Microarray and cytokine analyses of field cases of pigs with diarrhoea

Veterinary Microbiology

Volumn 153, Issue 3-4, 2011, Pages 307-314

  Jacobson, M ^a   Andersson, M ^a   Lindberg, R ^a   Fossum, C ^a   Jensen Waern, M ^a  

^a SWEDISH UNIVERSITY OF AGRICULTURAL SCIENCES   (Sweden)

Author keywords

Cytokines; Gene expression; Lawsonia intracellularis; Microarray; PCV2; Swine; Swine dysentery

Indexed keywords

ALPHA1 INTERFERON; GAMMA INTERFERON; INTERLEUKIN 10; INTERLEUKIN 12P40; INTERLEUKIN 1BETA; INTERLEUKIN 6; MESSENGER RNA; SOMATOMEDIN BINDING PROTEIN 3; TUMOR NECROSIS FACTOR ALPHA ANTIBODY;

ADENOMATOSIS; ANIMAL TISSUE; ANTIBODY BLOOD LEVEL; ANTIBODY DETECTION; ARTICLE; CIRCOVIRIDAE INFECTION; CIRCOVIRUS; CONTROLLED STUDY; DESULFOVIBRIONACEAE INFECTION; DIARRHEA; ENTEROPATHY; GENE EXPRESSION; IMMUNE RESPONSE; IMMUNOASSAY; IMMUNOPATHOLOGY; LAWSONIA; MICROARRAY ANALYSIS; NONHUMAN; POLYMERASE CHAIN REACTION; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN MICROARRAY; QUANTITATIVE ANALYSIS; TISSUE SECTION;

ANIMALS; CYTOKINES; DESULFOVIBRIONACEAE INFECTIONS; DIARRHEA; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION; INTESTINES; LAWSONIA BACTERIA; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS; POLYMERASE CHAIN REACTION; PRINCIPAL COMPONENT ANALYSIS; SWINE; SWINE DISEASES;

LAWSONIA INTRACELLULARIS; PORCINE CIRCOVIRUS 2; SUIDAE; SUS;

EID: 80053133359     PISSN: 03781135     EISSN: 18732542     Source Type: Journal    
DOI: 10.1016/j.vetmic.2011.06.003     Document Type: Article

References (40)

1. Andersson M., Berg M., Fossum C., Jensen-Waern M. Development of a microarray for studying porcine cytokine production in blood mononuclear cells and intestinal biopsies. J. Vet. Med. A 2007, 54:161-168.
2. Artursson K., Lindersson M., Varela N., Scheynius A., Alm G.V. Interferon-alpha production and tissue localization of interferon alpha/beta producing cells after intradermal administration of Aujesky's disease virus-infected cells in pigs. Scand. J. Immunol. 1995, 41:121-129.
3. Dahly E.M., Guo Z., Ney D.M. IGF-I augments resection-induced mucosal hyperplasia by altering enterocyte kinetics. Am. J. Physiol. Regul. Integr. Comp. Physiol. 2003, 285:R800-R808.
4. Ersoy B., Ozbilgin K., Kasirga E., Inan S., Coskun S., Tuglu I. Effect of growth hormone on small intestinal homeostasis relation to cellular mediators IGF-1 and IGFBP-3. World J. Gastroenterol. 2009, 21:5418-5424.
5. Fellström C., Pettersson B., Thomson J.R., Gunnarsson A., Persson M., Johansson K.-E. Identification of Serpulina species associated with porcine colitis by biochemical analysis and PCR. J. Clin. Microbiol. 1997, 35:462-467.
6. Firth S.M., Baxter R.M. Cellular actions of the insulin-like growth factor binding proteins. Endocrinol. Rev. 2002, 23:824-854.
7. Fort M., Fernandes L.T., Nofrarias M., Días I., Sibila M., Pujols J., Mateu E., Segalés J. Development of cell-mediated immunity to porcine circovirus type 2 (PCV 2) in ceasarian-derived, colostrum-deprived piglets. Vet. Immunol. Immunopathol. 2009, 129:101-107.
8. Friendship R.M., Corzo C.A., Dewey C.E., Blackwell T. The effect of porcine proliferative enteropathy on the introduction of gilts into recipient herds. J. Swine Health Prod. 2005, 13:139-142.
9. Go Y.-Y., Lee J.-K., Ye J.-Y., Lee J.-B., Park S.-Y., Song C.-S. Experimental reproduction of proliferative enteropathy and the role of IFN-γ in protective immunity against Lawsonia intracellularis in mice. J. Vet. Sci. 2005, 6:357-359.
10. Guedes R.M.C., Gebhart C.J. Comparison of challenge models for porcine proliferative enteropathy. 17th Int. Pig Vet. Soc. Congr., Ames, Iowa, USA 2002, 219.
11. Guedes R.M.C., Gebhart C.J. Onset and duration of fecal shedding, cell-mediated and humoral immune responses in pigs after challenge with a pathogenic isolate or attenuated vaccine strain of Lawsonia intracellularis. Vet. Microbiol. 2003, 91:135-145.
12. Guedes R.M.C., Gebhart C.J. Evidence of cell-mediated immune response and specific local mucosal immunoglobulin (Ig) A production against Lawsonia intracellularis in experimentally infected swine. Can. J. Vet. Res. 2010, 74:97-101.
13. Hasslung Wikström F., Fossum C., Fuxler L., Kruse R., Lövgren T. Cytokine induction by immunostimulatory DNA in porcine PBMC is impaired by a hairpin forming sequence motif from the genome of Porcine Circovirus type 2 (PCV2). Vet. Immunol. Immunopathol. 2011, 139:156-166.
14. Irizarry R.A., Hobbs B., Collin F., Beazer-Barclay Y.D., Antonellis K.J., Scherf U., Speed T.P. Exploration, normalization, and summaries of high density oligonucleotide array probe level data. Biostatistics 2003, 4(2):249-264.
15. Jacobson M., Aspan A., Heldtander Königsson M., Hård af Segerstad C., Wallgren P., Fellström C., Jensen-Waern M., Gunnarsson A. Routine diagnostics of Lawsonia intracellularis performed by PCR, serological and post mortem examination, with special emphasis on sample preparation methods for PCR. Vet. Microbiol. 2004, 102:189-201.
16. Jacobson M., Hård af Segerstad C., Gunnarsson A., Fellström C., de Verdier Klingenberg K., Wallgren P., Jensen-Waern M. Diarrhoea in the growing pig-a comparison of clinical, morphological and microbial findings between animals from good and poor performance herds. Res. Vet. Sci. 2003, 74:163-169.
17. Jacobson M., Wallgren P., Nordengrahn A., Merza M., Emanuelson U. Evaluation of a blocking ELISA for the detection of antibodies to Lawsonia intracellularis in pig sera. Acta Vet. Scand. 2011, 53:23.
18. Jensen T.K., Vigre H., Svensmark B., Bille-Hansen V. Distinction between porcine circovirus type 2 enteritis and porcine proliferative enteropathy caused by Lawsonia intracellularis. J. Comp. Pathol. 2006, 135:176-182.
19. Kim J., Ha Y., Jung K., Choi C., Chae C. Enteritis associated with porcine circovirus 2 in pigs. Can. J. Vet. Res. 2004, 68:219-221.
20. Kruse R., Essén-Gustavsson B., Fossum C., Jensen-Waern M. Blood concentrations of the cytokines IL-1beta, IL-6, IL-10, TNF-alpha and IFN-gamma during experimentally induced swine dysentery. Acta Vet. Scand. 2008, 50:1-7.
21. Lawson G.H.K., Gebhart C.J. Proliferative enteropathy. J. Comp. Pathol. 2000, 122:77-100.
22. Lawson G.H.K., Rowland A.C., Roberts L., Fraser G., McCartney E. Proliferative haemorrhagic enteropathy. Res. Vet. Sci. 1979, 27:46-51.
23. Li C., Wong W.H. Model-based analysis of oligonucleotide arrays: expression index computation and outlier detection. Proc. Natl. Acad. Sci. U.S.A. 2001, 98(1):31-36.
24. Lindecrona R.H., Jensen T.K., Andersen P.H., Möller K. Application of a 5' nuclease assay for detection of Lawsonia intracellularis in fecal samples from pigs. J. Clin. Microbiol. 2002, 40:984-987.
25. -ΔΔCT method. Methods 2001, 25:402-408.
26. Love D.N., Love R.J. Pathology of proliferative haemorrhagic enteropathy in pigs. Vet. Pathol. 1979, 16:41-48.
27. Lund P.K. Molecular basis of intestinal adaptation: the role of the insulin-like growth factor system. Ann. N.Y. Acad. Sci. 1998, 859:18-36.
28. MacIntyre N., Smith D.G.E., Shaw D.J., Thomson J.R., Rhind S. Immunopathogenesis of experimentally induced proliferative enteropathy in pigs. Vet. Pathol. 2003, 40:421-432.
29. McOrist S., MacIntyre N., Stokes C.R., Lawson G.H.K. Immunocytological responses in porcine proliferative enteropathy. Infect. Immun. 1992, 60:4184-4191.
30. Oh Y.-S., Lee J.B., McOrist S. Microarray analysis of differential expression of cell cycle and cell differentiation genes in cells infected with Lawsonia intracellularis. Vet. J. 2010, 184:340-345.
31. Riber U., Boesen H.T., Jakobsen J.T., Nguyen L.T., Jungersen G. Co-incubation with IL-18 potentiates antigen-specific IFN-γ response in a whole-blood stimulation assay for measurement of cell-mediated immune responses in pigs experimentally infected with Lawsonia intracellularis. Vet. Immunol. Immunopathol. 2011, 139:257-263.
32. Rowland A.C., Lawson G.H.K. Intestinal adenomatosis in the pig: immunofluorescent and electron microscopic studies. Res. Vet. Sci. 1974, 17:323-330.
33. Råsbäck T., Fellström C., Gunnarsson A., Aspan A. Comparison of culture and biochemical tests with PCR for detection of Brachyspira hyodysenteriae and Brachyspira pilosicoli. J. Microbiol. Methods 2006, 66:347-353.
34. Smith D.G.E., Lawson G.H.K. Lawsonia intracellularis: getting inside the pathogenesis of proliferative enteropathy. Vet. Microbiol. 2001, 82:331-345.
35. Smith D.G.E., Mitchell S.C., Nash T., Rhind S. Gamma interferon influences intestinal epithelial hyperplasia caused by Lawsonia intracellularis infection in mice. Infect. Immun. 2000, 68:6737-6743.
36. Smyth G.K. Linear models and empirical Bayes methods for assessing differential expression in microarray experiments. Stat. Appl. Genet. Mol. Biol. 2004, 3. (Article 3).
37. Smyth G.K. Limma: Linear Models for Microarray Data. Bioinformatics and Computational Biology Solutions using R and Bioconductor 2005, 397-420. Springer, New York. R. Gentleman, V. Carey, S. Dudoit, R. Irizarry, W. Huber (Eds.).
38. Timmusk S., Wallgren P., Brunborg I.M., Wikström F.H., Allan G., Meehan B., McMenamy M., McNeilly F., Fuxler L., Belák K., Põdersoo D., Saar T., Berg M., Fossum C. Phylogenetic analysis of porcine circovirus type 2 (PCV2) pre- and post-epizootic postweaning multisystemic wasting syndrome (PMWS). Virus Genes 2008, 36:509-520.
39. Thomson J.R., Smith W.J., Fowler V.R., Edwards S.A., Hazzledine M. Non-specific colitis in pigs: defining the condition. 17th Int. Pig Vet. Soc. Congr., Ames, Iowa, USA 2002, 213.
40. Wang Y., Couture O.P., Qu L., Uthe J.J., Bearson S.M.D., Kuhar D., Lunney J.K., Nettleton D., Dekkers J.C.M., Tuggle C.K. Analysis of porcine transcriptional response to Salmonella enterica serovar Cholerasuis suggests novel targets of NFkappaB are activated in the mesenteric lymph node. BMC Genomics 2008, 9:437.