Metabolic labeling of model organisms using heavy nitrogen (15N)

Methods in Molecular Biology

Volumn 753, Issue , 2011, Pages 29-42

  Gouw, Joost W ^a   Tops, Bastiaan B J ^b   Krijgsveld, Jeroen ^c  

^a UNIVERSITY OF BRITISH COLUMBIA   (Canada)

^b UTRECHT UNIVERSITY   (Netherlands)

^c EUROPEAN MOLECULAR BIOLOGY LABORATORY   (Germany)

Author keywords

C. elegans.; Drosophila; Mass spectrometry; Model organism; Nitrogen; Protein quantitation; Proteomics; Stable isotope labeling

Indexed keywords

NITROGEN; PEPTIDE; PROTEIN;

ANIMAL; ARTICLE; CAENORHABDITIS ELEGANS; CHEMISTRY; DROSOPHILA MELANOGASTER; ESCHERICHIA COLI; FEMALE; ISOTOPE LABELING; MALE; MASS SPECTROMETRY; METABOLISM; METHODOLOGY; PROTEOMICS; SACCHAROMYCES CEREVISIAE;

ANIMALS; CAENORHABDITIS ELEGANS; DROSOPHILA MELANOGASTER; ESCHERICHIA COLI; FEMALE; ISOTOPE LABELING; MALE; MASS SPECTROMETRY; NITROGEN; PEPTIDES; PROTEINS; PROTEOMICS; SACCHAROMYCES CEREVISIAE;

EID: 80052380931     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-148-2_2     Document Type: Article

References (20)

1. Ong, S. E., and Mann, M. (2005) Mass spectrometry-based proteomics turns quantitative, Nat Chem Biol 1, 252-262.
2. Ross, P. L., Huang, Y. N., Marchese, J. N., Williamson, B., Parker, K., Hattan, S., Khainovski, N., Pillai, S., Dey, S., Daniels, S., Purkayastha, S., Juhasz, P., Martin, S., Bartlet-Jones, M., He, F., Jacobson, A., and Pappin, D. J. (2004) Multiplexed protein quantitation in Saccharomyces cerevisiae using amine-reactive isobaric tagging reagents, Mol Cell Proteomics 3, 1154-1169.
3. Gygi, S. P., Rist, B., Gerber, S. A., Turecek, F., Gelb, M. H., and Aebersold, R. (1999) Quantitative analysis of complex protein mixtures using isotope-coded affinity tags, Nat Biotechnol 17, 994-999.
4. Boersema, P. J., Raijmakers, R., Lemeer, S., Mohammed, S., and Heck, A. J. (2009) Multiplex peptide stable isotope dimethyl labeling for quantitative proteomics, Nat Protoc 4, 484-494.
5. Everley, P. A., Bakalarski, C. E., Elias, J. E., Waghorne, C. G., Beausoleil, S. A., Gerber, S. A., Faherty, B. K., Zetter, B. R., and Gygi, S. P. (2006) Enhanced analysis of metastatic prostate cancer using stable isotopes and high mass accuracy instrumentation, J Proteome Res 5, 1224-1231.
6. Oda, Y., Huang, K., Cross, F. R., Cowburn, D., and Chait, B. T. (1999) Accurate quantitation of protein expression and site-specific phosphorylation, Proc Natl Acad Sci USA 96, 6591-6596.
7. Krijgsveld, J., Ketting, R. F., Mahmoudi, T., Johansen, J., Artal-Sanz, M., Verrijzer, C. P., Plasterk, R. H., and Heck, A. J. (2003) Metabolic labeling of C. elegans and D. melanogaster for quantitative proteomics, Nat Biotechnol 21, 927-931.
8. Dong, M. Q., Venable, J. D., Au, N., Xu, T., Park, S. K., Cociorva, D., Johnson, J. R., Dillin, A., and Yates, J. R., 3rd. (2007) Quantitative mass spectrometry identifies insulin signaling targets in C. elegans, Science 317, 660-663.
9. Findlay, G. D., Yi, X., Maccoss, M. J., and Swanson, W. J. (2008) Proteomics reveals novel Drosophila seminal fluid proteins transferred at mating, PLoS Biol 6, e178.
10. Kim, J. K., Harada, K., Bamba, T., Fukusaki, E.-i., and Kobayashi, A. (2005) Stable isotope dilution-based accurate comparative quantification of nitrogen-containing metabolites in Arabidopsis thaliana T87 Cells using in vivo 15 N-isotope enrichment, Biosci Biotechnol Biochem 69, 1331-1340.
11. Wu, C. C., MacCoss, M. J., Howell, K. E., Matthews, D. E., and Yates, J. R., 3rd. (2004) Metabolic labeling of mammalian organisms with stable isotopes for quantitative proteomic analysis, Anal Chem 76, 4951-4959.
12. Gouw, J. W., Pinkse, M. W., Vos, H. R., Moshkin, Y. M., Verrijzer, C. P., Heck, A. J. R., and Krijgsveld, J. (2009) In vivo stable isotope labeling of fruit flies reveals posttranscriptional regulation in the maternal-to-zygotic transition, Mol Cell Proteomics 8, 1566-1578.
13. 15N isotope labeling, J Proteome Res 9, 341-351.
14. (n): Technical design and applications to peptide and protein analysis, J Sep Sci 25, 557-568.
15. 15N-labeled proteins in comparative proteomics, Anal Chem 80, 7796-7803.
16. Motoyama, A., and Yates, J. R., 3rd. (2008) Multidimensional LC separations in shotgun proteomics, Anal Chem 80, 7187-7193.
17. Qian, W. J., Jacobs, J. M., Liu, T., Camp, D. G., 2nd, and Smith, R. D. (2006) Advances and challenges in liquid chromatographymass spectrometry-based proteomics profiling for clinical applications, Mol Cell Proteomics 5, 1727-1744.
18. Mortensen, P., Gouw, J. W., Olsen, J. V., Ong, S. E., Rigbolt, K. T., Bunkenborg, J., Cox, J., Foster, L. J., Heck, A. J., Blagoev, B., Andersen, J. S., and Mann, M. (2010) MSQuant, an open source Platform for mass spectrometry-based quantitative proteomics, J Proteome Res 9, 393-403.
19. Park, S. K., Venable, J. D., Xu, T., and Yates, J. R., 3rd. (2008) A quantitative analysis software tool for mass spectrometry-based proteomics, Nat Methods 5, 319-322.
20. Rappsilber, J., Ishihama, Y., and Mann, M. (2003) Stop and go extraction tips for matrix-assisted laser desorption/ionization, nanoelectrospray, and LC/MS sample pretreatment in proteomics, Anal Chem 75, 663-670.