Determination of the catalytic base in family 48 glycosyl hydrolases

Applied and Environmental Microbiology

Volumn 77, Issue 17, 2011, Pages 6274-6276

  Kostylev, Maxim ^a   Wilson, David B ^a  

^a Department of Obstetrics Gynecology   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ASPARTIC ACIDS; CATALYTIC BASE; GLYCOSYL HYDROLASES; MODELING DATA; RESCUE ACTIVITIES; SITE DIRECTED MUTAGENESIS; THERMOBIFIDA FUSCA;

AMINO ACIDS;

HYDROLASES;

GLYCOSIDASE; MUTANT PROTEIN;

CATALYST; ENZYME ACTIVITY; EXPERIMENTAL STUDY; MUTAGENICITY; SUBSTRATE;

ACTINOMYCETALES; AMINO ACID SUBSTITUTION; ARTICLE; ENZYME ACTIVE SITE; ENZYMOLOGY; GENETICS; METABOLISM; SITE DIRECTED MUTAGENESIS;

ACTINOMYCETALES; AMINO ACID SUBSTITUTION; CATALYTIC DOMAIN; GLYCOSIDE HYDROLASES; MUTAGENESIS, SITE-DIRECTED; MUTANT PROTEINS;

THERMOBIFIDA FUSCA;

EID: 80052705055     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.05532-11     Document Type: Article

References (19)

1. Berger, E., D. Zhang, V. V. Zverlov, and W. H. Schwarz. 2007. Two noncellulosomal cellulases of Clostridium thermocellum, Cel9I and Cel48Y, hydrolyse crystalline cellulose synergistically. FEMS Microbiol. Lett. 268:194-201.
2. Guimarãs, B. G., H. Souchon, B. L. Lytle, J. H. David Wu, and P. M. Alzari. 2002. The crystal structure and catalytic mechanism of cellobiohydrolase CelS, the major enzymatic component of the Clostridium thermocellum cellulosome. J. Mol. Biol. 320:587-596.
3. Irwin, D. C., S. Zhang, and D. B. Wilson. 2000. Cloning, expression and characterization of a family 48 exocellulase, Cel48A, from Thermobifida fusca. Eur. J. Biochem. 267:4988-4997.
4. Kruus, K., W. K. Wang, J. Ching, and J. H. Wu. 1995. Exoglucanase activities of the recombinant Clostridium thermocellum CelS, a major cellulosome component. J. Bacteriol. 177:1641-1644.
5. MacLeod, A. M., T. Lindhorst, S. G. Withers, and R. A. Warren. 1994. The acid/base catalyst in the exoglucanase/xylanase from Cellulomonas fimi is glutamic acid 127: evidence from detailed kinetic studies of mutants. Biochemistry 33:6371-6376.
6. Merino, S. T., and J. Cherry. 2007. Progress and challenges in enzyme development for biomass utilization. Adv. Biochem. Eng. Biotechnol. 108:95-120.
7. Olson, D. G., et al. 2010. Deletion of the Cel48S cellulase from Clostridium thermocellum. Proc. Natl. Acad. Sci. U. S. A. 107:17727-17732.
8. Parsiegla, G., et al. 1998. The crystal structure of the processive endocellulase CelF of Clostridium cellulolyticum in complex with a thiooligosaccharide inhibitor at 2.0 A resolution. EMBO J. 17:5551-5562.
9. Parsiegla, G., et al. 2000. Crystal structures of the cellulase Cel48F in complex with inhibitors and substrates give insights into its processive action. Biochemistry 39:11238-11246.
10. 10. Parsiegla, G., C. Reverbel, C. Tardif, H. Driguez, and R. Haser. 2008. Structures of mutants of cellulase Cel48F of Clostridium cellulolyticum in complex with long hemithiocellooligosaccharides give rise to a new view of the substrate pathway during processive action. J. Mol. Biol. 375:499-510.
11. Perlack, R. D., et al. 2005. Biomass as feedstock for a bioenergy and bioproducts industry: the technical feasibility of a billion-ton annual supply. Oak Ridge National Laboratory, Oak Ridge, TN.
12. Reverbel-Leroy, C., S. Pages, A. Belaich, J. P. Belaich, and C. Tardif. 1997. The processive endocellulase CelF, a major component of the Clostridium cellulolyticum cellulosome: purification and characterization of the recombinant form. J. Bacteriol. 179:46-52.
13. Saharay, M., H. Guo, and J. C. Smith. 2010. Catalytic mechanism of cellulose degradation by a cellobiohydrolase, CelS. PLoS One 5:e12947.
14. Shen, H., et al. 1994. Stereochemical course of hydrolysis catalysed by Cellulomonas fimi CenE, a member of a new family of beta-1,4-glucanases. Biochem. Biophys. Res. Commun. 199:1223-1228.
15. Spiridonov, N. A., and D. B. Wilson. 1998. Regulation of biosynthesis of individual cellulases in Thermomonospora fusca. J. Bacteriol. 180:3529- 3532.
16. Wen, F., N. U. Nair, and H. Zhao. 2009. Protein engineering in designing tailored enzymes and microorganisms for biofuels production. Curr. Opin. Biotechnol. 20:412-419.
17. Wu, J. H. D., W. H. Orme-Johnson, and A. L. Demain. 1988. Two components of an extracellular protein aggregate of Clostridium thermocellum together degrade crystalline cellulose. Biochemistry 27:1703-1709.
18. Zhang, Y. H., J. Cui, L. R. Lynd, and L. R. Kuang. 2006. A transition from cellulose swelling to cellulose dissolution by o-phosphoric acid: evidence from enzymatic hydrolysis and supramolecular structure. Biomacromolecules 7:644-648.
19. Zverlov, V. V., J. Kellermann, and W. H. Schwarz. 2005. Functional subgenomics of Clostridium thermocellum cellulosomal genes: identification of the major catalytic components in the extracellular complex and detection of three new enzymes. Proteomics 5:3646-3653.