Increased structural flexibility at the active site of a fluorophore-conjugated β-lactamase distinctively impacts its binding toward diverse cephalosporin antibiotics

Journal of Biological Chemistry

Volumn 286, Issue 36, 2011, Pages 31771-31780

  Wong, Wai Ting ^a   Chan, Kwok Chu ^a   So, Pui Kin ^a   Yap, Hong Kin ^a   Chung, Wai Hong ^a   Leung, Yun Chung ^a   Wong, Kwok Yin ^a   Zhao, Yanxiang ^a  

^a HONG KONG POLYTECHNIC UNIVERSITY   (Hong Kong)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIVE SITE; BINDING KINETICS; BIOSENSING SYSTEMS; CEPHALOSPORIN ANTIBIOTICS; CONFORMATIONAL CHANGE; ENVIRONMENT-SENSITIVE; EVOLUTIONARY STRATEGIES; FLUORESCENCE SIGNALS; HYDROLYTIC ACTIVITIES; LACTAMASES; MOLECULAR EVOLUTION; SIGNIFICANT IMPACTS; STERIC HINDRANCES; STRUCTURAL CHANGE; STRUCTURAL FLEXIBILITIES; STRUCTURAL INFORMATION; STRUCTURAL STUDIES; SUBSTRATE PROFILE; SUBSTRATE-BINDING SITES;

ANTIBIOTICS; ENZYME KINETICS; EVOLUTIONARY ALGORITHMS; FLUORESCENCE; FLUOROPHORES; KINETICS; MOLECULAR BIOLOGY; SEWAGE TREATMENT PLANTS; STRUCTURAL DYNAMICS;

SUBSTRATES;

BETA LACTAMASE; CEFALORIDINE; CEFALOTIN; CEFOTAXIME; CEFOXITIN; CEFTAZIDIME; CEFTRIAXONE; CEFUROXIME; CEPHALOSPORIN DERIVATIVE; FLUORESCENT DYE; PENICILLIN G;

ARTICLE; BINDING KINETICS; BINDING SITE; CONFORMATIONAL TRANSITION; DRUG DESIGN; DRUG PROTEIN BINDING; ELECTROSPRAY MASS SPECTROMETRY; ENZYME ACTIVE SITE; ENZYME BINDING; ENZYME KINETICS; ENZYME STABILITY; ENZYME STRUCTURE; ENZYME SUBSTRATE; FLUORESCENCE ANALYSIS; GENETIC ENGINEERING; GENETIC PROCEDURES; MUTATIONAL ANALYSIS; PRIORITY JOURNAL;

BETA-LACTAMASES; BINDING SITES; CATALYTIC DOMAIN; CEPHALOSPORINS; FLUORESCENT DYES; KINETICS; MOLECULAR CONFORMATION; MOLECULAR PROBES; MUTAGENESIS, SITE-DIRECTED; PLIABILITY; PROTEIN ENGINEERING; SUBSTRATE SPECIFICITY;

EID: 80052398961     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M110.198895     Document Type: Article

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