Mutation in the cytoplasmic retrieval signal of porcine epidemic diarrhea virus spike (S) protein is responsible for enhanced fusion activity

Virus Research

Volumn 161, Issue 2, 2011, Pages 188-193

  Shirato, Kazuya ^a   Maejima, Madoka ^a   Matsuyama, Shutoku ^a   Ujike, Makoto ^b   Miyazaki, Ayako ^c   Takeyama, Natsumi ^d   Ikeda, Hidetoshi ^b   Taguchi, Fumihiro ^a,b  

^a NATIONAL INSTITUTE OF INFECTIOUS DISEASES   (Japan)

^b NIPPON VETERINARY AND LIFE SCIENCE UNIVERSITY   (Japan)

^c NATIONAL INSTITUTE OF ANIMAL HEALTH   (Japan)

^d NIPPON INSTITUTE FOR BIOLOGICAL SCIENCE   (Japan)

Author keywords

Coronavirus; Cytoplasmic retrieval signal; Fusion; Porcine epidemic diarrhea virus (PEDV); Spike protein

Indexed keywords

VIRUS SPIKE PROTEIN;

ARTICLE; CELL SURFACE; CONTROLLED STUDY; CYTOPLASM; MUTATIONAL ANALYSIS; NONHUMAN; PORCINE EPIDEMIC DIARRHEA VIRUS; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FUNCTION; VIRUS STRAIN;

AMINO ACID SEQUENCE; ANIMALS; CELL LINE; CORONAVIRUS INFECTIONS; CYTOPLASM; MEMBRANE GLYCOPROTEINS; MOLECULAR SEQUENCE DATA; MUTATION, MISSENSE; PORCINE EPIDEMIC DIARRHEA VIRUS; PROTEIN SORTING SIGNALS; PROTEIN TRANSPORT; SWINE; SWINE DISEASES; VIRAL ENVELOPE PROTEINS; VIRUS INTERNALIZATION;

CORONAVIRUS; MURINAE; PORCINE EPIDEMIC DIARRHEA VIRUS;

EID: 80053131774     PISSN: 01681702     EISSN: 18727492     Source Type: Journal    
DOI: 10.1016/j.virusres.2011.07.019     Document Type: Article

References (25)

1. Bosch B.J., van der Zee R., de Haan C.A., Rottier P.J. The coronavirus spike protein is a class I virus fusion protein: structural and functional characterization of the fusion core complex. J. Virol. 2003, 77(16):8801-8811.
2. Branigan P.J., Liu C., Day N.D., Gutshall L.L., Sarisky R.T., Del Vecchio A.M. Use of a novel cell-based fusion reporter assay to explore the host range of human respiratory syncytial virus F protein. Virol. J. 2005, 2:54.
3. Broer R., Boson B., Spaan W., Cosset F.L., Corver J. Important role for the transmembrane domain of severe acute respiratory syndrome coronavirus spike protein during entry. J. Virol. 2006, 80(3):1302-1310.
4. Chang K.W., Sheng Y., Gombold J.L. Coronavirus-induced membrane fusion requires the cysteine-rich domain in the spike protein. Virology 2000, 269(1):212-224.
5. Corse E., Machamer C.E. Infectious bronchitis virus E protein is targeted to the Golgi complex and directs release of virus-like particles. J. Virol. 2000, 74(9):4319-4326.
6. Fukushi S., Mizutani T., Saijo M., Matsuyama S., Miyajima N., Taguchi F., Itamura S., Kurane I., Morikawa S. Vesicular stomatitis virus pseudotyped with severe acute respiratory syndrome coronavirus spike protein. J. Gen. Virol. 2005, 86(Pt 8):2269-2274.
7. Howard M.W., Travanty E.A., Jeffers S.A., Smith M.K., Wennier S.T., Thackray L.B., Holmes K.V. Aromatic amino acids in the juxtamembrane domain of severe acute respiratory syndrome coronavirus spike glycoprotein are important for receptor-dependent virus entry and cell-cell fusion. J. Virol. 2008, 82(6):2883-2894.
8. Jeetendra E., Ghosh K., Odell D., Li J., Ghosh H.P., Whitt M.A. The membrane-proximal region of vesicular stomatitis virus glycoprotein G ectodomain is critical for fusion and virus infectivity. J. Virol. 2003, 77(23):12807-12818.
9. Kapke P.A., Tung F.Y., Hogue B.G., Brian D.A., Woods R.D., Wesley R. The amino-terminal signal peptide on the porcine transmissible gastroenteritis coronavirus matrix protein is not an absolute requirement for membrane translocation and glycosylation. Virology 1988, 165(2):367-376.
10. Li F., Berardi M., Li W., Farzan M., Dormitzer P.R., Harrison S.C. Conformational states of the severe acute respiratory syndrome coronavirus spike protein ectodomain. J. Virol. 2006, 80(14):6794-6800.
11. Lontok E., Corse E., Machamer C.E. Intracellular targeting signals contribute to localization of coronavirus spike proteins near the virus assembly site. J. Virol. 2004, 78(11):5913-5922.
12. Matsuyama S., Taguchi F. Communication between S1N330 and a region in S2 of murine coronavirus spike protein is important for virus entry into cells expressing CEACAM1b receptor. Virology 2002, 295(1):160-171.
13. Matsuyama S., Taguchi F. Two-step conformational changes in a coronavirus envelope glycoprotein mediated by receptor binding and proteolysis. J. Virol. 2009, 83(21):11133-11141.
14. McBride C.E., Li J., Machamer C.E. The cytoplasmic tail of the severe acute respiratory syndrome coronavirus spike protein contains a novel endoplasmic reticulum retrieval signal that binds COPI and promotes interaction with membrane protein. J. Virol. 2007, 81(5):2418-2428.
15. Pensaert M.B., de Bouck P. A new coronavirus-like particle associated with diarrhea in swine. Arch. Virol. 1978, 58(3):243-247.
16. Petit C.M., Melancon J.M., Chouljenko V.N., Colgrove R., Farzan M., Knipe D.M., Kousoulas K.G. Genetic analysis of the SARS-coronavirus spike glycoprotein functional domains involved in cell-surface expression and cell-to-cell fusion. Virology 2005, 341(2):215-230.
17. Sainz B., Rausch J.M., Gallaher W.R., Garry R.F., Wimley W.C. The aromatic domain of the coronavirus class I viral fusion protein induces membrane permeabilization: putative role during viral entry. Biochemistry 2005, 44(3):947-958.
18. Schwegmann-Wessels C., Al-Falah M., Escors D., Wang Z., Zimmer G., Deng H., Enjuanes L., Naim H.Y., Herrler G. A novel sorting signal for intracellular localization is present in the S protein of a porcine coronavirus but absent from severe acute respiratory syndrome-associated coronavirus. J. Biol. Chem. 2004, 279(42):43661-43666.
19. Shirato K., Maejima M., Hirai A., Ami Y., Takeyama N., Tsuchiya K., Kusanagi K., Nunoya T., Taguchi F. Enhanced cell fusion activity in porcine epidemic diarrhea virus adapted to suckling mice. Arch. Virol. 2010, 155(12):1989-1995.
20. Shirato K., Matsuyama S., Ujike M., Taguchi F. Role of proteases in the release of porcine epidemic diarrhea virus from infected cells. J. Virol. 2011, 85(15):7872-7880.
21. Shirogane Y., Takeda M., Iwasaki M., Ishiguro N., Takeuchi H., Nakatsu Y., Tahara M., Kikuta H., Yanagi Y. Efficient multiplication of human metapneumovirus in Vero cells expressing the transmembrane serine protease TMPRSS2. J. Virol. 2008, 82(17):8942-8946.
22. Taguchi F., Shimazaki Y.K. Functional analysis of an epitope in the S2 subunit of the murine coronavirus spike protein: involvement in fusion activity. J. Gen. Virol. 2000, 81(Pt 12):2867-2871.
23. Tripet B., Howard M.W., Jobling M., Holmes R.K., Holmes K.V., Hodges R.S. Structural characterization of the SARS-coronavirus spike S fusion protein core. J. Biol. Chem. 2004, 279(20):20836-20849.
24. Watanabe R., Matsuyama S., Shirato K., Maejima M., Fukushi S., Morikawa S., Taguchi F. Entry from the cell surface of severe acute respiratory syndrome coronavirus with cleaved S protein as revealed by pseudotype virus bearing cleaved S protein. J. Virol. 2008, 82(23):11985-11991.
25. Weisz O.A., Swift A.M., Machamer C.E. Oligomerization of a membrane protein correlates with its retention in the Golgi complex. J. Cell. Biol. 1993, 122(6):1185-1196.