Screening Pancreatic Oncogenes in Zebrafish Using the Gal4/UAS System

Methods in Cell Biology

Volumn 105, Issue , 2011, Pages 367-381

  Liu, Shu ^a   Leach, Steven D ^a  

^a JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Cloning; Immunofluorescent; Neoplasia; Oncogene; Overexpression; Somatic mutation

Indexed keywords

EID: 80053199978     PISSN: 0091679X     EISSN: None     Source Type: Book Series    
DOI: 10.1016/B978-0-12-381320-6.00015-1     Document Type: Chapter

References (22)

1. SEER Cancer Statistics Review, 1975-2007 2010, National Cancer Institute, Bethesda, MD, http://seer.cancer.gov/csr/1975_2007/, based on November 2009 SEER data submission, posted to the SEER web site, 2010. S.F. Altekruse, C.L. Kosary, M. Krapcho, N. Neyman, R. Aminou, W. Waldron, J. Ruhl, N. Howlader, Z. Tatalovich, H. Cho, A. Mariotto, M.P. Eisner, D.R. Lewis, K. Cronin, H.S. Chen, E.J. Feuer, D.G. Stinchcomb, B.K. Edwards (Eds.).
2. Chen S., Li C., Yuan G., Xie F. Anatomical and histological observation on the pancreas in adult zebrafish. Pancreas 2007, 34:120-125.
3. Davison J.M., Akitake C.M., Goll M.G., Rhee J.M., Gosse N., Baier H., Halpern M.E., Leach S.D., Parsons M.J. Transactivation from Gal4-VP16 transgenic insertions for tissue-specific cell labeling and ablation in zebrafish. Dev. Biol. 2007, 304:811-824.
4. Fisher S., Grice E.A., Vinton R.M., Bessling S.L., Urasaki A., Kawakami K., McCallion A.S. Evaluating the biological relevance of putative enhancers using Tol2 transposon-mediated transgenesis in zebrafish. Nat. Protoc. 2006, 1:1297-1305.
5. Halpern M.E., Rhee J., Goll M.G., Akitake C.M., Parsons M., Leach S.D. Gal4/UAS transgenic tools and their application to zebrafish. Zebrafish 2008, 5:97-110.
6. Hingorani S.R., Petricoin E.F., Maitra A., Rajapakse V., King C., Jacobetz M.A., Ross S., Conrads T.P., Veenstra T.D., Hitt B.A., et al. Preinvasive and invasive ductal pancreatic cancer and its early detection in the mouse. Cancer Cell 2003, 4:437-450.
7. Hruban R.H., Pitman M.B., Klimstra D.S. AFIP Atlas of Tumor Pathology. Tumors of the pancreas 2007, American Registry of Pathology Press, Washington, DC.
8. Jemal A., Siegel R., Xu J., Ward E. Cancer statistics, 2010. CA Cancer J. Clin. 2010, 60:277-300.
9. Jones S., Zhang X., Parsons D.W., Lin J.C., Leary R.J., Angenendt P., Mankoo P., Carter H., Kamiyama H., Jimeno A., et al. Core signaling pathways in human pancreatic cancers revealed by global genomic analyses. Science 2008, 321:1801-1806.
10. Karreth F.A., Tuveson D.A. Modelling oncogenic Ras/Raf signalling in the mouse. Curr. Opin. Genet. Dev. 2009, 19:4-11.
11. Kawaguchi Y., Cooper B., Gannon M., Ray M., MacDonald R.J., Wright C.V. The role of the transcriptional regulator Ptf1a in converting intestinal to pancreatic progenitors. Nat. Genet. 2002, 32:128-134.
12. Kinkel M.D., Prince V.E. On the diabetic menu: zebrafish as a model for pancreas development and function. Bioessays 2009, 31:139-152.
13. Lin J.W., Biankin A.V., Horb M.E., Ghosh B., Prasad N.B., Yee N.S., Pack M.A., Leach S.D. Differential requirement for ptf1a in endocrine and exocrine lineages of developing zebrafish pancreas. Dev. Biol. 2004, 270:474-486.
14. Lund H.Z., Forrest W.W., Harris J.R., Brown E.H. Preliminary embedding in agar-agar. Its value in the processing of multiple separately identified specimens, and in the orientation of small biopsy specimens. Tech. Bull. Regist. Med. Technol. 1961, 31:192-194.
15. Park S.W., Davison J.M., Rhee J., Hruban R.H., Maitra A., Leach S.D. Oncogenic KRAS induces progenitor cell expansion and malignant transformation in zebrafish exocrine pancreas. Gastroenterology 2008, 134:2080-2090.
16. Pisharath H., Parsons M.J. Nitroreductase-mediated cell ablation in transgenic zebrafish embryos. Methods Mol. Biol. 2009, 546:133-143.
17. Tiso N., Moro E., Argenton F. Zebrafish pancreas development. Mol. Cell Endocrinol. 2009, 312:24-30.
18. Urasaki A., Kawakami K. Analysis of genes and genome by the tol2-mediated gene and enhancer trap methods. Methods Mol. Biol. 2009, 546:85-102.
19. Westfield M. The Zebrafish Book. A guide for the Laboratory Use of Zebrafish (Danio rerio). 2007, University of Oregon Press, Eugene.
20. Wilson J.M., Bunte R.M., Carty A.J. Evaluation of rapid cooling and tricaine methanesulfonate (MS222) as methods of euthanasia in zebrafish (Danio rerio). J. Am. Assoc. Lab. Anim. Sci. 2009, 48:785-789.
21. Zecchin E., Mavropoulos A., Devos N., Filippi A., Tiso N., Meyer D., Peers B., Bortolussi M., Argenton F. Evolutionary conserved role of ptf1a in the specification of exocrine pancreatic fates. Dev. Biol. 2004, 268:174-184.
22. Zhan H., Gong Z. Delayed and restricted expression of UAS-regulated GFP gene in early transgenic zebrafish embryos by using the GAL4/UAS system. Mar. Biotechnol. (NY) 2010, 12:1-7.