Impact of dynamic culture in the RCCS™ bioreactor on a three-dimensional model of bone matrix formation

Procedia Engineering

Volumn 10, Issue , 2011, Pages 3662-3667

  Mazzoleni, Giovanna ^a   Boukhechba, Florian ^b   Steimberg, Nathalie ^a   Boniotti, Jennifer ^a   Bouler, Jean Michel ^c   Rochet, Nathalie ^d  

^a Division of Internal Medicine   (Italy)

^b Graftys SA   (France)

^c UNIVERSITÉ DE NANTES   (France)

^d UNIVERSITÉ CÔTE D'AZUR   (France)

Author keywords

3D culture; Biphasic calcium phosphate; Bone matrix; Bone tissue engineering; Dynamic culture; Human osteocytes; RCCS bioreactor

Indexed keywords

BIOCONVERSION; BIOREACTORS; BONE; CALCIUM; CELL CULTURE; TISSUE; TISSUE ENGINEERING;

3-D CULTURES; BIPHASIC CALCIUM PHOSPHATES; BONE MATRIX; BONE TISSUE ENGINEERING; OSTEOCYTES;

CALCIUM PHOSPHATE;

EID: 80052942420     PISSN: 18777058     EISSN: None     Source Type: Conference Proceeding    
DOI: 10.1016/j.proeng.2011.04.603     Document Type: Conference Paper

References (16)

1. Mazzoleni G, Steimberg N. 3D culture in microgravity: a realistic alternative to experimental animal use. ALTEX 2010;27:321-4.
2. Pampaloni F, Reynaud EG, Stelzer EH. The third dimension bridges the gap between cell culture and live tissue. Nat. Rev. Mol. Cell Biol., 2007;8:839-45.
3. Mazzoleni G, Di Lorenzo D, Steimberg N. Modelling tissues in 3D: a near future for pharmaco-toxicology anf food research? Genes & Nutrition 2009; 4:13-22.
4. Martin Y, Vermette P. Bioreactors for tissue mass culture: design, characterization, and recent advances. Biomaterials 2005;26:7481-503.
5. Schwarz RP, Goodwin TJ, Wolf DA. Cell culture for three dimensional modeling in rotating-wall vessels: an application of simulated microgravity. J Tissue Cult Methods 1992;14:51-7.
6. Cosmi F, Steimberg N, Dreossi D, Mazzoleni G. Structural analysis of rat bone explants kept in vitro in simulated microgravity conditions. Journal of the Mechanical Behavior of Biomedical Materials 2009;2:164-72.
7. Steimberg N, Boniotti J, Mazzoleni G. Bioengineered osteo-articular systems: 3D culture of bone and cartilage in simulated microgravity. In: Novik E, Maguire T, editors. Methods in Bioengineering: Alternative Technologies to Animal Testing, USA: Artech House publisher; 2010, p. 205-25.
8. Cavagna R, Daculsi G, Bouler JM. Macroporous calcium phosphate ceramic: a prospective study of 106 cases in lumbar spinal fusion. J Long Term Eff Med Implants. 1999;9:403-12.
9. Trojani C, Boukhechba F, Scimeca JC, Vandenbos F, Michiels JF, Daculsi G et al. Ectopic bone formation using an injectable biphasic calcium phosphate/Si-HPMC hydrogel composite loaded with undifferentiated bone marrow stromal cells. Biomaterials 2006;27:3256-64.
10. Balaguer T, Boukhechba F, Clavé A, Bouvet-Gerbettaz S, Trojani C, Michiels JF et al. Biphasic calcium phosphate microparticles for bone formation: benefits of combination with blood clot. Tissue Eng Part A. 2010;16:3495-505.
11. Rochet N, Loubat A, Laugier JP, Hofman P, Bouler JM, Daculsi G et al. Modification of gene expression induced in human osteogenic and osteosarcoma cells by culture on a biphasic calcium phosphate bone substitute. Bone 2003;32:602-10.
12. Mouline CC, Quincey D, Laugier JP, Carle GF, Bouler JM, Rochet N, Scimeca JC. Osteoclastic differentiation of mouse and human monocytes in a plasma clot/biphasic calcium phosphate microparticles composite. Eur Cell Mater. 2010;10:379-92.
13. Boukhechba F, Balaguer T, Michiels JF, Ackermann K, Quincey D, Bouler JM et al. Human primary osteocyte differentiation in a 3D culture system. J Bone Miner Res. 2009;24:1927-35.
14. Bouler JM, LeGeros RZ, Daculsi G. Biphasic calcium phosphates: influence of three synthesis parameters on the HA/betaTCP ratio. J Biomed Mater Res. 2000;51:680-84.
15. Facer SR, Zaharias RS, Andracki ME, Lafoon J, Hunter SK, Schneider GB. Rotary culture enhances pre-osteoblast aggregation and mineralisation. J Dent Res 2005;84:542-47.
16. Lappa M. Organic tissues in rotating bioreactors: fluid-mechanical aspects, dynamic growth models, and morphological evolution. Biotechnol Bioeng 2003; 84:518-32.