Quantitative Proteome Analysis Using Isobaric Peptide Termini Labeling (IPTL)

Methods in Molecular Biology

Volumn 753, Issue , 2011, Pages 65-76

  Arntzen, Magnus Ø ^a,b   Koehler, Christian J ^a   Treumann, Achim ^a   Thiede, Bernd ^c  

^a UNIVERSITY OF OSLO   (Norway)

^b NORWEGIAN UNIVERSITY OF LIFE SCIENCES   (Norway)

^c Devonshire Building   (United Kingdom)

Author keywords

Chemical labeling; IPTL; Isobaric labeling; IsobariQ; iTRAQ; Mass spectrometry; Quantitative proteomics; TMT

Indexed keywords

PEPTIDE; PROTEOME;

ARTICLE; CHEMISTRY; GENETICS; ISOTOPE LABELING; METABOLISM; METHODOLOGY; PROTEOMICS; TANDEM MASS SPECTROMETRY;

ISOTOPE LABELING; PEPTIDES; PROTEOME; PROTEOMICS; TANDEM MASS SPECTROMETRY;

EID: 80052364820     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-148-2_5     Document Type: Article

References (7)

1. Ong, S. E., and Mann, M. (2005) Mass spectrometry-based proteomics turns quantitative. Nat. Chem. Biol. 1, 252-262.
2. Thompson, A., Schafer, J., Kuhn, K., Kienle, S., Schwarz, J., Schmidt, G., et al. (2003) Tandem mass tags: a novel quantification strategy for comparative analysis of complex protein mixtures by MS/MS. Anal. Chem. 75, 1895-1904.
3. Ross, P. L., Huang, Y. N., Marchese, J. N., Williamson, B., Parker, K., Hattan, S., et al. (2004) Multiplexed protein quantitation in Saccharomyces cerevisiae using aminereactive isobaric tagging reagents. Mol. Cell Proteomics 3, 1154-1169.
4. Choe, L., D'Ascenzo, M., Relkin, D., Ross, P., Williamson, B., Guertin, S. et al. (2007) 8-plex quantitation of changes in cerebrospinal fluid protein expression in subjects undergoing intravenous immunoglobulin treatment of Alzheimer's disease. Proteomics 7, 3651-3660.
5. Koehler, C. J., Strozynski, M., Kozielski, F., Treumann, A., and Thiede, B. (2009) Isobaric peptide termini labeling for MS/MS-based quantitative proteomics. J. Proteome. Res. 8, 4333-4341.
6. Webster, J., and Oxley, D. (2005) Peptide mass fingerprinting: protein identification using MALDI-TOF mass spectrometry. Methods Mol. Biol. 310, 227-240.
7. Arntzen, M.Ø., Koehler, C.J., Barsnes, F.S., Berven, F.S., Treumann, A., and Thiede, B. (2011) IsobariQ: software for isobaric quantitative proteomics using IPTL, iTRAQ, and TMT. J. Proteome Res. 10, 913-920.