Activity in vivo of anti-trypanosoma cruzi compounds selected from a high throughput screening

PLoS Neglected Tropical Diseases

Volumn 5, Issue 8, 2011, Pages

  Andriani, Grasiella ^a   Chessler, Anne Danielle C ^b   Courtemanche, Gilles ^c   Burleigh, Barbara A ^b   Rodriguez, Ana ^a  

^a NEW YORK UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b HARVARD SCHOOL OF PUBLIC HEALTH   (United States)

^c SANOFI   (France)

Author keywords

[No Author keywords available]

Indexed keywords

ANTITRYPANOSOMAL AGENT; BENZNIDAZOLE; ANTIPROTOZOAL AGENT;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANTIPROTOZOAL ACTIVITY; ARTICLE; CHAGAS DISEASE; CONTROLLED STUDY; DRUG CYTOTOXICITY; DRUG PURIFICATION; DRUG STRUCTURE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HIGH THROUGHPUT SCREENING; HUMAN; HUMAN CELL; IC 50; IN VITRO STUDY; IN VIVO STUDY; MASS SPECTROMETRY; MOUSE; NONHUMAN; TOXICITY TESTING; TRYPANOSOMA CRUZI; ANIMAL; BAGG ALBINO MOUSE; BIOASSAY; CHEMISTRY; DISEASE MODEL; DRUG EFFECT; DRUG SENSITIVITY; ISOLATION AND PURIFICATION; METHODOLOGY;

ANIMALS; ANTIPROTOZOAL AGENTS; BIOLOGICAL ASSAY; CHAGAS DISEASE; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; DISEASE MODELS, ANIMAL; HIGH-THROUGHPUT SCREENING ASSAYS; INHIBITORY CONCENTRATION 50; MASS SPECTROMETRY; MICE; MICE, INBRED BALB C; PARASITIC SENSITIVITY TESTS; TRYPANOSOMA CRUZI;

EID: 80052392943     PISSN: 19352727     EISSN: 19352735     Source Type: Journal    
DOI: 10.1371/journal.pntd.0001298     Document Type: Article

References (15)

1. Rassi A Jr, Rassi A, Marin-Neto JA, (2010) Chagas disease. Lancet 375: 1388-1402.
2. Urbina JA, Docampo R, (2003) Specific chemotherapy of Chagas disease: controversies and advances. Trends in parasitology 19: 495-501.
3. Viotti R, Vigliano C, Lococo B, Bertocchi G, Petti M, et al. (2006) Long-term cardiac outcomes of treating chronic Chagas disease with benznidazole versus no treatment: a nonrandomized trial. Annals of internal medicine 144: 724-734.
4. Olivieri BP, Molina JT, de Castro SL, Pereira MC, Calvet CM, et al. (2010) A comparative study of posaconazole and benznidazole in the prevention of heart damage and promotion of trypanocidal immune response in a murine model of Chagas disease. International journal of antimicrobial agents 36: 79-83.
5. Pinazo MJ, Espinosa G, Gallego M, Lopez-Chejade PL, Urbina JA, et al. (2010) Successful treatment with posaconazole of a patient with chronic Chagas disease and systemic lupus erythematosus. The American journal of tropical medicine and hygiene 82: 583-587.
6. Phatak SS, Stephan CC, Cavasotto CN, (2009) High-throughput and in silico screenings in drug discovery. Expert Opin Drug Discov 4: 947-959.
7. Miraglia L, King F, Damoiseaux R, (2011) Seeing the Light: Luminescent Reporter Gene Assays. Comb Chem High Throughput Screen.
8. Buckner FS, Verlinde CL, La Flamme AC, Van Voorhis WC, (1996) Efficient technique for screening drugs for activity against Trypanosoma cruzi using parasites expressing beta-galactosidase. Antimicrobial agents and chemotherapy 40: 2592-2597.
9. Canavaci AM, Bustamante JM, Padilla AM, Perez Brandan CM, Simpson LJ, et al. (2010) In vitro and in vivo high-throughput assays for the testing of anti-Trypanosoma cruzi compounds. PLoS neglected tropical diseases 4: e740.
10. Hyland KV, Asfaw SH, Olson CL, Daniels MD, Engman DM, (2008) Bioluminescent imaging of Trypanosoma cruzi infection. International journal for parasitology 38: 1391-1400.
11. Ashutosh, Gupta S, Ramesh, Sundar S, Goyal N, (2005) Use of Leishmania donovani field isolates expressing the luciferase reporter gene in in vitro drug screening. Antimicrobial agents and chemotherapy 49: 3776-3783.
12. Lang T, Goyard S, Lebastard M, Milon G, (2005) Bioluminescent Leishmania expressing luciferase for rapid and high throughput screening of drugs acting on amastigote-harbouring macrophages and for quantitative real-time monitoring of parasitism features in living mice. Cellular microbiology 7: 383-392.
13. Vazquez MP, Levin MJ, (1999) Functional analysis of the intergenic regions of TcP2beta gene loci allowed the construction of an improved Trypanosoma cruzi expression vector. Gene 239: 217-225.
14. Martinez-Calvillo S, Lopez I, Hernandez R, (1997) pRIBOTEX expression vector: a pTEX derivative for a rapid selection of Trypanosoma cruzi transfectants. Gene 199: 71-76.
15. Stiles B, Kierszenbaum F, (1986) An improved procedure for the purification of Trypanosoma cruzi (Chagas, 1909) metacyclics from the insect vector. J Protozool 33: 132-134.