Establishing a TaqMan-Based Real-Time PCR Assay for the rapid detection and quantification of the newly emerged duck tembusu virus

Virology Journal

Volumn 8, Issue , 2011, Pages

  Yan, Liping ^a   Yan, Pixi ^a   Zhou, Jiewen ^a   Teng, Qiaoyang ^a   Li, Zejun ^a  

^a INSTITUTE OF PLANT PROTECTION   (China)

Author keywords

[No Author keywords available]

Indexed keywords

VIRUS DNA; VIRUS RNA; DNA; PRIMER DNA;

ARTICLE; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; DIAGNOSTIC TEST ACCURACY STUDY; DNA EXTRACTION; DUCK TEMBUSU VIRUS; NONHUMAN; NUCLEOTIDE SEQUENCE; PLASMID; REAL TIME POLYMERASE CHAIN REACTION; RELIABILITY; REPRODUCIBILITY; RNA EXTRACTION; SENSITIVITY AND SPECIFICITY; VIRUS; VIRUS DETECTION; VIRUS TITRATION; ANIMAL; ANIMAL DISEASE; BIOSYNTHESIS; BIRD DISEASE; CHEMISTRY; CHICKEN; DUCK; EGG; FLAVIVIRUS; FLAVIVIRUS INFECTION; GENETICS; ISOLATION AND PURIFICATION; METHODOLOGY; VIROLOGY;

ANAS; TEMBUSU VIRUS;

ANIMALS; CHICKENS; DNA; DNA PRIMERS; DUCKS; EGGS; FLAVIVIRUS; FLAVIVIRUS INFECTIONS; POULTRY DISEASES; REAL-TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY;

EID: 80053504136     PISSN: None     EISSN: 1743422X     Source Type: Journal    
DOI: 10.1186/1743-422X-8-464     Document Type: Article

References (16)

1. An infectious disease of ducks caused by a newly emerged Tembusu virus strain in mainland China. Yan P, Zhao Y, Zhang X, Xu D, Dai X, Teng Q, Yan L, Zhou J, Ji X, Zhang S, et al. Virology 2011 417 1 8
2. A novel flavivirus causing duck egg drops and death. Teng Q, Pixi Yan, Xu Zhang, Liping Yan, Zejun Li, Chin J Anim Infect Dis 2010 18 6 1 4
3. Duck egg-drop syndrome caused by BYD virus, a new Tembusu-related flavivirus. Su J, Li S, Hu X, Yu X, Wang Y, Liu P, Lu X, Zhang G, Liu D, Li X, et al. PLoS One 2011 6 18106
4. Rapid and real-time detection of Chikungunya virus by reverse transcription loop-mediated isothermal amplification assay. Parida MM, Santhosh SR, Dash PK, Tripathi NK, Lakshmi V, Mamidi N, Shrivastva A, Gupta N, Saxena P, Babu JP, et al. J Clin Microbiol 2007 45 351 357
5. Morphological features of Murray Valley encephalitis virus infection in the central nervous system of Swiss mice. Matthews V, Robertson T, Kendrick T, Abdo M, Papadimitriou J, McMinn P, Int J Exp Pathol 2000 81 31 40 (Pubitemid 30171499)
6. Development of multiplex real-time reverse transcriptase PCR assays for detecting eight medically important flaviviruses in mosquitoes. Chao DY, Davis BS, Chang GJ, J Clin Microbiol 2007 45 584 589
7. Detection of North American eastern and western equine encephalitis viruses by nucleic acid amplification assays. Lambert AJ, Martin DA, Lanciotti RS, J Clin Microbiol 2003 41 379 385 (Pubitemid 36098603)
8. A duplex real-time reverse transcriptase polymerase chain reaction assay for detecting western equine and eastern equine encephalitis viruses. Kang X, Li Y, Liu H, Lin F, Cai X, Sun T, Chang G, Zhu Q, Yang Y, Virol J 2010 7 284
9. Real-time reverse transcription loop-mediated isothermal amplification for rapid detection of West Nile virus. Parida M, Posadas G, Inoue S, Hasebe F, Morita K, J Clin Microbiol 2004 42 257 263 (Pubitemid 38082729)
10. Detection of west Nile and Japanese encephalitis viral genome sequences in cerebrospinal fluid from acute encephalitis cases in Karachi, Pakistan. Igarashi A, Tanaka M, Morita K, Takasu T, Ahmed A, Ahmed A, Akram DS, Waqar MA, Microbiol Immunol 1994 38 827 830 (Pubitemid 24339459)
11. Detection of Australasian Flavivirus encephalitic viruses using rapid fluorogenic TaqMan RT-PCR assays. Pyke AT, Smith IL, van den Hurk AF, Northill JA, Chuan TF, Westacott AJ, Smith GA, J Virol Methods 2004 117 161 167 (Pubitemid 38369329)
12. Accurate and objective copy number profiling using real-time quantitative PCR. D'Haene B, Vandesompele J, Hellemans J, Methods 2010 50 262 270
13. Real-time PCR quantitation of hepatitis B virus DNA using automated sample preparation and murine cytomegalovirus internal control. Garson JA, Grant PR, Ayliffe U, Ferns RB, Tedder RS, J Virol Methods 2005 126 207 213 (Pubitemid 40558763)
14. Monitoring the immune response using real-time PCR. Stordeur P, Methods Mol Biol 2009 496 323 338
15. Rapid and real-time detection technologies for emerging viruses of biomedical importance. Parida MM, J Biosci 2008 33 617 628
16. Residual viraemia in subjects with chronic HIV infection and viral load < 50 copies/ml: the impact of highly active antiretroviral therapy. Palmisano L, Giuliano M, Nicastri E, Pirillo MF, Andreotti M, Galluzzo CM, Bucciardini R, Fragola V, Andreoni M, Vella S, Aids 2005 19 1843 1847 (Pubitemid 41546603)