Comparison of molecular and McMaster microscopy techniques to confirm the presence of naturally acquired strongylid nematode infections in sheep

Molecular and Biochemical Parasitology

Volumn 180, Issue 1, 2011, Pages 62-67

  Sweeny, Joshua P A ^a   Robertson, Ian D ^a   Ryan, Una M ^a   Jacobson, Caroline ^a   Woodgate, Rob G ^b  

^a MURDOCH UNIVERSITY   (Australia)

^b DEPARTMENT OF AGRICULTURE AND FOOD WESTERN AUSTRALIA   (Australia)

Author keywords

Diagnosis; DNA extraction; McMaster faecal worm egg count; PCR; Sheep; Strongylid nematodes

Indexed keywords

GENOMIC DNA; RIBOSOME DNA;

ARTICLE; CHABERTIA OVINA; CONTROLLED STUDY; DIAGNOSTIC TEST; DNA EXTRACTION; DNA SCREENING; FECES ANALYSIS; HAEMONCHUS CONTORTUS; INTERMETHOD COMPARISON; LAMB; MICROSCOPY; NONHUMAN; PARASITE IDENTIFICATION; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SHEEP DISEASE; STRONGYLE INFECTION; STRONGYLOIDEA; TELADORSAGIA CIRCUMCINCTA; TRICHOSTRONGYLUS COLUBRIFORMIS; WORM EGG;

ANIMALS; FECES; MICROSCOPY; POLYMERASE CHAIN REACTION; SHEEP; SHEEP DISEASES; STRONGYLIDA; STRONGYLIDA INFECTIONS;

CHABERTIA OVINA; HAEMONCHUS CONTORTUS; NEMATODA; OESOPHAGOSTOMUM; OVIS ARIES; STRONGYLIDA; TELADORSAGIA CIRCUMCINCTA; TRICHOSTRONGYLUS COLUBRIFORMIS;

EID: 80052779629     PISSN: 01666851     EISSN: 18729428     Source Type: Journal    
DOI: 10.1016/j.molbiopara.2011.07.007     Document Type: Article

References (18)

1. R.B. Besier, and S.C.J. Love Anthelmintic resistance in sheep nematodes in Australia: the need for new approaches Aust J Exp Agric 43 2003 1383 1391 (Pubitemid 38171150)
2. R.G. Woodgate, and R.B. Besier Sustainable use of anthelmintics in an integrated parasite management program for sheep nematodes Anim Prod Sci 50 2010 440 443
3. H.V. Whitlock Some modifications of the McMaster helminth egg counting technique and apparatus J Council Sci Ind Res Aust 21 1948 177 180
4. P. Kingsbury Relationship between egg counts and worm burdens of young sheep Vet Rec 77 1965 900 901
5. R.J. Dobson, E.H. Barnes, S.D. Birclijin, and J.H. Gill The survival of Ostertagia circumcincta and Trichostrongylus colubriformis in faecal culture as a source of bias in apportioning egg counts to worm species Int J Parasitol 22 1992 1005 1008
6. N.J. Bott, B.E. Campbell, and I. Beveridge A combined microscopic-molecular method for the diagnosis of strongylid infections in sheep Int J Parasitol 39 2009 1277 1287
7. F. Roeber, A.R. Jex, A.J.D. Campbell, B.E. Campbell, G.A. Anderson, and R.B. Gasser Evaluation and application of a molecular method to assess the composition of strongylid nematode populations in sheep with naturally acquired infections Infect Genet Evol 11 2011 849 854
8. Sweeny JPA, Ryan UM, Robertson ID, Jacobson C. Validation and application of a molecular diagnostic technique for identification of naturally acquired strongylid nematode infections in lambs using faecal DNA extractions. Vet J, in press.
9. C. Moeller, I. Smith, S. Asseng, F. Ludwig, and N. Telcik The potential value of seasonal forecasts of rainfall categories - case studies from the wheatbelt in Western Australia's Mediterranean region Agric For Meteorol 148 2008 606 618
10. M. Lyndal-Murphy Anthelmintic resistance in sheep L.A. Corner, T.J. Bagust, Australian standard diagnostic techniques for animal diseases 1993 CSIRO for the Standing Committee on Agriculture and Resource Management Melbourne
11. J.P.A. Sweeny, U.M. Ryan, I.D. Robertson, and C. Jacobson Cryptosporidium and Giardia associated with reduced lamb carcase productivity Vet Parasitol 2011 10.1016/j.vetpar.2011.05.050
12. J.P.A. Sweeny, U.M. Ryan, I.D. Robertson, R. Yang, K. Bell, and C. Jacobson Longitudinal investigation of protozoan parasites in meat lamb farms in southern Western Australia Prev Vet Med 101 2011 192 203
13. N. Anderson Trichostrongylid infections of sheep in a winter rainfall region - I. Epizootiological studies in the western district of Victoria, 1966-1967 Aust J Agric Res 23 1972 1113 1129
14. B. Sinniah Daily egg production of Ascaris lumbricoides: the distribution of eggs in the faeces and the variability of egg counts Parasitology 84 1982 167 175 (Pubitemid 12154908)
15. J.D. Dargie, and E.W. Allonby Pathophysiology of single and challenge infections of Haemonchus contortus in Merino sheep: studies on red cell kinetics and the self-cure phenomenon Int J Parasitol 5 1975 147 157
16. D.G. Palmer, and I.L. McCombe Lectin staining of trichostrongylid nematode eggs of sheep: rapid identification of Haemonchus contortus eggs with peanut agglutinin Int J Parasitol 26 1996 447 450 (Pubitemid 26167525)
17. M.E. Jurasek, J.K. Bishop-Stewart, B.E. Storey, R.M. Kaplan, and M.L. Kent Modification and further evaluation of a fluorescein-labeled peanut agglutinin test for identification of Haemonchus contortus eggs Vet Parasitol 169 2010 209 213
18. I.G. Colditz, and L.F. Le Jambre Development of a faecal occult blood test to determine the severity of Haemonchus contortus infections in sheep Vet Parasitol 153 2008 93 99