A rapid and cost-effective method for sequencing pooled cDNA clones by using a combination of transposon insertion and gateway technology

BioTechniques

Volumn 51, Issue 3, 2011, Pages 195-197

  Morozumi, Takeya ^a,b   Toki, Daisuke ^a,b   Eguchi Ogawa, Tomoko ^a,c   Uenishi, Hirohide ^a,c  

^a Forestry and Fisheries ^*

^b NATIONAL VETERINARY ASSAY LABORATORY   (Japan)

^c NATIONAL INSTITUTE OF AGROBIOLOGICAL SCIENCES   (Japan)

Author keywords

Automation; cDNA sequencing; Clone pooling; Transposon shotgun sequencing

Indexed keywords

ARTICLE; COST EFFECTIVENESS ANALYSIS; DNA MICROARRAY; DNA SEQUENCE; MOLECULAR CLONING; TRANSPOSON;

ANIMALS; COST-BENEFIT ANALYSIS; DNA TRANSPOSABLE ELEMENTS; DNA, COMPLEMENTARY; GENE LIBRARY; HIGH-THROUGHPUT NUCLEOTIDE SEQUENCING; MUTAGENESIS, INSERTIONAL; SWINE;

EID: 80053450014     PISSN: 07366205     EISSN: 19409818     Source Type: Journal    
DOI: 10.2144/000113737     Document Type: Article

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