An efficient and rapid method for cDNA cloning from difficult templates using codon optimization and SOE-PCR: With human RANK and TIMP2 gene as examples

Biotechnology Letters

Volumn 33, Issue 10, 2011, Pages 1939-1947

  Huang, Gang ^a   Wen, Qianjun ^a   Gao, Qiangguo ^a   Zhang, Fang ^b   Bai, Yun ^a  

^a THIRD MILITARY MEDICAL UNIVERSITY   (China)

^b MEDICAL SCHOOL OF NANJING UNIVERSITY   (China)

Author keywords

Codon optimization; GC content; Metalloproteinase inhibitor 2; Receptor activator of NF B; Splicing by overlapping extension

Indexed keywords

CDNA CLONING; CDS; CODING SEQUENCES; CODON OPTIMIZATION; EUKARYOTIC EXPRESSION; GC CONTENT; GC CONTENTS; GENE CLONING; METALLOPROTEINASES; RAPID METHOD; RECEPTOR ACTIVATOR; TARGET GENES; WESTERN BLOTTING;

CLONING; OPTIMIZATION;

GENES;

COMPLEMENTARY DNA; RECEPTOR ACTIVATOR OF NUCLEAR FACTOR KAPPA B; TIMP2 PROTEIN, HUMAN; TISSUE INHIBITOR OF METALLOPROTEINASE 2; TNFRSF11A PROTEIN, HUMAN;

AMINO ACID SEQUENCE; ARTICLE; CARCINOMA; CODON; COLON TUMOR; DNA BASE COMPOSITION; DNA SEQUENCE; EXON; GENETICS; HUMAN; METABOLISM; METHODOLOGY; MOLECULAR CLONING; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PLASMID; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; TUMOR CELL LINE;

AMINO ACID SEQUENCE; BASE COMPOSITION; BASE SEQUENCE; CARCINOMA; CELL LINE, TUMOR; CLONING, MOLECULAR; CODON; COLONIC NEOPLASMS; DNA, COMPLEMENTARY; EXONS; HUMANS; MOLECULAR SEQUENCE DATA; PLASMIDS; RECEPTOR ACTIVATOR OF NUCLEAR FACTOR-KAPPA B; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS, DNA; TISSUE INHIBITOR OF METALLOPROTEINASE-2;

EUKARYOTA;

EID: 80052794423     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-011-0656-y     Document Type: Article

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