Multiphoton microscopy of ex vivo corneas after collagen cross-linking

Investigative Ophthalmology and Visual Science

Volumn 52, Issue 8, 2011, Pages 5325-5331

  Bueno, Juan M ^a   Gualda, Emilio J ^a   Giakoumaki, Anastasia ^a   Pérez Merino, Pablo ^b   Marcos, Susana ^b   Artal, Pablo ^a  

^a LABORATORIO DE ÓPTICA   (Spain)

^b INSTITUTO DE ÓPTICA   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

COLLAGEN; RIBOFLAVIN; CROSS LINKING REAGENT; PHOTOSENSITIZING AGENT;

ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; CORNEA STROMA; CORNEA THICKNESS; COW; ENUCLEATION; EX VIVO STUDY; FLUORESCENCE MICROSCOPY; MICROSCOPY; MULTIPHOTON MICROSCOPY; NONHUMAN; PRIORITY JOURNAL; PROTEIN CROSS LINKING; SECOND HARMONIC GENERATION MICROSCOPY; SWINE; ULTRAVIOLET A RADIATION; ANIMAL; CATTLE; DRUG EFFECT; METABOLISM; PATHOLOGY; PHOTOCHEMOTHERAPY; ULTRAVIOLET RADIATION;

ANIMALS; CATTLE; COLLAGEN; CORNEAL STROMA; CROSS-LINKING REAGENTS; MICROSCOPY, FLUORESCENCE, MULTIPHOTON; PHOTOCHEMOTHERAPY; PHOTOSENSITIZING AGENTS; RIBOFLAVIN; SWINE; ULTRAVIOLET RAYS;

EID: 80052955708     PISSN: 01460404     EISSN: 15525783     Source Type: Journal    
DOI: 10.1167/iovs.11-7184     Document Type: Article

References (42)

1. Colin J, Velou S. Current surgical options for keratoconus. J Cataract Refract Surg. 2003;29:379-386.
2. Radner W, Zehetmayer M, Skorpik C, Mallinger R. Altered organization of collagen in the apex of keratoconus corneas. Ophthalmic Res. 1998;30:327-332.
3. Meek KM, Tuft SJ, Huang Y, et al. Changes in collagen orientation and distribution in keratoconus corneas. Invest Ophthalmol Vis Sci. 2005;46:1948-1956.
4. Hayes S, Boote C, Tuft SJ, Quantock AJ, Meek KM. A study of corneal thickness, shape and collagen organisation in keratoconus using videokeratography and X-ray scattering techniques. Exp Eye Res. 2007;84:423-434.
5. Gefen A, Shalom R, Elad D, Mandel Y. Biomechanical analysis of the keratoconic cornea. J Mech Behav Biomed Mater. 2009;2: 224-236.
6. Colin J, Cochener B, Savary G, Malet F. Correcting keratoconus with intracorneal rings. J Cataract Refract Surg. 2000;26:1117-1122.
7. Brierly SC, Izquierdo Jr L, Mannis MJ. Penetrating keratoplasty for keratoconus. Cornea. 2000;19:329-332.
8. Wollensak G, Spoerl E, Seiler T. Riboflavin/ultraviolet-a-induced collagen crosslinking for the treatment of keratoconus. Am J Ophthalmol. 2003;135:620-627.
9. McCall AS, Kraft S, Edelhauser HF, et al. Mechanisms of corneal tissue cross-linking in response to treatment with topical riboflavin and long-wavelength ultraviolet radiation (UVA). Invest Ophthalmol Vis Sci. 2010;51:129-138.
10. Spoerl E, Huhle M, Seiler T. Induction of cross-links in corneal tissue. Exp Eye Res. 1998;66:97-103.
11. Wollensak G, Spoerl E, Seiler T. Stress-strain measurements of human and porcine corneas after riboflavin-ultraviolet-A-induced cross-linking. J Cataract Refract Surg. 2003;29:1780-1785.
12. Wollensak G. Crosslinkining treatment of progressive keratoconus: new hope. Curr Opin Ophthalmol. 2006;17:356-360.
13. Kling S, Remon L, Pérez-Escudero A, Merayo-Lloves J, Marcos S. Corneal biomechanical changes after collagen cross-linking from porcine eye inflation experiments. Invest Ophthalmol Vis Sci. 2010;51:3961-3968.
14. Wollensak G, Spoerl E, Wilsch M, Seiler T. Keratocyte apoptosis after corneal collagen cross-linking using riboflavin/UVA treatment. Cornea. 2004;23:43-49.
15. Spoerl E, Mrochen M, Sliney D, Trokel S, Seiler T. Safety of UVA riboflavin cross-linking of the cornea. Cornea. 2007;26:385-389.
16. Wollensak G, Aurich H, Pham DT, Wirbelbauer C. Hydration behavior of porcine cornea crosslinked with riboflavin and ultraviolet A. J Cataract Refract Surg. 2007;33:516-521.
17. Hafezi F, Kanellopoulos J, Wiltfang R, Seiler T. Corneal collagen crosslinking with riboflavin and ultraviolet A to treat induced keratectasia after laser in situ keratomileusis. J Cataract Refract Surg. 2007;33:2035-2040.
18. Wollensak G. Corneal collagen crosslinking: new horizons. Expert Rev Ophthalmol. 2010;5:201-215.
19. Bottós KM, Dreyfuss JL, Regatieri CV, et al. Immunofluorescence confocal microscopy of porcine corneas following collagen crosslinking treatment with riboflavin and ultraviolet A. J Refract Surg. 2008;24:S715-9.
20. Wollensak G, Wilsch M, Spoerl E, Seiler T. Collagen fiber diameter in the rabbit cornea after collagen crosslinking by riboflavin/UVA. Cornea. 2004;23:503-507.
21. Piston DW, Masters BR, Webb WW. Three-dimensionally resolved NAD(P)H cellular metabolic redox imaging of the in situ cornea with two-photon excitation laser scanning microscopy. J Microsc. 1995;178:20-27.
22. Yeh AT, Nassif N, Zoumi A, Tromberg BJ. Selective corneal imaging using combined second-harmonic generation and two-photon excited fluorescence. Opt Lett. 2002;27:2082-2084.
23. Morishige N, Nishida T, Jester J. Second harmonic generation for visualizing 3-dimensional structure of corneal collagen lamellae. Cornea. 2009;28:46-53.
24. Aptel F, Olivier N, Deniset-Besseau A, et al. Multimodal nonlinear imaging of the human cornea. Invest Ophthalmol Vis Sci. 2010;51:2459-2465.
25. Han M, Giese G, Bille J. Second harmonic generation imaging of collagen fibrils in cornea and sclera. Opt Express. 2005;13:5791-5797.
26. Teng SW, Tan HY, Ju-Li Peng JL, et al. Multiphoton autofluorescence and second-harmonic generation imaging of the ex vivo porcine eye. Invest Ophthalmol Vis Sci. 2006;47:1216-1224.
27. Morishige N, Petroll WM, Nishida T, Kenney MC, Jester JV. Noninvasive corneal stromal collagen imaging using two-photon-generated second-harmonic signals. J Cataract Refract Surg. 2006;32: 1784-1791.
28. Tan HY, Sun Y, Lo W, et al. Multiphoton fluorescence and second harmonic generation imaging of the structural alterations in keratoconus ex vivo. Invest Ophthalmol Vis Sci. 2006;47:5251-5259.
29. Morishige N, Wahlert AJ, Kenney MC, et al. Second-harmonic imaging microscopy of normal human and keratoconus cornea. Invest Ophthalmol Vis Sci. 2007;48:1087-1094.
30. Morishige N, Yamada N, Teranishi S, Chikama T, Nishida T, Takahara A. Detection of subepithelial fibrosis associated with corneal stromal edema by second harmonic generation imaging microscopy. Invest Ophthalmol Vis Sci. 2009;50:3145-3150.
31. Kampik D, Ralla B, Keller S, Hirschberg M, Friedl P, Geerling G. Influence of corneal collagen crosslinking with riboflavin and ultraviolet-A irradiation on excimer laser surgery. Invest Ophthalmol Vis Sci. 2010;51:3929-3934.
32. Gualda EJ, Bueno JM, Artal P. Wavefront optimized non-linear microscopy of ex-vivo human retinas. J Biom Opt. 2010;15:026007.
33. Bueno JM, Gualda EJ, Artal P. Analysis of corneal stroma organization with wavefront optimized nonlinear microscopy. Cornea. 2011;30:692-701.
34. Bueno JM, Gualda EJ, Artal P. Adaptive optics multiphoton microscopy to study ex vivo ocular tissues. J Biom Opt. 2010;15:066004.
35. Steven P, Hovakimyan M, Guthoff RF, Hüttmann G, Stachs O. Imaging corneal crosslinking by autofluorescence 2-photon microscopy, second harmonic generation, and fluorescence lifetime measurements. J Cataract Refract Surg. 2010;36:2150-2159.
36. Kymionis GD, Kounis GA, Portaliou DM, et al. Intraoperative pachymetric measurements during corneal collagen cross-linking with riboflavin and ultraviolet A irradiation. Ophthalmol. 2009;116:2336-2339.
37. Hafezi F, Mrochen M, Iseli HP, Seiler T. Collagen crosslinking with ultraviolet-A and hypoosmolar riboflavin solution in thin corneas. J Cataract Ref Surg. 2009;35:621-624.
38. Meek KM, Fullwood NJ, Cooke PH, et al. Synchrotron x-ray diffraction studies of the cornea with implications for stromal hydration. Biophys J. 1991;60:467-474.
39. Craig AS, Robertson JG, Parry DAD. Preservation of corneal collagen fibril structure using low temperature procedures for electron microscopy. J Ultrastruct Res. 1986;96:172-175.
40. Ahearne M, Yang Y, Then KY, Liu KK. An indentation technique to characterize the mechanical and viscoelastic properties of human and porcine corneas. Ann Biomed Eng. 2007;35:1608-1616.
41. Kling S, Pérez-Merino P, Marcos S. Impact of corneal hydration on corneal biomechanical properties from in vitro inflation tests. J Cataract Refract Surg (submitted. 2010).
42. Vinciguerra P, Albe E, Trazza S, et al. Refractive, topographic, tomographic, and aberrometric analysis of keratoconic eyes undergoing corneal cross-linking. Ophthalmology. 2009;116:369-378.