transposon mutants of Vibrio vulnificus. Infect. Immun. 58:1769-1773.
55. Yoshida, S. I., M. Ogawa, and Y. Mizuguchi. 1985. Relation of capsular material and colony opacity to virulence of Vibrio vulnificus. Infect. Immun. 47:446-451.