Purification and characterization of the [NiFe]-Hydrogenase of Shewanella oneidensis MR-1

Applied and Environmental Microbiology

Volumn 77, Issue 16, 2011, Pages 5584-5590

  Shi, Liang ^a   Belchik, Sara M ^a   Plymale, Andrew E ^a   Heald, Steve ^b   Dohnalkova, Alice C ^a   Sybirna, Kateryna ^c   Bottin, Hervé ^c   Squier, Thomas C ^a   Zachara, John M ^a   Fredrickson, James K ^a  

^a PACIFIC NORTHWEST NATIONAL LABORATORY   (United States)

^b ARGONNE NATIONAL LABORATORY   (United States)

^c SERVICE DE CHIMIE BIOORGANIQUE ET DE MARQUAGE   (France)

Author keywords

[No Author keywords available]

Indexed keywords

[NIFE]-HYDROGENASE; BONDING ENVIRONMENT; EXTENDED X-RAY ADSORPTION FINE STRUCTURE; GENES ENCODING; HEPES BUFFER; METHYL VIOLOGEN; REDUCTION PRODUCTS; SHEWANELLA ONEIDENSIS MR-1; TRANSMISSION ELECTRON; TRIS BUFFERS; WILD TYPES; X-RAY ABSORPTION NEAR EDGE SPECTROSCOPY;

ABSORPTION SPECTROSCOPY; ADSORPTION; BACTERIA; GENES; OXIDATION; TECHNETIUM; TRANSMISSION ELECTRON MICROSCOPY;

PURIFICATION;

BUFFER; HYDROGEN; HYDROGENASE; NICKEL IRON HYDROGENASE; NICKEL-IRON HYDROGENASE; PARAQUAT; TECHNETIUM; TECHNETIUM COMPLEX;

BACTERIUM; CELL ORGANELLE; DIAMETER; ENZYME ACTIVITY; GENE EXPRESSION; MEASUREMENT METHOD; MUTATION; OXIDATION; RECOMBINATION; SOLUBILIZATION; TRANSMISSION ELECTRON MICROSCOPY; X-RAY SPECTROSCOPY;

ARTICLE; BACTERIAL GENE; ENZYMOLOGY; GENETIC COMPLEMENTATION; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; MOLECULAR CLONING; OXIDATION REDUCTION REACTION; SHEWANELLA; SPECTROMETRY; TRANSMISSION ELECTRON MICROSCOPY; WESTERN BLOTTING;

BLOTTING, WESTERN; BUFFERS; CLONING, MOLECULAR; GENES, BACTERIAL; GENETIC COMPLEMENTATION TEST; HYDROGEN; HYDROGENASE; MICROSCOPY, ELECTRON, TRANSMISSION; ORGANOTECHNETIUM COMPOUNDS; OXIDATION-REDUCTION; PARAQUAT; SHEWANELLA; SPECTROMETRY, X-RAY EMISSION; TECHNETIUM;

SHEWANELLA ONEIDENSIS MR-1;

EID: 80052618794     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.00260-11     Document Type: Article

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