Using the Flp-In™ T-Rex™ system to regulate GPCR expression

Methods in Molecular Biology

Volumn 746, Issue , 2011, Pages 21-37

  Ward, Richard J ^a   Alvarez Curto, Elisa ^a   Milligan, Graeme ^a  

^a UNIVERSITY OF GLASGOW   (United Kingdom)

Author keywords

Doxycycline induction; Flp In T Rex ; GPCR expression; Inducible locus; Stable expression; Tet repressor

Indexed keywords

G PROTEIN COUPLED RECEPTOR; DNA POLYMERASE; RECOMBINASE; REPRESSOR PROTEIN; TETRACYCLINE; TETRACYCLINE RESISTANCE ENCODING TRANSPOSON REPRESSOR PROTEIN; TETRACYCLINE RESISTANCE-ENCODING TRANSPOSON REPRESSOR PROTEIN;

ARTICLE; CELL CULTURE; CELL POPULATION; CONTROLLED STUDY; CULTURE MEDIUM; GENETIC TRANSFECTION; GENOME; HUMAN; HUMAN CELL; OLIGOMERIZATION; PRIORITY JOURNAL; PROTEIN EXPRESSION; SIGNAL TRANSDUCTION; CELL CLONE; CELL STRAIN HEK293; DRUG EFFECT; GENE EXPRESSION REGULATION; GENE VECTOR; GENETIC PROCEDURES; GENETICS; METABOLISM; TRANSCRIPTION INITIATION;

MAMMALIA;

CLONE CELLS; DNA NUCLEOTIDYLTRANSFERASES; GENE EXPRESSION REGULATION; GENETIC TECHNIQUES; GENETIC VECTORS; HEK293 CELLS; HUMANS; RECEPTORS, G-PROTEIN-COUPLED; REPRESSOR PROTEINS; TETRACYCLINE; TRANSCRIPTIONAL ACTIVATION; TRANSFECTION;

EID: 80052811601     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-126-0_2     Document Type: Article

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