Mini-Mu Transposon Mutagenesis of Ethanologenic Zymomonas mobilis

Methods in Molecular Biology

Volumn 765, Issue , 2011, Pages 419-434

  Pappas, Katherine M ^a  

^a UNIVERSITY OF ATHENS   (Greece)

Author keywords

Auxotroph production; Insertional inactivation; Mini Mu; Transposon mutagenesis; Zymomonas mobilis

Indexed keywords

ALPHAPROTEOBACTERIA; PROTEOBACTERIA; ZYMOMONAS MOBILIS;

ARTICLE; BACTERIOPHAGE MU; GENE EXPRESSION REGULATION; GENETICS; TRANSPOSON; VIROLOGY; ZYMOMONAS;

BACTERIOPHAGE MU; DNA TRANSPOSABLE ELEMENTS; MUTAGENESIS, INSERTIONAL; ZYMOMONAS;

EID: 80052940762     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-61779-197-0_25     Document Type: Chapter

References (23)

1. Gunasekaran P., and Raj C. (1999) Ethanol fermentation technology-Zymomonas mobilis. Current Science 77, 56–68.
2. Rogers P.L., Jeon Y.J., Lee K.J., and Lawford H.G. (2007) Zymomonas mobilis for fuel ethanol and higher value products. Adv. Biochem. Eng. Biotechnol. 108, 263–288.
3. Zhang M., Eddy C., Deanda K., Finkelstein M., and Picataggio S. (1995) Metabolic engineering of a pentose metabolism pathway in ethanologenic Zymomonas mobilis. Science 267, 240–243.
4. Deanda K., Zhang M., Eddy C., and Picataggio S. (1996) Development of an ara-binose-fermenting Zymomonas mobilis strain by metabolic pathway engineering. Appl. Environ. Microbiol. 62, 4465–4470.
5. Dien B.S., Cotta M.A., Jeffries T.W. (2003) Bacteria engineered for fuel ethanol production: current status. Appl. Microbiol. Biotechnol. 63, 258–266.
6. Moreau R.A., Powell M.J., Osman S.F., Whitaker B.D., Fett W.F., Roth L., and O’Brien D.J. (1995) Analysis of intact hopanoids and other lipids from the bacterium Zymomonas mobilis by high-performance liquid chromatography. Anal. Biochem. 224, 293–301.
7. Seo J.S., Chong H., Park H.S., Yoon K.O., Jung C., Kim J.J. et al. (2005) The genome sequence of the ethanologenic bacterium Zymomonas mobilis ZM4. Nat. Biotechnol. 23, 63–68.
8. Yang S., Pappas K.M., Hauser L.J., Land M.L., Chen G.L., Hurst G.B. et al. (2009) Improved genome annotation for Zymomonas mobilis. Nat. Biotechnol. 27, 893–894.
9. Kouvelis V.N., Saunders E., Brettin T.S., Bruce D., Detter C., Han C. et al. (2009) Complete genome sequence of the ethanol producer Zymomonas mobilis NCIMB 11163. J. Bacteriol. 191, 7140–7141.
10. Yang S., Tschaplinski T.J., Engle N.L., Carroll S.L., Martin S.L., Davison B.H. et al. (2009) Transcriptomic and metabolomic profiling of Zymomonas mobilis during aerobic and anaerobic fermentations. BMC Genomics 10, 34.
11. Skotnicki M.L., Lee K.J., Tribe D.E., and Rogers P.L. (1982) Genetic alteration of Zymomonas mobilis for ethanol production. In: Hollender A et al (ed) Genetic engineering of microorganisms for chemicals, Plenum Press, NY
12. Typas M.A., and Galani I. (1992) Chemical and UV mutagenesis in Zymomonas mobilis. Genetica 87, 37–45.
13. Sprenger G.A., Typas M.A., and Drainas C. (1993) Genetics and genetic engineering of Zymomonas mobilis. World J. Microbiol. Biotechnol. 9, 17–24.
14. Kalnenieks U., Galinina N., Toma M.M., Pickford J.L., Rutkis R., and Poole R.K. (2006) Respiratory behaviour of a Zymomonas mobilis adhB::kan(r) mutant supports the hypothesis of two alcohol dehydrogenase isoenzymes catalysing opposite reactions. FEBS Lett. 580, 5084–5088.
15. Reznikoff W.S., and Winterberg K.M. (2008) Transposon-based strategies for the identification of essential bacterial genes. Methods Mol. Biol. 416, 13–26.
16. Carey T., Sewell G.M., Osman Y.A., and Ingram L.O. (1987) Expression of the lactose transposon (Tn951) in Zymomonas mobilis. Appl. Environ. Microbiol. 45, 1163–1168.
17. Walker M.J., and Pemberton J.M. (1988) Construction of transposons encoding genes for b-glucosidase, amylase and poly-galac-turonate trans-eliminase from Klebsiella oxy-toca and their expression in a range of gram-negative bacteria. Curr. Microbiol. 17, 69–75.
18. Faelen M., Resibois A., and Toussaint A. (1978) Mini-Mu: an insertion element derived from temperate phage Mu-1. Cold Spring Harbor Symp. Quant. Biol. 43, 1169–1177.
19. Pappas K.M., Galani I., and Typas M.A. (1997) Transposon mutagenesis and strain construction in Zymomonas mobilis. J. Appl. Microbiol. 82, 379–388.
20. Van Gijsengem F., and Toussaint A. (1982) Chromosome transfer and R-prime formation by an RP4::mini-Mu derivative in Escherichia coli, Salmonella typhimurium, Klebsiella pneumoniae and Proteus mirabilis. Plasmid 7, 30–44.
21. Galani I., and Typas M.A. (1985) Growth requirements and the establishment of a chemically defined minimal medium in Zymomonas mobilis. Biotechnol. Lett. 7, 673–678.
22. Sambrook J., and Russell D.W. (2001) Molecular Cloning: Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.
23. Castilho B.A., Olfson P., and Casadaban M.J. (1984) Plasmid insertion mutagenesis and lac gene fusion with mini-Mu bacteriophage transposons. J. Bacteriol. 158, 488–495.