Entrapment of Enzymes in Nanoporous Sol–Gels

Methods in Molecular Biology

Volumn 743, Issue , 2011, Pages 223-237

  Buthe, Andreas ^a  

^a UNIVERSITY OF MINNESOTA   (United States)

Author keywords

alkoxysilanes; biodiesel; entrapment; Enzyme immobilization; lipase; nanoporous silica glass; silanes; sol gel process; transesterification

Indexed keywords

CANOLA OIL; IMMOBILIZED ENZYME; METHANOL; MONOUNSATURATED FATTY ACID; NANOMATERIAL; SILANE DERIVATIVE; SILICA GEL; SILICON DIOXIDE; TRIACYLGLYCEROL LIPASE; TRIMETHOXYSILANE;

ARTICLE; CHEMISTRY; ESTERIFICATION; FUNGUS; METABOLISM; PHASE TRANSITION; POROSITY;

ENZYMES, IMMOBILIZED; ESTERIFICATION; FATTY ACIDS, MONOUNSATURATED; FUNGI; LIPASE; METHANOL; NANOSTRUCTURES; PHASE TRANSITION; POROSITY; SILANES; SILICA GEL; SILICON DIOXIDE;

EID: 80052623565     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-61779-132-1_18     Document Type: Chapter

References (32)

1. Ansorge-Schumacher, M. B. (2008) Immobilization of biological catalysts. Handbook of Heterogeneous Catalysis, 2nd Edn., Vol. 1, pp. 644–655.
2. Hanefeld, U., Gardossi, L., and Magner, E. (2009) Understanding enzyme immobilization. Chem. Soc. Rev. 38, 453–468.
3. Reetz, M. T., Tielmann, P., Wiesenhoefer, W., Koenen, W., and Zonta, A. (2003) Second generation sol-gel encapsulated lipases: Robust heterogeneous biocatalysts. Adv. Synth. Catal. 345, 717–728.
4. Noureddini, H., and Gao, X. (2007) Characterization of sol-gel immobilized lipases. J. Sol-Gel Sci. Technol. 41, 31–41.
5. Dunker, A. K., and Fernandez, A. (2007) Engineering productive enzyme confinement. Trends Biotechnol. 25, 189–190.
6. Keeling-Tucker, T., Rakic, M., Spong, C., and Brennan, J. D. (2000) Controlling the material properties and biological activity of lipase within sol-gel derived bioglasses via organosilane and polymer doping. Chem. Mater. 12, 3695–3704.
7. Gupta, R., and Chaudhury, N. K. (2007) Entrapment of biomolecules in sol-gel matrix for applications in biosensors: Problems and future prospects. Biosens. Bioelectron. 22, 2387–2399.
8. Kandimalla, V. B., Tripathi, V. S., and Ju, H. (2006) Immobilization of biomolecules in sol-gels: Biological and analytical applications. Crit. Rev. Anal. Chem. 36, 73–106.
9. Lan, E. H., Dunn, B., and Zink, J. I. (2005) Nanostructured systems for biological materials. Methods Mol. Biol. 300, 53–79.
10. El-Zahab, B., Jia, H., and Wang, P. (2004) Enabling multienzyme biocatalysis using nanoporous materials. Biotechnol. Bioeng. 87, 178–183.
11. Dave, B. C., Dunn, B., Valentine, J. S., and Zink, J. I. (1994) Sol-gel encapsulation methods for biosensors. Anal. Chem. 66, 1120A–1127A.
12. Noureddini, H., Gao, X., Joshi, S., and Wagner, P. R. (2002) Immobilization of Pseu-domonas cepacia lipase by sol-gel entrapment and its application in the hydrolysis of soybean oil. J. Am. Oil Chem. Soc. 79, 33–40.
13. Wang, P. (2006) Nanoscale biocatalyst systems. Curr. Opin. Biotechnol. 17, 574–579.
14. Wang, P., Dai, S., Waezsada, S. D., Tsao, A. Y., and Davison, B. H. (2001) Enzyme stabilization by covalent binding in nanoporous sol-gel glass for nonaqueous biocatalysis. Biotechnol. Bioeng. 74, 249–255.
15. Schmidt, H., Scholze, H., and Kaiser, A. (1984) Principles of hydrolysis and condensation reaction of alkoxysilanes. J. Non-Cryst. Solids 63, 1–11.
16. Wilkes, G. L., Orler, B., and Huang, H. H. (1985) “Ceramers”: Hybrid materials incorporating polymeric/oligomeric species into inorganic glasses utilizing a sol-gel approach. Polym. Prepr. (Am. Chem. Soc., Div. Polym. Chem.) 26, 300–302.
17. Park, T., Iwuoha, E. I., Smyth, M. R., and MacCraith, B. D. (1996) Sol-gel-based amperometric glucose biosensor incorporating an osmium redox polymer as mediator. Anal. Commun. 33, 271–273.
18. Siu, E., Won, K., and Park, C. B. (2007) Electrochemical regeneration of NADH using conductive vanadia-silica xerogels. Biotechnol. Prog. 23, 293–296.
19. Li, W., Yuan, R., Chai, Y., Zhou, L., Chen, S., and Li, N. (2008) Immobilization of horseradish peroxidase on chitosan/silica sol-gel hybrid membranes for the preparation of hydrogen peroxide biosensor. J. Biochem. Biophys. Methods 70, 830–837.
20. Saxena, R. K., Ghosh, P. K., Gupta, R., Davidson, W. S., Bradoo, S., and Gulati, R. (1999) Microbial lipases: Potential biocatalysts for the future industry. Curr. Sci. 77, 101–115.
21. Bradford, M. M. (1976) A rapid and sensitive method for the quantitation of micro-gram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248–254.
22. Kuncova, G., Szilva, J., Hetflejs, J., and Sabata, S. (2003) Catalysis in organic solvents with lipase immobilized by sol-gel technique. J. Sol-Gel Sci. Technol. 26, 1183–1187.
23. Reetz, M. T., Zonta, A., and Simpelkamp, J. (1996) Efficient immobilization of lipases by entrapment in hydrophobic sol-gel materials. Biotechnol. Bioeng. 49, 527–534.
24. Chen, J., and Lin, W. (2003) Sol-gel powders and supported sol-gel polymers for immobilization of lipase in ester synthesis. Enzyme. Microb. Technol. 32, 801–811.
25. Lei, C., Soares, T. A., Shin, Y., Liu, J., and Ackerman, E. J. (2008) Enzyme specific activity in functionalized nanoporous supports. Nanotechnology 19, 125102/ 1–125102/9.
26. Sing, K. S. W. (1998) Adsorption methods for the characterization of porous materials. Adv. Colloid Interface Sci. 76–77, 3–11.
27. Wei, Y., and Qiu, K. (2000) Synthesis and biotechnological applications of vinyl polymer-inorganic hybrid and mesoporous materials. Chin. J. Polym. Sci. 18, 1–7.
28. Zaks, A., and Klibanov, A. M. (1984) Enzymatic catalysis in organic media at 100 degrees C. Science 224, 1249–1251.
29. Parawira, W. (2009) Biotechnological production of biodiesel fuel using biocatal-ysed transesterification: A review. Crit. Rev. Biotechnol. 29, 82–93.
30. Knothe, G. (2001) Analytical methods used in the production and fuel quality assessment of biodiesel. Trans. ASAE 44, 193–200.
31. Mittelbach, M., Roth, G., and Bergmann, A. (1996) Simultaneous gas chromatographic determination of methanol and free glycerol in biodiesel. Chromatographia 42, 431–434.
32. Martyanov, I. N., and Sayari, A. (2008) Comparative study of triglyceride transesterification in the presence of catalytic amounts of sodium, magnesium, and calcium methox-ides. Appl. Catal. A 339, 45–52.