Highly sensitive direct detection and quantification of Burkholderia pseudomallei bacteria in environmental soil samples by using real-time PCR

Applied and Environmental Microbiology

Volumn 77, Issue 18, 2011, Pages 6486-6494

  Trung, Trinh Thanh ^a   Hetzer, Adrian ^a   Göhler, André ^a   Topfstedt, Eylin ^a   Wuthiekanun, Vanaporn ^b   Limmathurotsakul, Direk ^b   Peacock, Sharon J ^b,c   Steinmetz, Ivo ^a  

^a UNIVERSITY OF GREIFSWALD   (Germany)

^b MAHIDOL UNIVERSITY   (Thailand)

^c UNIVERSITY OF CAMBRIDGE   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL COUNT; BIOTHREAT AGENTS; BURKHOLDERIA PSEUDOMALLEI; DIRECT DETECTION; ENVIRONMENTAL DETECTION; ENVIRONMENTAL SURVEILLANCE; GENOME EQUIVALENT; GEOMETRIC MEAN; HIGHLY SENSITIVE; INFECTIOUS DISEASE; MELIOIDOSIS; NESTED PCR; NORTHEAST THAILAND; PCR METHOD; QUANTITATIVE PCR; REAL-TIME PCR; SINGLE-COPY GENES; SOIL BACTERIUM; SOIL DNA; SOIL SAMPLE; TYPE THREE SECRETION SYSTEMS;

AGRICULTURE; BACTERIA; CHEMICAL SENSORS; DNA; GENES; SOIL SURVEYS; SOILS;

POLYMERASE CHAIN REACTION;

BACTERIAL DNA; CARRIER PROTEIN;

BACTERIOLOGY; BACTERIUM; BIOASSAY; DETECTION METHOD; DISEASE PREVALENCE; GENOME; INFECTIVITY; LIVESTOCK; POLYMERASE CHAIN REACTION; QUANTITATIVE ANALYSIS; SENSITIVITY ANALYSIS; SOIL MICROORGANISM; SOIL TYPE;

ARTICLE; BURKHOLDERIA PSEUDOMALLEI; COMPARATIVE STUDY; GENETICS; HUMAN; ISOLATION AND PURIFICATION; METHODOLOGY; MICROBIOLOGICAL EXAMINATION; MICROBIOLOGY; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; THAILAND; VALIDATION STUDY;

BACTERIOLOGICAL TECHNIQUES; BURKHOLDERIA PSEUDOMALLEI; DNA, BACTERIAL; HUMANS; MEMBRANE TRANSPORT PROTEINS; REAL-TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SOIL MICROBIOLOGY; THAILAND;

THAILAND;

BACTERIA (MICROORGANISMS); BURKHOLDERIA PSEUDOMALLEI;

EID: 80052817881     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.00735-11     Document Type: Article

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