Validation and standardization of IS900 and F57 real-time quantitative PCR assays for the specific detection and quantification of Mycobacterium avium subsp. paratuberculosis

Canadian Journal of Microbiology

Volumn 57, Issue 5, 2011, Pages 347-354

  Sidoti, Francesca ^a   Banche, Giuliana ^a   Astegiano, Sara ^a   Allizond, Valeria ^a   Cuffini, Anna Maria ^a   Bergallo, Massimiliano ^a  

^a UNIVERSITY OF TURIN   (Italy)

Author keywords

IS900 and F57 sequences; Mycobacterium avium subsp. paratuberculosis; Real time qPCR assay

Indexed keywords

CAUSATIVE AGENTS; CROHN'S DISEASE; EXPERIMENTAL STUDIES; IN-SILICO; INSERTION SEQUENCES; IS900 AND F57 SEQUENCES; JOHNE'S DISEASE; MYCOBACTERIAL; MYCOBACTERIUM AVIUM SUBSP. PARATUBERCULOSIS; PASTEURIZED MILK; RAPID DETECTION; REAL-TIME QPCR ASSAY; REAL-TIME QUANTITATIVE PCR; REPRODUCIBILITIES; SPECIFIC DETECTION;

ASSAYS; STANDARDIZATION;

POLYMERASE CHAIN REACTION;

SILICON;

ACCURACY ASSESSMENT; BACTERIAL DISEASE; BACTERIUM; BIOASSAY; DETECTION METHOD; DISEASE TRANSMISSION; MILK; MODEL VALIDATION; POLYMERASE CHAIN REACTION; PRECISION; PUBLIC HEALTH; QUANTITATIVE ANALYSIS; REAL TIME;

ACCURACY; ARTICLE; CROHN DISEASE; EXPERIMENTAL STUDY; GENE INSERTION SEQUENCE; GENE SEQUENCE; HUMAN; MILK; MYCOBACTERIUM AVIUM; NONHUMAN; PARATUBERCULOSIS; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY; STANDARDIZATION;

ADULT; AGED; CROHN DISEASE; DNA TRANSPOSABLE ELEMENTS; DNA, BACTERIAL; FEMALE; HUMANS; MALE; MIDDLE AGED; MYCOBACTERIUM AVIUM SUBSP. PARATUBERCULOSIS; PARATUBERCULOSIS; REAL-TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY;

MYCOBACTERIUM AVIUM SUBSP. PARATUBERCULOSIS;

EID: 80052275459     PISSN: 00084166     EISSN: 14803275     Source Type: Journal    
DOI: 10.1139/w11-022     Document Type: Article

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