Development of an arabinose-fermenting Zymomonas mobilis strain by metabolic pathway engineering

Applied and Environmental Microbiology

Volumn 62, Issue 12, 1996, Pages 4465-4470

  Deanda, Kristine ^a   Zhang, Min ^a   Eddy, Christina ^a,b   Picataggio, Stephen ^a,c  

^a NATIONAL RENEWABLE ENERGY LABORATORY   (United States)

^b SKIDMORE COLLEGE   (United States)

^c DUPONT COMPANY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARABINOSE; ARABINOSE ISOMERASE; RIBULOKINASE; UNCLASSIFIED DRUG;

AGRICULTURE; ARTICLE; BACTERIAL METABOLISM; BIOMASS; DIFFUSION; ENZYME ISOLATION; FERMENTATION; GENETIC ENGINEERING; GROWTH REGULATION; ZYMOMONAS MOBILIS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; ZYMOMONAS MOBILIS;

EID: 0029910097     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/aem.62.12.4465-4470.1996     Document Type: Article

References (19)

1. Barnell, W. O., K. C. Yi, and T. Conway. 1990. Sequence and genetic organization of a Zymomonas mobilis gene cluster that encodes several enzymes of glucose metabolism. J. Bacteriol. 172:7227-7240.
2. Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-254.
3. Conway, T., G. W. Sewell, and L. O. Ingram. 1987. Glyceraldehyde-3-phosphate dehydrogenase gene from Zymomonas mobilis: cloning, sequencing and identification of the promoter region. J. Bacteriol. 169:5653-5662.
4. DiMarco, A. A., and A. H. Romand. 1985. D-Glucose transport system in Zymomonas mobilis. Appl. Environ. Microbiol. 49:151-157.
5. Feldman, S. D., H. Sahm, and G. A. Sprenger. 1992. Pentose metabolism in Zymomonas mobilis wild-type and recombinant strains. Appl. Microbiol. Biotechnol. 38:354-361.
6. Feldman, S. D., H. Sahm, and G. A. Sprenger. 1922. Cloning and expression of the genes for xylose isomerase and xylulokinase from Klebsiella pneumoniae 1033 in Escherichia coli K12. Mol. Gen. Genet. 234:2111-210.
7. Hinman, N. D., J. D. Wright, W. Hoagland, and C. E. Wyman. 1989. Xylose fermentation: an economic analysis. Appl. Biochem. Biotechnol. 20/21:391-410.
8. Ho, S. N., H. D. Hunt, R. M. Morton, J. K. Pullen, and L. R. Pease. 1989. Site-directed mutagenesis by overlap extension using the polymerase chain reaction. Gene 77:51-59.
9. Ingram, L. O., T. Conway, D. P. Clark, G. W. Sewell, and J. F. Preston. 1987. Genetic engineering of ethanol production in Escherichia coli. Appl. Environ. Microbiol. 53:2420-2425.
10. Lawford, H. G. 1988. A new approach to improving the performance of Zymomonas in continuous ethanol fermentations. Appl. Biochem. Biotechnol. 17:203-219.
11. Lee, N., and E. Englesberg. 1962. Dual effects of structural genes in Escherichia coli. Proc. Natl. Acad. Sci. USA 48:335-348.
12. Lee, N., W. Gielow, R. Martin, E. Hamilton, and A. Fowler. 1986. The organisation of the araBAD operon of Escherichia coli. Gene 47:231-244.
13. McMillan, J. D., and B. L. Boynton. 1994. Arabinose utilization by xylose-fermenting yeasts and fungi. Appl. Biochem. Biotechnol. 45/46:569-584.
14. Millichip, R. J., and H. W. Doelle. 1989. Large-scale ethanol production from milo (sorghum) using Zymomonas mobilis. Proc. Biochem. 24:141-145.
15. Parker, C., W. O. Barnell, J. L. Snoep, L. O. I. Ingram, and T. Conway. 1995. Characterization of the Zymomonas mobilis glucose facilitator gene product (glf) in recombinant Escherichia coli: examination of the transport mechanism, kinetics and role of glucokinase in glucose transport. Mol. Microbiol. 15:795-802.
16. Patrick, J. W., and N. Lee. 1968. Purification and properties of an L-arabinose isomerase from Escherichia coli. J. Biol. Chem. 243:4312-4319.
17. Swings, J., and J. De Ley. 1977. The biology of Zymomonas mobilis. Bacteriol. Rev. 41:1-46.
18. Weisser, P., R. Kramer, H. Sahm, and G. Sprenger. 1995. Functional expression of the glucose transporter of Zymomonas mobilis leads to restoration of glucose and fructose uptake in Escherichia coli mutants and provides evidence for its facilitator action. J. Bacteriol. 177:3351-3354.
19. Zhang, M., C. Eddy, K. Deanda, M. Finkelstein, and S. Picataggio. 1995. Metabolic engineering of a pentose metabolism pathway in ethanologenic Zymomonas mobilis. Science 267:240-243.