Aeromonas hydrophila motY is essential for polar flagellum function, and requires coordinate expression of motX and Pom proteins

Microbiology

Volumn 157, Issue 10, 2011, Pages 2772-2784

  Molero, Raquel ^a   Wilhelms, Markus ^a   Infanzón, Belén ^a   Tomás, Juan M ^a   Merino, Susana ^a  

^a UNIVERSITY OF BARCELONA   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

SODIUM;

AEROMONAS HYDROPHILA; ARTICLE; BACTERIAL FLAGELLUM; BACTERIAL GENE; BACTERIAL GENOME; BACTERIUM MUTANT; ELECTROCHEMICAL ANALYSIS; GENE EXPRESSION; GENOME ANALYSIS; MOTX GENE; MOTY GENE; NONHUMAN; NUCLEOTIDE SEQUENCE; PLASMID; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN INTERACTION; PROTON MOTIVE FORCE; PSEUDOMONAS AERUGINOSA; VIBRIO PARAHAEMOLYTICUS; WILD TYPE;

AEROMONAS HYDROPHILA; AMINO ACID SEQUENCE; BACTERIAL OUTER MEMBRANE PROTEINS; BACTERIAL PROTEINS; BASE SEQUENCE; FLAGELLA; GENE EXPRESSION REGULATION, BACTERIAL; MEMBRANE PROTEINS; MOLECULAR SEQUENCE DATA; PROTEIN BINDING; SEQUENCE ALIGNMENT;

AEROMONAS HYDROPHILA; PSEUDOMONAS AERUGINOSA; VIBRIO PARAHAEMOLYTICUS;

EID: 80053455392     PISSN: 13500872     EISSN: None     Source Type: Journal    
DOI: 10.1099/mic.0.049544-0     Document Type: Article

References (44)

1. Allen, L. N. & Hanson, R. S. (1985). Construction of broad-host-range cosmid cloning vectors: identification of genes necessary for growth of Methylobacterium organophilum on methanol. J Bacteriol 161, 955-962
2. Altarriba, M., Merino, S., Gavín, R., Canals, R., Rabaan, A., Shaw, J. G. & Tomás, J. M. (2003). A polar flagella operon (flg) of Aeromonas hydrophila contains genes required for lateral flagella expression. Microb Pathog 34, 249-259
3. Altschul, S. F., Madden, T. L., Schaffer, A. A., Zhang, J., Zhang, Z., Miller, W. & Lipman, D. J. (1997). Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25, 3389-3402
4. Asai, Y., Kojima, S., Kato, H., Nishioka, N., Kawagishi, I. & Homma, M. (1997). Putative channel components for the fast-rotating sodium-driven flagellar motor of a marine bacterium. J Bacteriol 179, 5104-5110
5. +-driven flagellar motors. J Mol Biol 327, 453-463
6. Bateman, A., Birney, E., Cerruti, L., Durbin, R., Etwiller, L., Eddy, S. R., Griffiths-Jones, S., Howe, K. L., Marshall, M. & Sonnhammer, E. L. L. (2002). The Pfam protein families database. Nucleic Acids Res 30, 276-280
7. Berg, H. C. (2003). The rotary motor of bacterial flagella. Annu Rev Biochem 72, 19-54
8. Blair, D. F. (2003). Flagellar movement driven by proton translocation. FEBS Lett 545, 86-95
9. Blair, D. F. & Berg, H. C. (1990). The MotA protein of E. coli is a proton-conducting component of the flagellar motor. Cell 60, 439-449
10. Canals, R., Ramirez, S., Vilches, S., Horsburgh, G., Shaw, J. G., Tomás, J. M. & Merino, S. (2006a). Polar flagellum biogenesis in Aeromonas hydrophila. J Bacteriol 188, 542-555
11. Canals, R., Altarriba, M., Vilches, S., Horsburgh, G., Shaw, J. G., Tomás, J. M. & Merino, S. (2006b). Analysis of the lateral flagellar gene system of Aeromonas hydrophila AH-3. J Bacteriol 188, 852-862
12. De Mot, R. & Vanderleyden, J. (1994). The C-terminal sequence conservation between OmpA-related outer membrane proteins and MotB suggests a common function in both Gram-positive and Gramnegative bacteria, possibly in the interaction of these domains with peptidoglycan. Mol Microbiol 12, 333-334
13. Doyle, T. B., Hawkins, A. C. & McCarter, L. L. (2004). The complex flagellar torque generator of Pseudomonas aeruginosa. J Bacteriol 186, 6341-6350
14. BAD promoter. J Bacteriol 177, 4121-4130
15. Hanahan, D. (1983). Studies on transformation of Escherichia coli with plasmids. J Mol Biol 166, 557-580
16. Ito, M., Hicks, D. B., Henkin, T. M., Guffanti, A. A., Powers, B. D., Zvi, L., Uematsu, K. & Krulwich, T. A. (2004). MotPS is the stator-force generator for motility of alkaliphilic Bacillus, and its homologue is a second functional Mot in Bacillus subtilis. Mol Microbiol 53, 1035-1049
17. Janda, J. M. & Abbott, S. L. (2010). The genus Aeromonas: taxonomy, pathogenicity, and infection. Clin Microbiol Rev 23, 35-73
18. Jimenez, N., Lacasta, A., Vilches, S., Reyes, M., Vazquez, J., Aquillini, E., Merino, S., Regué, M. & Tomás, J. M. (2009). Genetics and proteomics of Aeromonas salmonicida lipopolysaccharide core biosynthesis. J Bacteriol 191, 2228-2236
19. Koerdt, A., Paulick, A., Mock, M., Jost, K. & Thormann, K. M. (2009). MotX and MotY are required for flagellar rotation in Shewanella oneidensis MR-1. J Bacteriol 191, 5085-5093
20. Kojima, S. & Blair, D. F. (2004). Solubilization and purification of the MotA/MotB complex of Escherichia coli. Biochemistry 43, 26-34
21. Kojima, S., Shinohara, A., Terashima, H., Yakushi, T., Sakuma, M., Homma, M., Namba, K. & Imada, K. (2008). Insights into the stator assembly of the Vibrio flagellar motor from the crystal structure of MotY. Proc Natl Acad Sci U S A 105, 7696-7701
22. MacNab, R. (1996). Flagella and motility. In Escherichia coli and Salmonella, pp. 123-145. Edited by F. C. Neidhardt. Washington, DC: American Society for Microbiology
23. McCarter, L. L. (1994). MotY, a component of the sodium-type flagellar motor. J Bacteriol 176, 4219-4225
24. McCarter, L. L. (2001). Polar flagellar motility of the Vibrionaceae. Microbiol Mol Biol Rev 65, 445-462
25. Merino, S., Camprubí, S. & Tomás, J. M. (1991). The role of lipopolysaccharide in complement-killing of Aeromonas hydrophila strains of serotype O: 34. J Gen Microbiol 137, 1583-1590
26. Okabe, M., Yakushi, T., Asai, Y. & Homma, M. (2001). Cloning and characterization of motX, a Vibrio alginolyticus sodium-driven flagellar motor gene. J Biochem 130, 879-884
27. Okabe, M., Yakushi, T., Kojima, M. & Homma, M. (2002). MotX and MotY, specific components of the sodium-driven flagellar motor, colocalize to the outer membrane in Vibrio alginolyticus. Mol Microbiol 46, 125-134
28. Okabe, M., Yakushi, T. & Homma, M. (2005). Interactions of MotX with MotY and with the PomA/PomB sodium ion channel complex of the Vibrio alginolyticus polar flagellum. J Biol Chem 280, 25659-25664
29. Paulick, A., Koerdt, A., Lassak, J., Huntley, S., Wilms, I., Narberhaus, F. & Thormann, K. M. (2009). Two different stator systems drive a single polar flagellum in Shewanella oneidensis MR-1. Mol Microbiol 71, 836-850
30. Rubirés, X., Saigi, F., Piqué, N., Climent, N., Merino, S., Albertí, S., Tomás, J. M. & Regué, M. (1997). A gene (wbbL) from Serratia marcescens N28b (O4) complements the rfb-50 mutation of Escherichia coli K-12 derivatives. J Bacteriol 179, 7581-7586
31. Sambrook, J., Fritsch, E. F. & Maniatis, T. (1989). Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory
32. Sanger, F., Nicklen, S. & Coulson, A. R. (1977). DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A 74, 5463-5467
33. T: jack of all trades. J Bacteriol 188, 8272-8282
34. Stewart, B. J. & McCarter, L. L. (2003). Lateral flagellar gene system of Vibrio parahaemolyticus. J Bacteriol 185, 4508-4518
35. Stolz, B. & Berg, H. C. (1991). Evidence for interactions between MotA and MotB, torque-generating elements of the flagellar motor of Escherichia coli. J Bacteriol 173, 7033-7037
36. Terashima, H., Fukuoka, H., Yakushi, T., Kojima, S. & Homma, M. (2006). The Vibrio motor proteins, MotX and MotY, are associated with the basal body of Na-driven flagella and required for stator formation. Mol Microbiol 62, 1170-1180
37. Toutain, C. M., Zegans, M. E. & O'Toole, G. A. (2005). Evidence for two flagellar stators and their role in the motility of Pseudomonas aeruginosa. J Bacteriol 187, 771-777
38. Wilhelms, M., Vilches, S., Molero, R., Shaw, J. G., Tomás, J. M. & Merino, S. (2009). Two redundant sodium-driven stator motor proteins are involved in Aeromonas hydrophila polar flagellum rotation. J Bacteriol 191, 2206-2217
39. Yagasaki, J., Okabe, M., Kurebayashi, R., Yakushi, T. & Homma, M. (2006). Roles of the intramolecular disulfide bridge in MotX and MotY, the specific proteins for sodium-driven motors in Vibrio spp. J Bacteriol 188, 5308-5314
40. +-driven polar flagellum of Vibrio alginolyticus. J Bacteriol 186, 5281-5291
41. +-driven flagellar motor of Vibrio. Biochim Biophys Acta 1505, 82-93
42. Yu, H. B., Rao, P. S., Lee, H. C., Vilches, S., Merino, S., Tomas, J. M. & Leung, K. Y. (2004). A type III secretion system is required for Aeromonas hydrophila AH-1 pathogenesis. Infect Immun 72, 1248-1256
43. Zhou, J. D., Fazzio, R. T. & Blair, D. F. (1995). Membrane topology of the MotA protein of Escherichia coli. J Mol Biol 251, 237-242
44. Zhou, J., Lloyd, S. A. & Blair, D. F. (1998). Electrostatic interactions between rotor and stator in the bacterial flagellar motor. Proc Natl Acad Sci U S A 95, 6436-6441