Improving protein crystal quality by the without-oil microbatch method: Crystallization and preliminary X-ray diffraction analysis of glutathione synthetase from Pseudoalteromonas haloplanktis

International Journal of Molecular Sciences

Volumn 12, Issue 9, 2011, Pages 6312-6319

  Merlino, Antonello ^a,b   Krauss, Irene Russo ^a   Albino, Antonella ^a   Pica, Andrea ^a   Vergara, Alessandro ^a,b   Masullo, Mariorosario ^a,c   de Vendittis, Emmanuele ^a   Sica, Filomena ^a,b  

^a UNIVERSITY OF NAPLES FEDERICO II   (Italy)

^b CNR   (Italy)

^c UNIVERSITY OF NAPLES PARTHENOPE   (Italy)

Author keywords

Crystal quality; Glutathione synthetase; Psychrophile; Without oil microbatch; X ray crystallography

Indexed keywords

GLUTATHIONE SYNTHASE; BACTERIAL PROTEIN; RECOMBINANT PROTEIN;

ARTICLE; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; CRYSTAL STRUCTURE; ENZYME PURIFICATION; ESCHERICHIA COLI; EVAPORATION; PROCESS OPTIMIZATION; PROTEIN EXPRESSION; PSEUDOALTEROMONAS; PSEUDOALTEROMONAS HALOPLANKTIS; PSYCHROPHILIC BACTERIUM; QUALITY CONTROL; STRUCTURE ANALYSIS; VALIDATION PROCESS; X RAY DIFFRACTION; CHEMISTRY; CRYSTALLIZATION; ENZYMOLOGY; GENETICS; METABOLISM; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROCEDURES;

ESCHERICHIA COLI; PSEUDOALTEROMONAS HALOPLANKTIS;

BACTERIAL PROTEINS; CRYSTALLIZATION; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GLUTATHIONE SYNTHASE; PSEUDOALTEROMONAS; RECOMBINANT PROTEINS; X-RAY DIFFRACTION;

EID: 80053206933     PISSN: None     EISSN: 14220067     Source Type: Journal    
DOI: 10.3390/ijms12096312     Document Type: Article

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