Analysis of Androgen Receptor SUMOylation

Methods in Molecular Biology

Volumn 776, Issue , 2011, Pages 183-197

  Rytinki, Miia M ^a   Kaikkonen, Sanna ^a   Sutinen, Päivi ^a   Palvimo, Jorma J ^a  

^a UNIVERSITY OF EASTERN FINLAND   (Finland)

Author keywords

Androgen receptor; cell culture; immunoblotting; immunoprecipitation; lysine residue; post translational modification; prostate cancer cell; steroid hormone; SUMO; transfection

Indexed keywords

ANDROGEN RECEPTOR; TUBULIN;

ANIMAL; ARTICLE; CELL STRAIN COS1; CERCOPITHECUS; CULTURE TECHNIQUE; HUMAN; IMMUNOPRECIPITATION; METABOLISM; POLYACRYLAMIDE GEL ELECTROPHORESIS; SUMOYLATION; TUMOR CELL LINE; WESTERN BLOTTING;

ANIMALS; BLOTTING, WESTERN; CELL CULTURE TECHNIQUES; CELL LINE, TUMOR; CERCOPITHECUS AETHIOPS; COS CELLS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HUMANS; IMMUNOPRECIPITATION; RECEPTORS, ANDROGEN; SUMOYLATION; TUBULIN;

EID: 80052929135     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-61779-243-4_12     Document Type: Chapter

References (11)

1. Gao W, Bohl CE, Dalton JT (2005) Chemistry and structural biology of androgen receptor. Chem Rev 105: 3352–3370
2. Heemers HV, Tindall DJ (2007) Androgen receptor (AR) coregulators: a diversity of functions converging on and regulating the AR transcriptional complex. Endocr Rev 28:778–808
3. Knudsen KE, Scher HI (2009) Starving the addiction: new opportunities for durable suppression of AR signaling in prostate cancer. Clin Cancer Res 15:4792–4798
4. Hay RT (2005) SUMO: a history of modification. Mol Cell 18:1–12
5. Geiss-Friedlander R, Melchior F (2007) Concepts in sumoylation: a decade on. Nat Rev Mol Cell Biol 8:947–956
6. Rytinki M, Kaikkonen S, Pehkonen P, Jääskeläinen T, Palvimo JJ (2009) PIAS proteins: pleiotropic interactors associated with SUMO. Cell Mol Life Sci 66: 3029–3041
7. Hay RT (2007) SUMO-specific proteases: a twist in the tail. Trends Cell Biol 17:370–376
8. Poukka H, Karvonen U, Jänne OA, Palvimo JJ (2000) Covalent modification of the androgen receptor by small ubiquitin-like modifier 1 (SUMO-1). Proc Natl Acad Sci USA 97:14145–14150
9. Kaikkonen S, Jääskeläinen T, Karvonen U, Rytinki M, Makkonen H, Gioeli D, Paschal BM, Palvimo JJ (2009) SUMO-specific protease 1 (SENP1) reverses the hormone-augmented SUMOylation of androgen receptor and modulates gene responses in prostate cancer cells. Mol Endocrinol 23: 292–307
10. Owerbach D, McKay EM, Yeh ET, Gabbay KH, Bohren KM (2005) A proline-90 residue unique to SUMO-4 prevents maturation and sumoylation. Biochem Biophys Res Commun 337:517–520
11. Rytinki MM, Palvimo JJ (2009) SUMOylation attenuates the function of PGC-1α. J Biol Chem 284:26184–26193