Analysis of Androgen-Induced Increase in Lipid Accumulation in Prostate Cancer Cells

Methods in Molecular Biology

Volumn 776, Issue , 2011, Pages 371-382

  Sikkeland, Jørgen ^a   Lindstad, Torstein ^a   Saatcioglu, Fahri ^a  

^a UNIVERSITY OF OSLO   (Norway)

Author keywords

confocal microscopy; de novo lipogenesis; neutral lipids; Oil red O; Prostate cancer; spectrophotometric analysis

Indexed keywords

ANDROGEN;

ARTICLE; BIOASSAY; CULTURE TECHNIQUE; DRUG EFFECT; FLUORESCENCE MICROSCOPY; HUMAN; LIPID METABOLISM; MALE; METABOLISM; PATHOLOGY; PROSTATE TUMOR; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; TISSUE FIXATION; TUMOR CELL LINE;

ANDROGENS; BIOLOGICAL ASSAY; CELL CULTURE TECHNIQUES; CELL LINE, TUMOR; HUMANS; LIPID METABOLISM; MALE; MICROSCOPY, FLUORESCENCE; PROSTATIC NEOPLASMS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; TISSUE FIXATION;

EID: 80052951757     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-61779-243-4_22     Document Type: Chapter

References (11)

1. Flavin, R., Peluso, S., Nguyen, P. L., and Loda, M. (2010) Fatty acid synthase as a potential therapeutic target in cancer, Future Oncol 6, 551–562.
2. Menendez, J. A., and Lupu, R. (2007) Fatty acid synthase and the lipogenic phenotype in cancer pathogenesis, Nat Rev Cancer 7, 763–777.
3. Swinnen, J. V., Roskams, T., Joniau, S., Van Poppel, H., Oyen, R., Baert, L., Heyns, W., and Verhoeven, G. (2002) Overexpression of fatty acid synthase is an early and common event in the development of prostate cancer, Int J Cancer 98, 19–22.
4. Rossi, S., Graner, E., Febbo, P., Weinstein, L., Bhattacharya, N., Onody, T., Bubley, G., Balk, S., and Loda, M. (2003) Fatty acid synthase expression defines distinct molecular signatures in prostate cancer, Mol Cancer Res 1, 707–715.
5. Swinnen, J. V., Heemers, H., van de Sande, T., de Schrijver, E., Brusselmans, K., Heyns, W., and Verhoeven, G. (2004) Androgens, lipogenesis and prostate cancer, J Steroid Biochem Mol Biol 92, 273–279.
6. Zadra, G., Priolo, C., Patnaik, A., and Loda, M. (2010) New strategies in prostate cancer: targeting lipogenic pathways and the energy sensor AMPK, Clin Cancer Res 16, 3322–3328.
7. Swinnen, J. V., Esquenet, M., Goossens, K., Heyns, W., and Verhoeven, G. (1997) Androgens stimulate fatty acid synthase in the human prostate cancer cell line LNCaP, Cancer Res 57, 1086–1090.
8. Swinnen, J. V., Van Veldhoven, P. P., Esquenet, M., Heyns, W., and Verhoeven, G. (1996) Androgens markedly stimulate the accumulation of neutral lipids in the human prostatic adenocarcinoma cell line LNCaP, Endocrinology 137, 4468–4474.
9. Swinnen, J. V., Ulrix, W., Heyns, W., and Verhoeven, G. (1997) Coordinate regulation of lipogenic gene expression by androgens: evidence for a cascade mechanism involving sterol regulatory element binding proteins, Proc Natl Acad Sci USA 94, 12975–12980.
10. Heemers, H., Maes, B., Foufelle, F., Heyns, W., Verhoeven, G., and Swinnen, J. V. (2001) Androgens stimulate lipogenic gene expression in prostate cancer cells by activation of the sterol regulatory element-binding protein cleavage activating protein/sterol regulatory element-binding protein pathway, Mol Endocrinol 15, 1817–1828.
11. Heemers, H. V., Verhoeven, G., and Swinnen, J. V. (2006) Androgen activation of the sterol regulatory element-binding protein pathway: Current insights, Mol Endocrinol 20, 2265–2277.