Transcription factor proteomics: Identification by a novel gel mobility shift-three-dimensional electrophoresis method coupled with southwestern blot and high-performance liquid chromatography-electrospray-mass spectrometry analysis

Journal of Chromatography A

Volumn 1218, Issue 39, 2011, Pages 7003-7015

  Jiang, Daifeng ^a   Jia, Yinshan ^a   Jarrett, Harry W ^a  

^a UNIVERSITY OF TEXAS AT SAN ANTONIO   (United States)

Author keywords

AP1; C EBP; Electrophoresis; Proteomics; Transcription factor

Indexed keywords

AP1; BINDING ACTIVITIES; C/EBP; CRUDE EXTRACT; DNA-BINDING; DNA-BINDING PROTEIN; DNA-PROTEIN COMPLEXES; ELECTROPHORETIC MOBILITY SHIFT ASSAY; FUSION PROTEINS; GEL MOBILITY; HEK-293; HEK293 CELLS; HUMAN EMBRYONIC KIDNEY CELLS; NEW STRATEGY; NUCLEAR EXTRACTS; PROTEOMICS; PVDF MEMBRANE; REGULATORY MECHANISM; SDS-PAGE; SUPERSHIFT ASSAYS; TRANSCRIPTIONAL COMPLEXES; TRYPSIN DIGESTION; TWO-DIMENSIONAL GEL ELECTROPHORESIS; WESTERN BLOTTING; WESTERN-BLOT ANALYSIS;

CELL CULTURE; COMPLEXATION; DETERGENTS; DNA; GELS; GENES; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; MOLECULAR BIOLOGY; PURIFICATION; RADIOGRAPHY; TRANSCRIPTION; TRANSCRIPTION FACTORS;

ELECTROPHORETIC MOBILITY;

ACTIVATING TRANSCRIPTION FACTOR 1; ACTIVATING TRANSCRIPTION FACTOR 2; CYCLIC AMP RESPONSIVE ELEMENT BINDING PROTEIN; DNA BINDING PROTEIN; HYBRID PROTEIN; PROTEIN C FOS; PROTEIN C JUN; PROTEOME; TRANSCRIPTION FACTOR; TRANSCRIPTION FACTOR JUND; TRYPSIN;

AMINO ACID SEQUENCE; ARTICLE; AUTORADIOGRAPHY; CONTROLLED STUDY; ELECTROSPRAY MASS SPECTROMETRY; ENZYME PURIFICATION; GEL ELECTROPHORESIS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; HUMAN CELL; NUCLEOTIDE SEQUENCE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DNA BINDING; PROTEIN FUNCTION; PROTEIN LOCALIZATION; PROTEOMICS; SOUTHWESTERN BLOTTING; THREE DIMENSIONAL GEL ELECTROPHORESIS; TWO DIMENSIONAL GEL ELECTROPHORESIS; WESTERN BLOTTING;

AMINO ACID SEQUENCE; BLOTTING, SOUTHWESTERN; CCAAT-ENHANCER-BINDING PROTEINS; CELL NUCLEUS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; DNA-BINDING PROTEINS; ELECTROPHORETIC MOBILITY SHIFT ASSAY; HEK293 CELLS; HUMANS; MOLECULAR SEQUENCE DATA; PROTEOMICS; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; TRANSCRIPTION FACTOR AP-1; TRANSCRIPTION FACTORS;

BACTERIA (MICROORGANISMS);

EID: 80052317899     PISSN: 00219673     EISSN: 18733778     Source Type: Journal    
DOI: 10.1016/j.chroma.2011.08.023     Document Type: Article

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