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Volumn 108, Issue 5, 2011, Pages 821-833

Brachypodium distachyon as a new model system for understanding iron homeostasis in grasses: Phylogenetic and expression analysis of Yellow Stripe-Like (YSL) transporters

Author keywords

Brachypodium distachyon; iron homeostasis; nicotianamine; phytosiderophore; Yellow Stripe Like; YS1; YSL; Zea mays

Indexed keywords

CARRIER PROTEIN; IRON; VEGETABLE PROTEIN;

EID: 80053271962     PISSN: 03057364     EISSN: 10958290     Source Type: Journal    
DOI: 10.1093/aob/mcr200     Document Type: Article
Times cited : (53)

References (50)
  • 3
    • 67650438382 scopus 로고    scopus 로고
    • OsYSL18 is a rice iron(III)-deoxymugineic acid transporter specifically expressed in reproductive organs and phloem of lamina joints
    • Aoyama T, Kobayashi T, Takahashi M, et al. 2009. OsYSL18 is a rice iron(III)-deoxymugineic acid transporter specifically expressed in reproductive organs and phloem of lamina joints. Plant Molecular Biology 70: 681-692.
    • (2009) Plant Molecular Biology , vol.70 , pp. 681-692
    • Aoyama, T.1    Kobayashi, T.2    Takahashi, M.3
  • 4
    • 0000354617 scopus 로고    scopus 로고
    • Arabidopsis Genome Initiative: Analysis of the genome sequence of the flowering plant Arabidopsis thaliana
    • Arabidopsis Genome Initiative
    • Arabidopsis Genome Initiative. 2000. Arabidopsis Genome Initiative: analysis of the genome sequence of the flowering plant Arabidopsis thaliana. Nature 408: 796-813.
    • (2000) Nature , vol.408 , pp. 796-813
  • 5
    • 58149307466 scopus 로고    scopus 로고
    • QuantPrime - A flexible tool for reliable high-throughput primer design for quantitative PCR
    • doi:10.1186/1471-2105-9-465
    • Arvidsson S, Kwasniewski M, Riano-Pachon DM, Mueller-Roeber B. 2008. QuantPrime - a flexible tool for reliable high-throughput primer design for quantitative PCR. BMC Bioinformatics 9: 465. doi:10.1186/1471-2105-9-465.
    • (2008) BMC Bioinformatics , vol.9 , pp. 465
    • Arvidsson, S.1    Kwasniewski, M.2    Riano-Pachon, D.M.3    Mueller-Roeber, B.4
  • 6
    • 0003244354 scopus 로고
    • Yellow-stripe phenotype in maize. I. Effects of ys1 locus on uptake and utilization of iron
    • Bell WD, Bogorad L, McIlrath WJ. 1962. Yellow-stripe phenotype in maize. I. Effects of ys1 locus on uptake and utilization of iron. Botanical Gazette 124: 1-8.
    • (1962) Botanical Gazette , vol.124 , pp. 1-8
    • Bell, W.D.1    Bogorad, L.2    McIlrath, W.J.3
  • 9
    • 77956696826 scopus 로고    scopus 로고
    • Successful reproduction requires the function of Arabidopsis YELLOW STRIPE-LIKE1 and YELLOW STRIPE-LIKE3 metal-nicotianamine transporters in both vegetative and reproductive structures
    • Chu H-H, Chiecko J, Punshon T, et al. 2010. Successful reproduction requires the function of Arabidopsis YELLOW STRIPE-LIKE1 and YELLOW STRIPE-LIKE3 metal-nicotianamine transporters in both vegetative and reproductive structures. Plant Physiology 154: 197-210.
    • (2010) Plant Physiology , vol.154 , pp. 197-210
    • Chu, H.-H.1    Chiecko, J.2    Punshon, T.3
  • 10
    • 0035905794 scopus 로고    scopus 로고
    • Maize yellow stripe1 encodes a membrane protein directly involved in Fe(III) uptake
    • DOI 10.1038/35053080
    • Curie C, Panaviene Z, Loulergue C, Dellaporta SL, Briat JF, Walker EL. 2001. Maize yellow stripe1 encodes a membrane protein directly involved in Fe(III) uptake. Nature 409: 346-349. (Pubitemid 32151239)
    • (2001) Nature , vol.409 , Issue.6818 , pp. 346-349
    • Curie, C.1    Panaviene, Z.2    Loulergue, C.3    Dellaporta, S.L.4    Briat, J.-F.5    Walker, E.L.6
  • 11
    • 57749169737 scopus 로고    scopus 로고
    • Metal movement within the plant: Contribution of nicotianamine and yellow stripe 1-like transporters
    • Curie C, Cassin G, Couch D, et al. 2009. Metal movement within the plant: contribution of nicotianamine and yellow stripe 1-like transporters. Annals of Botany 103: 1-11.
    • (2009) Annals of Botany , vol.103 , pp. 1-11
    • Curie, C.1    Cassin, G.2    Couch, D.3
  • 12
    • 3843101432 scopus 로고    scopus 로고
    • Arabidopsis Yellow Stripe-Like2 (YSL2): A metal-regulated gene encoding a plasma membrane transporter of nicotianamine-metal complexes
    • DOI 10.1111/j.1365-313X.2004.02128.x
    • DiDonato Jr.R., Roberts L, Sanderson T, Eisley R, Walker E. 2004. Arabidopsis Yellow Stripe-Like2 (YSL2): a metal-regulated gene encoding a plasma membrane transporter of nicotianamine-metal complexes. The Plant Journal 39: 403-414. (Pubitemid 39040145)
    • (2004) Plant Journal , vol.39 , Issue.3 , pp. 403-414
    • DiDonato Jr., R.J.1    Roberts, L.A.2    Sanderson, T.3    Eisley, R.B.4    Walker, E.L.5
  • 13
    • 0034533234 scopus 로고    scopus 로고
    • Tightly regulated, β-estradiol dose-dependent expression system for yeast
    • Gao CY, Pinkham JL. 2000. Tightly regulated, beta-estradiol dose-dependent expression system for yeast. Biotechniques 29: 1226-1231. (Pubitemid 32002629)
    • (2000) BioTechniques , vol.29 , Issue.6 , pp. 1226-1231
    • Pinkham, J.L.1
  • 14
    • 33845625713 scopus 로고    scopus 로고
    • TcYSL3, a member of the YSL gene family from the hyper-accumulator Thlaspi caerulescens, encodes a nicotianamine-Ni/Fe transporter
    • DOI 10.1111/j.1365-313X.2006.02937.x
    • Gendre D, Czernic P, Conejero G, et al. 2007. TcYSL3, a member of the YSL gene family from the hyper-accumulator Thlaspi caerulescens, encodes a nicotianamine-Ni/Fe transporter. The Plant Journal 49: 1-15. (Pubitemid 44952693)
    • (2007) Plant Journal , vol.49 , Issue.1 , pp. 1-15
    • Gendre, D.1    Czernic, P.2    Conejero, G.3    Pianelli, K.4    Briat, J.-F.5    Lebrun, M.6    Mari, S.7
  • 16
    • 57049155443 scopus 로고    scopus 로고
    • Exploring valid reference genes for gene expression studies in Brachypodium distachyon by real-time PCR
    • doi:10.1186/1471-2229-8-112
    • Hong S-Y, Seo PJ, Yang M-S, Xiang F, Park C-M. 2008. Exploring valid reference genes for gene expression studies in Brachypodium distachyon by real-time PCR. BMC Plant Biology 8: 112. doi:10.1186/1471-2229-8-112.
    • (2008) BMC Plant Biology , vol.8 , pp. 112
    • Hong, S.-Y.1    Seo, P.J.2    Yang, M.-S.3    Xiang, F.4    Park, C.-M.5
  • 17
    • 33847043486 scopus 로고    scopus 로고
    • Construction and characterization of two BAC libraries from Brachypodium distachyon, a new model for grass genomics
    • Huo N, Gu YQ, Lazo GR, et al. 2006. Construction and characterization of two BAC libraries from Brachypodium distachyon, a new model for grass genomics. Genome 49: 1099-1108.
    • (2006) Genome , vol.49 , pp. 1099-1108
    • Huo, N.1    Gu, Y.Q.2    Lazo, G.R.3
  • 18
    • 63649084522 scopus 로고    scopus 로고
    • Rice OsYSL15 is an ironregulated iron(III)-deoxymugineic acid transporter expressed in the roots and is essential for iron uptake in early growth of the seedlings
    • Inoue H, Kobayashi T, Nozoye T, et al. 2009. Rice OsYSL15 is an ironregulated iron(III)-deoxymugineic acid transporter expressed in the roots and is essential for iron uptake in early growth of the seedlings. Journal of Biological Chemistry 284: 3470-3479.
    • (2009) Journal of Biological Chemistry , vol.284 , pp. 3470-3479
    • Inoue, H.1    Kobayashi, T.2    Nozoye, T.3
  • 19
    • 76749150030 scopus 로고    scopus 로고
    • Genome sequencing and analysis of the model grass Brachypodium distachyon
    • International Brachypodium Initiative
    • International Brachypodium Initiative. 2010. Genome sequencing and analysis of the model grass Brachypodium distachyon. Nature 463: 763-768.
    • (2010) Nature , vol.463 , pp. 763-768
  • 20
    • 84907150192 scopus 로고    scopus 로고
    • The map-based sequence of the rice genome
    • International Rice Genome Sequencing Project
    • International Rice Genome Sequencing Project. 2005. The map-based sequence of the rice genome. Nature 436: 793-800.
    • (2005) Nature , vol.436 , pp. 793-800
  • 23
    • 84948327483 scopus 로고
    • Mugineic acid-family phytosiderophores in root-secretions of barley, com and sorghum varieties
    • Kawai S, Takagi S, Sato Y. 1988. Mugineic acid-family phytosiderophores in root-secretions of barley, com and sorghum varieties. Journal of Plant Nutrition 11: 633-642.
    • (1988) Journal of Plant Nutrition , vol.11 , pp. 633-642
    • Kawai, S.1    Takagi, S.2    Sato, Y.3
  • 24
    • 0035103949 scopus 로고    scopus 로고
    • Evolutionary history of the grasses
    • DOI 10.1104/pp.125.3.1198
    • Kellogg EA. 2001. Evolutionary history of the grasses. Plant Physiology 125: 1198-1205. (Pubitemid 32224610)
    • (2001) Plant Physiology , vol.125 , Issue.3 , pp. 1198-1205
    • Kellogg, E.A.1
  • 25
    • 0035040041 scopus 로고    scopus 로고
    • In vivo evidence that Ids3 from Hordeum vulgare encodes a dioxygenase that converts 2′-deoxymugineic acid to mugineic acid in transgenic rice
    • DOI 10.1007/s004250000453
    • Kobayashi T, Nakanishi H, Takahashi M, Kawasaki S, Nishizawa NK, Mori S. 2001. In vivo evidence that Ids3 from Hordeum vulgare encodes a dioxygenase that converts 2?-deoxymugineic acid to mugineic acid in transgenic rice. Planta 212: 864-871. (Pubitemid 32375891)
    • (2001) Planta , vol.212 , Issue.5-6 , pp. 864-871
    • Kobayashi, T.1    Nakanishi, H.2    Takahashi, M.3    Kawasaki, S.4    Nishizawa, N.-K.5    Mori, S.6
  • 26
    • 18444362441 scopus 로고    scopus 로고
    • Expression of iron-acquisition-related genes in iron-deficient rice is co-ordinately induced by partially conserved iron-deficiency-responsive elements
    • DOI 10.1093/jxb/eri131
    • Kobayashi T, Suzuki M, Inoue H, et al. 2005. Expression of iron-acquisition-related genes in iron-deficient rice is co-ordinately induced by partially conserved iron-deficiency-responsive elements. Journal of Experimental Botany 56: 1305-1316. (Pubitemid 40641008)
    • (2005) Journal of Experimental Botany , vol.56 , Issue.415 , pp. 1305-1316
    • Kobayashi, T.1    Suzuki, M.2    Inoue, H.3    Itai, R.N.4    Takahashi, M.5    Nakanishi, H.6    Mori, S.7    Nishizawa, N.K.8
  • 27
    • 3843129548 scopus 로고    scopus 로고
    • OsYSL2 is a rice metal-nicotianamine transporter that is regulated by iron and expressed in the phloem
    • DOI 10.1111/j.1365-313X.2004.02146.x
    • Koike S, Inoue H, Mizuno D, et al. 2004. OsYSL2 is a rice metal-nicotianamine transporter that is regulated by iron and expressed in the phloem. The Plant Journal 39: 415-424. (Pubitemid 39040146)
    • (2004) Plant Journal , vol.39 , Issue.3 , pp. 415-424
    • Koike, S.1    Inoue, H.2    Mizuno, D.3    Takahashi, M.4    Nakanishi, H.5    Mori, S.6    Nishizawa, N.K.7
  • 28
    • 66649092602 scopus 로고    scopus 로고
    • Disruption of OsYSL15 leads to iron inefficiency in rice plants
    • Lee S, Chiecko JC, Kim SA, et al. 2009. Disruption of OsYSL15 leads to iron inefficiency in rice plants. Plant Physiology 150: 786-800.
    • (2009) Plant Physiology , vol.150 , pp. 786-800
    • Lee, S.1    Chiecko, J.C.2    Kim, S.A.3
  • 29
    • 0001431019 scopus 로고
    • Two related biosynthetic pathways for mugineic acids in gramineous plants
    • Ma JF, Nomoto K. 1993. Two related biosynthetic pathways for mugineic acids in gramineous plants. Plant Physiology 102: 373-378.
    • (1993) Plant Physiology , vol.102 , pp. 373-378
    • Ma, J.F.1    Nomoto, K.2
  • 30
    • 0344153956 scopus 로고    scopus 로고
    • Characterization of phytosiderophore secretion under Fe deficiency stress in Festuca rubra
    • DOI 10.1023/A:1026285813248
    • Ma JF, Ueno H, Ueno D, Rombola AD, Iwashita T. 2003. Characterization of phytosiderophore secretion under Fe deficiency stress in Festuca rubra. Plant and Soil 256: 131-137. (Pubitemid 37435505)
    • (2003) Plant and Soil , vol.256 , Issue.1 , pp. 131-137
    • Ma, J.F.1    Ueno, H.2    Ueno, D.3    Rombola, A.D.4    Iwashita, T.5
  • 32
    • 0001127569 scopus 로고
    • Dynamic state of mugineic acid and analogous phytosiderophores in Fe-deficient barley
    • Mori S, Nishizawa N, Kawai S, Sato Y, Takagi S. 1987. Dynamic state of mugineic acid and analogous phytosiderophores in Fe-deficient barley. Journal of Plant Nutrition 10: 1003-1011.
    • (1987) Journal of Plant Nutrition , vol.10 , pp. 1003-1011
    • Mori, S.1    Nishizawa, N.2    Kawai, S.3    Sato, Y.4    Takagi, S.5
  • 34
    • 0033675662 scopus 로고    scopus 로고
    • Two dioxygenase genes, Ids3 and Ids2, from Hordeum vulgare are involved in the biosynthesis of mugineic acid family phytosiderophores
    • Nakanishi H, Yamaguchi H, Sasakuma T, Nishizawa NK, Mori S. 2000. Two dioxygenase genes, Ids3 and Ids2, from Hordeum vulgare are involved in the biosynthesis of mugineic acid family phytosiderophores. Plant Molecular Biology 44: 199-207.
    • (2000) Plant Molecular Biology , vol.44 , pp. 199-207
    • Nakanishi, H.1    Yamaguchi, H.2    Sasakuma, T.3    Nishizawa, N.K.4    Mori, S.5
  • 35
    • 79953132500 scopus 로고    scopus 로고
    • Phytosiderophore efflux transporters are crucial for iron acquisition in graminaceous plants
    • Nozoye T, Nagasaka S, Kobayashi T, et al. 2011. Phytosiderophore efflux transporters are crucial for iron acquisition in graminaceous plants. Journal of Biological Chemistry 286: 5446-5454.
    • (2011) Journal of Biological Chemistry , vol.286 , pp. 5446-5454
    • Nozoye, T.1    Nagasaka, S.2    Kobayashi, T.3
  • 36
    • 2442641824 scopus 로고    scopus 로고
    • Yellow stripe1. Expanded roles for the maize iron-phytosiderophore transporter
    • DOI 10.1104/pp.103.037572
    • Roberts LA, Pierson AJ, Panaviene Z, Walker EL. 2004. Yellow stripe1. Expanded roles for the maize iron-phytosiderophore transporter. Plant Physiology 135: 112-120. (Pubitemid 38653658)
    • (2004) Plant Physiology , vol.135 , Issue.1 , pp. 112-120
    • Roberts, L.A.1    Pierson, A.J.2    Panaviene, Z.3    Walker, E.L.4
  • 37
    • 0002286083 scopus 로고
    • Evidence for a specific uptake system for iron phytosiderophores in roots of grasses
    • Romheld V, Marchner H. 1986. Evidence for a specific uptake system for iron phytosiderophores in roots of grasses. Plant Physiology 80: 175-180.
    • (1986) Plant Physiology , vol.80 , pp. 175-180
    • Romheld, V.1    Marchner, H.2
  • 38
    • 0033990048 scopus 로고    scopus 로고
    • Primer3 on the WWW for general users and for biologist programmers
    • Rozen S, Skaletsky HJ. 2000. Primer3 on the WWW for general users and for biologist programmers. Methods in Molecular Biology 132: 365-386.
    • (2000) Methods in Molecular Biology , vol.132 , pp. 365-386
    • Rozen, S.1    Skaletsky, H.J.2
  • 39
    • 1542305429 scopus 로고    scopus 로고
    • ZmYS1 Functions as a Proton-coupled Symporter for Phytosiderophore- and Nicotianamine-chelated Metals
    • DOI 10.1074/jbc.M311799200
    • Schaaf G, Ludewig U, Erenoglu BE, Mori S, Kitahara T, Wiren NV. 2004. ZmYS1 functions as a proton-coupled symporter for phytosiderophoreand nicotianamine-chelated metals. Journal of Biological Chemistry 279: 9091-9096. (Pubitemid 38295973)
    • (2004) Journal of Biological Chemistry , vol.279 , Issue.10 , pp. 9091-9096
    • Schaaf, G.1    Ludewig, U.2    Erenoglu, B.E.3    Mori, S.4    Kitahara, T.5    Von Wiren, N.6
  • 40
    • 84948277019 scopus 로고
    • Physiological aspect of mugineic acid, a possible phytosiderophore of graminaceous plants
    • Tagaki S, Nomoto K, Takemoto T. 1984. Physiological aspect of mugineic acid, a possible phytosiderophore of graminaceous plants. Journal of Plant Nutrition 7: 469-477.
    • (1984) Journal of Plant Nutrition , vol.7 , pp. 469-477
    • Tagaki, S.1    Nomoto, K.2    Takemoto, T.3
  • 41
    • 79957613599 scopus 로고    scopus 로고
    • MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods
    • doi:10.1093/molbev/msr121
    • Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. 2011. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular Biology and Evolution, in press. doi:10.1093/molbev/msr121.
    • (2011) Molecular Biology and Evolution
    • Tamura, K.1    Peterson, D.2    Peterson, N.3    Stecher, G.4    Nei, M.5    Kumar, S.6
  • 42
    • 0027968068 scopus 로고
    • CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice
    • Thompson JD, Higgins DG, Gibson TJ. 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research 22: 4673-4680. (Pubitemid 24354800)
    • (1994) Nucleic Acids Research , vol.22 , Issue.22 , pp. 4673-4680
    • Thompson, J.D.1    Higgins, D.G.2    Gibson, T.J.3
  • 43
    • 33947388330 scopus 로고    scopus 로고
    • Identification of two novel phytosiderophores secreted by perennial grasses
    • DOI 10.1111/j.1469-8137.2007.02056.x
    • Ueno D, Rombola A, Iwashita T, Nomoto K, Ma JF. 2007. Identification of two novel phytosiderophores secreted from perennial grasses. New Phytologist 174: 304-310. (Pubitemid 46457401)
    • (2007) New Phytologist , vol.174 , Issue.2 , pp. 304-310
    • Ueno, D.1    Rombola, A.D.2    Iwashita, T.3    Nomoto, K.4    Ma, J.F.5
  • 44
    • 68949151963 scopus 로고    scopus 로고
    • Further characterization of ferric-phytosiderophore transporters ZmYS1 and HvYS1 in maize and barley
    • Ueno D, Yamaji N, Ma JF. 2009. Further characterization of ferric-phytosiderophore transporters ZmYS1 and HvYS1 in maize and barley. Journal of Experimental Botany 60: 3513-3520.
    • (2009) Journal of Experimental Botany , vol.60 , pp. 3513-3520
    • Ueno, D.1    Yamaji, N.2    Ma, J.F.3
  • 45
    • 0037129827 scopus 로고    scopus 로고
    • Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes
    • doi:10.1186/gb-2002-3-7-research0034
    • Vandesompele J, De Preter K, Pattyn F, et al. 2002. Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biology 3: research0034. doi:10.1186/gb-2002-3-7-research0034.
    • (2002) Genome Biology 3: research0034
    • Vandesompele, J.1    De Preter, K.2    Pattyn, F.3
  • 46
    • 41549089817 scopus 로고    scopus 로고
    • High-efficiency Agrobacterium-mediated transformation of Brachypodium distachyon inbred line Bd21-3
    • Vogel J, Hill T. 2008. High-efficiency Agrobacterium-mediated transformation of Brachypodium distachyon inbred line Bd21-3. Plant Cell Reports 27: 471-478.
    • (2008) Plant Cell Reports , vol.27 , pp. 471-478
    • Vogel, J.1    Hill, T.2
  • 47
    • 51649126661 scopus 로고    scopus 로고
    • Time to pump iron: Iron-deficiency-signaling mechanisms of higher plants
    • Walker EL, Connolly EL. 2008. Time to pump iron: iron-deficiency- signaling mechanisms of higher plants. Current Opinion in Plant Biology 11: 530-535.
    • (2008) Current Opinion in Plant Biology , vol.11 , pp. 530-535
    • Walker, E.L.1    Connolly, E.L.2
  • 48
    • 77950104996 scopus 로고    scopus 로고
    • A dynamic gene expression atlas covering the entire life cycle of rice
    • Wang L, Xie W, Chen Y, et al. 2010. A dynamic gene expression atlas covering the entire life cycle of rice. The Plant Journal 61: 752-766.
    • (2010) The Plant Journal , vol.61 , pp. 752-766
    • Wang, L.1    Xie, W.2    Chen, Y.3
  • 49
    • 33747126428 scopus 로고    scopus 로고
    • Mutations in Arabidopsis Yellow Stripe-Like1 and Yellow Stripe-Like3 reveal their roles in metal ion homeostasis and loading of metal ions in seeds
    • DOI 10.1104/pp.106.082586
    • Waters BM, Chu H-H, Didonato RJ, et al. 2006. Mutations in Arabidopsis Yellow Stripe-Like1 and Yellow Stripe-Like3 reveal their roles in metal ion homeostasis and loading of metal ions in seeds. Plant Physiology 141: 1446-1458. (Pubitemid 44223295)
    • (2006) Plant Physiology , vol.141 , Issue.4 , pp. 1446-1458
    • Waters, B.M.1    Chu, H.-H.2    DiDonato, R.J.3    Roberts, L.A.4    Eisley, R.B.5    Lahner, B.6    Salt, D.E.7    Walker, E.L.8
  • 50
    • 0027974805 scopus 로고
    • Iron inefficiency in maize mutant ys1 (Zea mays L. cv Yellow-Stripe) is caused by a defect in uptake of iron phytosiderophores
    • von Wiren N, Mori S, Marschner H, Romheld V. 1994. Iron inefficiency in maize mutant ys1 (Zea mays L. cv Yellow-Stripe) is caused by a defect in uptake of iron phytosiderophores. Plant Physiology 106: 71-77.
    • (1994) Plant Physiology , vol.106 , pp. 71-77
    • Von Wiren, N.1    Mori, S.2    Marschner, H.3    Romheld, V.4


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