C4d immunohistochemical staining is a sensitive method to confirm immunoreactant deposition in formalin-fixed paraffin-embedded tissue in membranous glomerulonephritis

Histology and Histopathology

Volumn 26, Issue 11, 2011, Pages 1391-1397

  Fernando Val Bernal, J ^a   Francisca Garijo, M ^a   Val, Daniel ^a   Rodrigo, Emilio ^a   Arias, Manuel ^a  

^a HOSPITAL UNIVERSITARIO MARQUÉS DE VALDECILLA   (Spain)

Author keywords

C4d; Glomerulonephritis; Immunofluorescence method; Immunoperoxidase methodology; Membranous glomerulonephritis; Paraffin embedded section; Polyclonal antibody

Indexed keywords

COMPLEMENT C4D; COMPLEMENT COMPONENT C4B; FORMALDEHYDE; PARAFFIN; PEPTIDE FRAGMENT;

ADULT; AGED; ARTICLE; FEMALE; HUMAN; IMMUNOHISTOCHEMISTRY; MALE; MEMBRANOUS GLOMERULONEPHRITIS; METHODOLOGY; MIDDLE AGED; SENSITIVITY AND SPECIFICITY; STAINING; TISSUE FIXATION;

ADULT; AGED; COMPLEMENT C4B; FEMALE; FORMALDEHYDE; GLOMERULONEPHRITIS, MEMBRANOUS; HUMANS; IMMUNOHISTOCHEMISTRY; MALE; MIDDLE AGED; PARAFFIN EMBEDDING; PEPTIDE FRAGMENTS; SENSITIVITY AND SPECIFICITY; STAINING AND LABELING; TISSUE FIXATION; YOUNG ADULT;

EID: 80053214190     PISSN: 02133911     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (13)

1. Chowdhury A.R., Ehara T., Higuchi M., Hora K. and Shigematsu H. (2005). Immunohistochemical detection of immunoglobulins and complements in formaldehyde-fixed and paraffin-embedded renal biopsy tissues; an adjunt for diagnosis of glomerulonephritis. Nephrology (Carlton) 10, 298-304.
2. Germuth F.G. and Rodriguez E. (1973). Immunopathology of the renal glomerulus. Immune complex deposit and antibasement membrane disease. Little Brown and Company. Boston. pp 81-91.
3. Kusunoki Y., Itami N., Tochimaru H., Takekoshi Y., Nagasawa S. and Yoshiki T. (1989). Glomerular deposition of C4 cleavage fragment (C4d) and C4-binding protein in idiopathic membranous glomerulonephritis. Nephron 51, 17-19.
4. Lerut E., Kuypers D. and Van Damme B. (2005). C4d deposition in the peritubular capillaries of native renal biopsies. Histopathology 47, 430-432.
5. Li S.J., Liu Z.H., Zen C.H., Wang Q.W., Wang Y. and Li L.S. (2007). Peritubular capillary C4d deposition in lupus nephritis different from antibody-mediated renal rejection. Lupus 14, 875-880.
6. Mölne J., Breimer M.E. and Svalander C.T. (2005). Immunoperoxidase versus immunofluorescence in the assessment of human renal biopsies. Am. J. Kidney Dis. 45, 674-683.
7. Murata K. and Baldwin W.M. III (2009). Mechanisms of complement activation, C4d deposition, and their contribution to the pathogenesis of antibody mediated rejection. Transplant. Rev. 23, 139-150.
8. Nadasdy G.M., Bott C., Cowden D., Pelletier R., Ferguson R. and Nadasdy T. (2005) Comparative study for the detection of peritubular capillary C4d deposition in human renal allograft using different methodologies. Hum. Pathol. 36, 1178-1185.
9. Nasr S.H., Galgano S.J., Markowitz G.S., Stokes M.B. and Dagati V.D. (2006). Immunofluorescence on pronase-digested paraffin sections: a valuable salvage technique for renal biopsies. Kidney Int. 70, 2148-2151.
10. Regele H., Böhmig G.A., Habicht A., Gollowitzer D., Schillinger M., Rockenschaub S., Watschinger B., Kerjaschki D. and Exner M. (2002). Capillary deposition of complement split product C4d in renal allografts is associated with basement membrane injury in peritubular and glomerular capillaries: A contribution of humoral immunity to chronic allograft rejection. J. Am. Soc. Nephrol. 13, 2371-2380.
11. Suzuki T., Horita S., Kadoya K., Mitsuiki K., Aita K., Harada A., Nitta K. and Nagata M. (2007). C4d immunohistochemistry in glomerulonephritis with different antibodies. Clin. Exp. Nephrol. 11, 287-291.
12. Wagrowska-Danilewicz M. and Danilewicz M.(2009). Immunofluorescence on paraffin-embedded sections in evaluation of immune complex deposits in renal biopsy specimens. Pol. J. Pathol. 60, 3-9.
13. Zwirner J., Felber E., Herzog V., Riethmüller G. and Feucht H.E. (1989). Classical pathway of complement activation in normal and diseased human glomeruli. Kidney Int. 36, 1069-1077.