Diagnostic efficacy of monoclonal antibody based sandwich enzyme linked immunosorbent assay (ELISA) for detection of Fasciola gigantica excretory/secretory antigens in both serum and stool

Parasites and Vectors

Volumn 4, Issue 1, 2011, Pages

  Demerdash, Zeinab A ^a   Diab, Tarek M ^a   Aly, Ibrahim R ^a   Mohamed, Salwa H ^a   Mahmoud, Faten S ^a   Zoheiry, Mona K ^a   Mansour, Wafaa A ^a   Attia, Mohy E ^b   El Bassiouny, Azza E ^a  

^a Giza   (Egypt)

^b THEODOR BILHARZ RESEARCH INSTITUTE   (Egypt)

Author keywords

Coproantigen; Fasciola gigantica; Monoclonal antibodies; Sandwich ELISA; Seroantigen

Indexed keywords

FASCIOLA GIGANTICA ANTIGEN; IMMUNOGLOBULIN G1; IMMUNOGLOBULIN G2A; MONOCLONAL ANTIBODY; PARASITE ANTIGEN; UNCLASSIFIED DRUG; DIAGNOSTIC AGENT; IMMUNOGLOBULIN G;

ANTIGEN DETECTION; ARTICLE; CONTROLLED CLINICAL TRIAL; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; FASCIOLA GIGANTICA; FASCIOLIASIS; FECES ANALYSIS; FEMALE; HUMAN; MAJOR CLINICAL STUDY; MALE; NON INVASIVE MEASUREMENT; POLYACRYLAMIDE GEL ELECTROPHORESIS; ANIMAL; BAGG ALBINO MOUSE; BLOOD; CHEMISTRY; DIAGNOSIS, MEASUREMENT AND ANALYSIS; EVALUATION; FECES; ISOLATION AND PURIFICATION; METHODOLOGY; MOUSE; SENSITIVITY AND SPECIFICITY; SERUM;

FASCIOLA GIGANTICA;

ANIMALS; ANTIBODIES, MONOCLONAL; ANTIGENS, HELMINTH; CLINICAL LABORATORY TECHNIQUES; ENZYME-LINKED IMMUNOSORBENT ASSAY; FASCIOLIASIS; FECES; HUMANS; IMMUNOGLOBULIN G; MICE; MICE, INBRED BALB C; SENSITIVITY AND SPECIFICITY; SERUM;

EID: 80052829966     PISSN: None     EISSN: 17563305     Source Type: Journal    
DOI: 10.1186/1756-3305-4-176     Document Type: Article

References (40)

1. Epidemiology and control. Torgerson P, Claxton J, Fasciolosis CABI Publishing, Oxon, UK, Dalton JP, 1999 113 149
2. Fascioliasis and other plant-borne trematode zoonoses. Mas-Coma S, Bargues M, Valero M, Int J Parasitol 2005 35 1255 78 10.1016/j.ijpara.2005.07. 010 16150452 (Pubitemid 41395182)
3. Chapter 2. Fasciola, lymnaeids and human fascioliasis, with a global overview on disease transmission, epidemiology, evolutionary genetics, molecular epidemiology and control. Mas-Coma S, Valero MA, Bargues MD, Adv Parasitol 2009 69 41 146 19622408
4. Plant-borne human contamination by fascioliasis. Ashrafi K, Valero MA, Massoud J, Sobhani A, Solaymani-Mohammadi S, Conde P, Khoubbane M, Bargues MD, Mas-Coma S, Am J Trop Med Hyg 2006 75 295 302 16896136 (Pubitemid 46941348)
5. Phenotypic analysis of adults of Fasciola hepatica, Fasciola gigantica and intermediate forms from the endemic region of Gilan. Ashrafi K, Valero MA, Panova M, Periago MV, Massoud J, Mas-Coma S, Iran Parasitol Int 2006 55 249 260 (Pubitemid 44636311)
6. Molecular confirmation that Fasciola gigantica can undertake aberrant migrations in human hosts. Le TH, De NV, Agatsuma T, Blair D, Vercruysse J, Dorny P, Nguyen TG, McManus DP, J Clin Microbiol 2007 45 648 650 10.1128/JCM.01151-06 17135435
7. Genetic characterization, species differentiation and detection of Fasciola spp. by molecular approaches. Lin Ai, Chen Mu-Xin, Alasaad Samer, Elsheikha Hany M, Li Juan, Li Hai-Long, Lin Rui-Qing, Zou Feng-Cai, Zhu Xing-Quan, Chen Jia-Xu, Parasit Vectors 2011 4 101 10.1186/1756-3305-4-101 21658284
8. 27 kDa Fasciola gigantica glycoprotein for the diagnosis of prepatent fasciolosis in cattle. Ghosh S, Rawat P, Velusamy R, Joseph D, Gupta SC, Singh BP, Vet Res Commun 2005 29 123 135 16244937 (Pubitemid 39485503)
9. Comparative diagnostic potentiality of ELISA and dot-ELISA in prepatent diagnosis of experimental Fasciola gigantica infection in cattle. Ghosh S, Rawat P, Gupta SC, Singh BP, Indian J Exp Biol 2005 43 536 541 15991579 (Pubitemid 40816969)
10. Early detection of Fasciola gigantica infection in buffaloes by enzyme-linked immunosorbent assay and dot enzyme-linked immunosorbent assay. Kumar N, Ghosh S, Gupta SC, Parasitol Res 2008 103 141 150 10.1007/s00436-008- 0941-4 18344058
11. Sandwich enzyme-liked immunosorbent assay for detection of excretory/secretory antigens in humans with fascioliasis. Espino A, Finlay C, J Clin Microbiol 1994 32 190 3 8126178 (Pubitemid 24016806)
12. Immunodiagnosis of human and animal fasciolosis. Hillyer GV, Fasciolosis CABI Publishing, Oxon, U.K, Dalton JP, 1999 435 447
13. Detection of coproantigens by sandwich ELISA in sheep experimentally infected with Fasciola gigantica. Estuningsih ES, Widjayanti S, Adiwinata G, Piedrafita D, Trop Biomed 2004 21 Suppl 2 51 56 16493398
14. An ultra-sensitive capture ELISA for detection of Fasciola hepatica coproantigens in sheep and cattle using a new monoclonal antibody (MM3). Mezo M, Gonzalez W, Carro C, Ubeira F, J Parasitol 2004 90 845 52 10.1645/GE-192R 15357080 (Pubitemid 39128744)
15. The use of MM3 monoclonal antibodies for early immunodiagnosis of ovine fascioliasis. Mezo M, González-Warleta M, Ubeira FM, J Parasitol 2007 93 65 72 10.1645/GE-925R.1 17436943 (Pubitemid 46625540)
16. The MIF stain preservative technique for the identification of intestinal parasites. Sapero JJ, Lawless DK, Am J Trop Med Hyg 1953 2 613 619 13065626
17. Morbidity in schistosomiasis mansoni in relation to intensity of infection: A study of a community in Machakos, Kenya. Siongok TKA, Mahmoud AAF, Guma JH, Warren KS, Muller AS, Handa AK, Houser HB, Am J Trop Med Hyg 1976 25 273 284 1259088
18. Dynamics of antigenemia and coproantigens during a human Fasciola hepatica outbreak. Espino A, Diaz A, Perez A, Carlos M, J Clin Microbiol 1998 36 2723 6 9705421 (Pubitemid 28380984)
19. Thiol protease released in vitro by Fasciola hepatica. Dalton JP, Heffernan M, Mol Biochem Parasitol 1989 35 161 6 10.1016/0166-6851(89)90118-7 2770786 (Pubitemid 19157025)
20. Simple immunization protocol for high frequency production of soluble antigen specific hybridomas. Cianfriglia M, Armellini D, Massone A, Mariani M, Hybridoma 1983 2 451 7 10.1089/hyb.1983.2.451 6678805 (Pubitemid 14140186)
21. Properties of monoclonal antibodies: Strategies and procedures. Galfre G, Milstein C, Meth Enzymol 1981 73 3 46 7300683
22. Detection of circulating schistosome antigens in serum and urine of schistosomiasis patients and assessment of cure by a monoclonal antibody. Demerdash Z, Mahmoud S, Shaker Z, Hassan S, Mansour M, J Egypt Soc Parasitol 1995 25 471 84 7665943
23. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Laemmli U, Nature 1970 227 680 5 10.1038/227680a0 5432063
24. Enzyme-Linked Immunoelectro-Transfer Blot Techniques (EITB) for Studying the Specificities of Antigens and Antibodies Separated by Gel Electrophoresis. Tsang V, Peralta J, Simons A, Methods in Enzymology: Immunochemical Techniques Academic Press, New York, Langone JJ, Vunakie HV, 1983 337 91
25. Biochemical characterization and localization of Fasciola hepatica 26-28 kDa diagnostic coproantigen. Abdel Rahman S, Malone J, O'reilly K, Parasite Immunol 1999 21 279 86 10.1046/j.1365-3024.1999.00191.x 10354350 (Pubitemid 29257756)
26. Detection of monoclonal antibodies specific for carbohydrate epitopes using periodate oxidation. Woodward M, Young W, Bloodgood R, J Immunol Meth 1985 78 143 53 10.1016/0022-1759(85)90337-0 (Pubitemid 15076753)
27. Purification of Antigens and Antibodies. Maizels RM, Robertson BD, Imperial College of Science, Technology and Medicine, London 3 1988
28. Highly efficient and simple methods for the preparation of peroxidase and active peroxidase-antibody conjugates for enzyme immunoassays. Tijssen P, Kurstak P, Anal Biochem 1984 136 451 7 10.1016/0003-2697(84)90243-4 6372541
29. Predictive values and efficiency of laboratory testing. Galen RS, Pediat J Clin North Am 1980 27 861 69 (Pubitemid 11183845)
30. Detection of Fasciola-specific excretory/secretory (E/S) protein fraction band (49.5 kDa) and its utilization in diagnosis of early fascioliasis using different diagnostic techniques. Arafa MS, Abaza SM, El-Shewy KA, Mohareb EW, El-Moamly AA, J Egypt Soc Parasitol 1999 29 Suppl 3 911 26 12561930
31. Fasciola gigantica: Immunodiagnosis of fasciolosis by detection of circulating 28.5 kDa tegumental antigen. Anuracpreeda P, Wanichanon C, Chawengkirtikul R, Chaithirayanon K, Sobhon P, Exp Parasitol 2009 123 334 340 10.1016/j.exppara.2009.08.014 19723524
32. MM3-ELISA detection of Fasciola hepatica coproantigens in preserved human stool samples. Ubeira FM, Muĩo L, Valero MA, Periago MV, Pérez-Crespo I, Mezo M, González-Warleta M, Romarís F, Paniagua E, Cortizo S, Llovo J, Más-Coma S, Am J Trop Med Hyg 2009 81 Suppl 1 156 62 19556582
33. Comparison of an indirect haemagglutination assay and an ELISA for diagnosing Fasciola hepatica in experimentally and naturally infected sheep. Cornelissen JB, de Leeuw WA, van der Heijden PJ, Vet Quart 1992 14 152 156
34. Kinetics of antibody-based antigen detection in serum and faeces of sheep experimentally infected with Fasciola hepatica. Dumenigo BE, Espino AM, Finlay CM, Mezo M, Vet Parasitol 2000 89 153 161 10.1016/S0304-4017(00)00206-5 10729655 (Pubitemid 30151116)
35. Fasciola hepatica: Comparison of flukes from Korea and the United States by isoelectric focusing banding patterns of whole-body protein. Lee CG, Zimmerman GL, Wee SH, Vet Parasitol 1992 42 Suppl 3-4 311 6 1496791
36. Cysteine proteinases Fas1 and Fas2 are diagnostic markers for Fasciola hepatica infection in alpacas (Lama pacos). Neyra V, Chavarry E, Espinoza JR, Vet Parasitol 2002 105 21 32 10.1016/S0304-4017(02)00002-X 11879964 (Pubitemid 34195571)
37. Detection of circulating 54 kDa antigen in sera of bovine calves experimentally infected with F. gigantica. Velusamy R, Singh BP, Sharma RL, Chanda D, Vet Parasitol 2004 119 187 195 10.1016/j.vetpar.2003.09.023 14746978 (Pubitemid 38121390)
38. MM3-ELISA evaluation of coproantigen release and serum antibody production in sheep experimentally infected with Fasciola hepatica and F. gigantica. Valero MA, Ubeira FM, Khoubbane M, Artigas P, Muino L, Mezo M, Pérez-Crespo I, Periago MV, Mas-Coma S, Vet Parasitol 2009 159 77 81 10.1016/j.vetpar.2008.10.014 19019548
39. Fasciola gigantica: immunolocalization of 28.5 kDa antigen in the tegument of metacercaria and juvenile fluke. Anuracpreeda P, Wanichanon C, Sobhon P, Exp Parasitol 2009 122 75 83 10.1016/j.exppara.2009.03.007 19303872
40. Detection of Fasciola hepatica antigens in cattle feces by a monoclonal antibody-based sandwich immunoassay. Dumenigo B, Espino A, Finlay C, Res Vet Sci 1996 60 278 9 10.1016/S0034-5288(96)90055-7 8735523 (Pubitemid 126501609)