Recognition of immunogold markers in electron micrographs

Journal of Structural Biology

Volumn 176, Issue 2, 2011, Pages 151-158

  Wang, Ruixuan ^a   Pokhariya, Himanshu ^a   McKenna, Stephen J ^a   Lucocq, John ^a  

^a UNIVERSITY OF DUNDEE   (United Kingdom)

Author keywords

Immunoelectron microscopy; Immunogold markers; Spot

Indexed keywords

COLLOIDAL GOLD;

ARTICLE; CELL ORGANELLE; IMMUNOGOLD ELECTRON MICROSCOPY; LYSOSOME; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; RECOGNITION; TRANSMISSION ELECTRON MICROSCOPY;

ALGORITHMS; BIOLOGICAL MARKERS; CRYOULTRAMICROTOMY; GOLGI APPARATUS; IMAGE PROCESSING, COMPUTER-ASSISTED; IMMUNOHISTOCHEMISTRY; LYSOSOMES; MICROSCOPY, IMMUNOELECTRON; REPRODUCIBILITY OF RESULTS;

EID: 80053386627     PISSN: 10478477     EISSN: 10958657     Source Type: Journal    
DOI: 10.1016/j.jsb.2011.07.005     Document Type: Article

References (15)

1. Brandt S., Heikkonen J., Engelhardt P. Multiphase method for automatic alignment of transmission electron microscope images using markers. Journal of Structural Biology 2001, 133:10-22.
2. Harris, C., Stephens, M., 1988. A combined corner and edge detector, In: Alvey Vision Conference, 147-152.
3. Kong, J., Dimitrov, M., Yang, Y., Liyanage, J., Cao, L., Staples, J., Mantor, M., Zhou, H., 2010. Accelerating MATLAB image processing toolbox functions on GPUs, In Proceedings of the 3rd Workshop on General-Purpose Computation on Graphics Processing Units (GPGPU '10') ACM, New York, 75-85.
4. Lebonvallet S., Mennesson T., Bonnet N., Girod S., Plotkowski C., Hinnrasky J., Puchelle E. Semi-automatic quantitation of dense markers in cytochemistry. Histochemistry and Cell Biology 1991, 96:245-250.
5. Lindeberg T. Scale-space theory: a basic tool for analyzing structures at different scales. Journal of Applied Statistics 1994, 21(2):225-270.
6. Lowe D.G. Distinctive image features from scale-invariant keypoints. International Journal of Computer Vision 2004, 60:91-110.
7. Lucocq J.M. Quantitation of gold labelling and antigens in immunolabelled ultrathin sections. Journal of Anatomy 1994, 184:1-13.
8. Lucocq J. Quantification of structures and gold labeling in transmission electron microscopy. Methods in Cell Biology 2008, 88:59-82.
9. Lucocq J.M., Gawden-Bone C. A stereological approach for estimation of cellular immunogold labeling and its spatial distribution in oriented sections using the rotator. Journal of Histochemistry and Cytochemistry 2009, 57:709-719.
10. Lucocq J.M., Gawden-Bone C. Quantitative assessment of specificity in immunoelectron microscopy. Journal of Histochemistry and Cytochemistry 2010, 58:917-927.
11. Lucocq J.M., Habermann A., Watt S., Backer J.M., Mayhew T.M., Griffiths G.A. A rapid method for assessing the distribution of gold labeling on thin sections. Journal of Histochemistry and Cytochemistry 2004, 52:991-1000.
12. Mayhew T.M. Quantitative immunoelectron microscopy: alternative ways of assessing subcellular patterns of gold labeling. Methods in Molecular Biology 2007, 369:309-329.
13. Mayhew T., Lucocq J., Griffths G. Relative labelling index: a novel stereological approach to test for non-random immunogold labelling of organelles and membranes on transmission electron microscopy thin sections. Journal of Microscopy 2002, 205:153-164.
14. Monteiro-Leal L.H., Troster H., Campanati L., Spring H., Trendelenburg M.F. Gold finder: a computer method for fast automatic double gold labeling detection, counting, and color overlay in electron microscopic images. Journal of Structural Biology 2003, 141:228-239.
15. Webster P., Schwarz H., Griffiths G. Preparation of cells and tissues for immuno EM. Methods in Cell Biology 2008, 88:45-58.