Expression of metallocarboxypeptidase inhibitors in Escherichia coli: Effect of cysteine content and protein size in the secretory production of disulfide-bridged proteins

Journal of Industrial Microbiology and Biotechnology

Volumn 38, Issue 9, 2011, Pages 1553-1560

  Puertas, Juan Miguel ^a   Caminal, Glòria ^a   González, Glòria ^a  

^a UNIVERSITAT AUTÒNOMA DE BARCELONA   (Spain)

Author keywords

Disulfide bridges; High cell density cultivation; Recombinant secretion

Indexed keywords

ARABINOSE; CARBON; CARBOXYPEPTIDASE; CYSTEINE; DSBA PROTEIN; GLYCEROL; LEECH CARBOXYPEPTIDASE INHIBITOR; POTATO CARBOXYPEPTIDASE INHIBITOR; TICK CARBOXYPEPTIDASE INHIBITOR; UNCLASSIFIED DRUG;

ARTICLE; CARBON SOURCE; CELL CULTURE; CELL DENSITY; CELL MEMBRANE PERMEABILITY; CELL STRAIN; CONTROLLED STUDY; CYTOPLASM; ESCHERICHIA COLI; GROWTH RATE; NONHUMAN; PROTEIN ANALYSIS; PROTEIN CONTENT; PROTEIN EXPRESSION; PROTEIN SECRETION;

CARBOXYPEPTIDASES; CYSTEINE; DISULFIDES; ESCHERICHIA COLI; PERIPLASM; PROTEASE INHIBITORS; PROTEIN SORTING SIGNALS; PROTEIN TRANSPORT; PROTEINS; RECOMBINANT PROTEINS; SECRETORY PATHWAY;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; EUKARYOTA;

EID: 80052432622     PISSN: 13675435     EISSN: 14765535     Source Type: Journal    
DOI: 10.1007/s10295-011-0944-5     Document Type: Article

References (23)

1. JL Arolas J Lorenzo A Rovira J Castellà FX Aviles CP Sommerhoff 2005 A carboxypeptidase inhibitor from the tick Rhipicephalus bursa: isolation, cDNA cloning, recombinant expression, and characterization J Biol Chem 280 5 3441 3448 15561703 10.1074/jbc.M411086200 1:CAS:528:DC%2BD2MXovVGitg%3D%3D (Pubitemid 40223808)
2. JL Arolas J Vendrell FX Aviles LD Fricker 2007 Metallocarboxypeptidases: emerging drug targets in biomedicine Curr Pharm Des 13 4 349 366 17311554 10.2174/138161207780162980 1:CAS:528:DC%2BD2sXjvVyltro%3D (Pubitemid 46477758)
3. JM Betton D Boscus D Missiakas S Raina M Hofnung 1996 Probing the structural role of an alpha beta loop of maltose-binding protein by mutagenesis: heat-shock induction by loop variants of the maltose-binding protein that form periplasmic inclusion bodies J Mol Biol 262 2 140 150 8831785 10.1006/jmbi.1996.0504 1:CAS:528:DyaK28XmtVKmsLo%3D (Pubitemid 26326968)
4. JH Choi SY Lee 2004 Secretory and extracellular production of recombinant proteins using Escherichia coli Appl Microbiol Biotechnol 64 625 635 14966662 10.1007/s00253-004-1559-9 1:CAS:528:DC%2BD2cXjs1eiu7Y%3D (Pubitemid 38822625)
5. A de Marco 2009 Strategies for successful recombinant expression of disulfide bond-dependent proteins in Escherichia coli Microb Cell Fact 8 26 19442264 10.1186/1475-2859-8-26
6. O Durany P Bassett AM Weiss RM Cranenburgh P Ferrer J López-Santín C de Mas JA Hanak 2005 Production of fuculose-1-phosphate aldolase using operator-repressor titration for plasmid maintenance in high cell density Escherichia coli fermentations Biotechnol Bioeng 91 4 460 467 15959893 10.1002/bit.20527 1:CAS:528:DC%2BD2MXnt1ynsLs%3D (Pubitemid 41099031)
7. O Durany C de Mas J López-Santín 2004 Fed-batch production of recombinant fuculose-1-phosphate aldolase in E. coli Process Biochem 40 707 716 10.1016/j.procbio.2004.01.058
8. Fernández D, Pallarès I, Vendrell J, Avilés FX (2010) Progress in metallocarboxypeptidases and their small molecular weight inhibitors. Biochimie (in press). doi: 10.1016/j.biochi.2010.05.002
9. G Georgiou L Segatori 2005 Preparative expression of secreted proteins in bacteria: status report and future prospects Curr Opin Biotechnol 16 5 538 545 16095898 10.1016/j.copbio.2005.07.008 1:CAS:528:DC%2BD2MXhtVKjsLnP (Pubitemid 41393835)
10. LM Guzman D Belin MJ Carson J Beckwith 1995 Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter J Bacteriol 177 14 4121 4130 7608087 1:CAS:528:DyaK2MXmvV2gtL8%3D
11. FJ Mergulhão GA Monteiro 2007 Analysis of factors affecting the periplasmic production of recombinant proteins in Escherichia coli J Microbiol Biotechnol 17 8 1236 1241 18051590 (Pubitemid 47377388)
12. FJ Mergulhão GA Monteiro G Larsson M Bostrom A Farewell T Nyström JM Cabral MA Taipa 2003 Evaluation of inducible promoters on the secretion of a ZZ proinsulin fusion protein in Escherichia coli Biotechnol Appl Biochem 38 87 93 12740005 10.1042/BA20030043 (Pubitemid 36976423)
13. F Mergulhão G Monteiro G Larsson A Sanden A Farewell T Nystrom JM Cabral M Taipa 2003 Medium and copy number effects on the secretion of human proinsulin in Escherichia coli using the universal stress promoters uspA and uspB Appl Microbiol Biotechnol 61 495 501 12764564 (Pubitemid 36751012)
14. FJ Mergulhão DK Summers GA Monteiro 2005 Recombinant protein secretion in Escherichia coli Biotechnol Adv 23 3 177 202 15763404 10.1016/j.biotechadv.2004.11.003 (Pubitemid 40335946)
15. MA Molina FX Aviles E Querol 1992 Expression of a synthetic gene encoding potato carboxypeptidase inhibitor using a bacterial secretion vector Gene 116 129 138 1634110 10.1016/0378-1119(92)90508-M 1:CAS:528:DyaK3sXltlanurY%3D
16. JM Puertas JM Betton 2009 Engineering an efficient secretion of leech carboxypeptidase inhibitor in Escherichia coli Microb Cell Fact 29 57 10.1186/1475-2859-8-57
17. JM Puertas B Nannenga KT Dornfeld JM Betton F Baneyx 2010 Enhancing the secretory yields of leech carboxypeptidase inhibitor in Escherichia coli: influence of trigger factor and signal recognition particle Protein Exp Purif 74 1 122 128 10.1016/j.pep.2010.06.008 1:CAS:528:DC%2BC3cXhtVKhu73E
18. JM Puertas J Ruiz M Rodriguez de la Vega J Lorenzo G Caminal G González 2010 Influence of specific growth rate over the secretory expression of recombinant potato carboxypeptidase inhibitor in fed-batch cultures of Escherichia coli Proc Bio 45 1334 1341 10.1016/j.procbio.2010.04.024 1:CAS:528:DC%2BC3cXosV2gsLY%3D
19. Reverter D, Vendrell J, Canals F, Horstmann J, Aviles FX, Fritz H, Sommerhoff CP (1998) A carboxypeptidase inhibitor from the medical leech Hirudo medicinalis. Isolation, sequence analysis, cDNA cloning, recombinant expression, and characterization. J Biol Chem 273(49):32927-32933 (Pubitemid 28557685)
20. CF Schierle M Berkmen D Huber C Kumamoto D Boyd J Beckwith 2003 The DsbA signal sequence directs efficient, cotranslational export of passenger proteins to the Escherichia coli periplasm via the signal recognition particle pathway J Bacteriol 185 19 5706 5713 13129941 10.1128/JB.185.19.5706-5713.2003 1:CAS:528:DC%2BD3sXnslGlt7s%3D (Pubitemid 37176482)
21. A Shokri AM Sandén G Larsson 2003 Cell and process design for targeting of recombinant protein into the culture medium of Escherichia coli Appl Microbiol Biotechnol 60 6 654 664 12664143 1:CAS:528:DC%2BD3sXitlOqtr8%3D (Pubitemid 36286671)
22. L Vidal P Ferrer G Alvaro MD Benaiges G Caminal 2005 Influence of induction and operation mode on recombinant rhamnulose 1-phosphate aldolase production by Escherichia coli using the T5 promoter J Biotechnol 118 1 75 87 15908029 10.1016/j.jbiotec.2005.02.012 1:CAS:528:DC%2BD2MXlt1GitLY%3D (Pubitemid 40814536)
23. EW Wan F Baneyx 1998 TolAIII co-overexpression facilitates the recovery of periplasmic recombinant proteins into the growth medium of Escherichia coli Protein Expres Purif 14 13 22 10.1006/prep.1998.0941 1:CAS:528: DyaK1cXmslWksLY%3D (Pubitemid 28476575)