Clinical significance of methylation of plasma Ras-association domain family 1A gene in the molecular diagnosis of hepatocellular carcinoma

Tumor

Volumn 31, Issue 8, 2011, Pages 742-747

  Fei, Bo Jian ^a   Huang, Zhao Hui ^a   Hua, Dong ^a   Hu, Yu ^a   Cheng, Zhi Hong ^a   Yu, Jian ^b  

^a SOOCHOW UNIVERSITY   (China)

^b SHANGHAI CANCER INSTITUTE   (China)

Author keywords

Carcinoma, hepatocellular; DNA methylation; Molecular diagnostic techniques; Plasma; Polymerase chain reaction; Ras association domain family 1A; Restriction enzyme

Indexed keywords

ALPHA FETOPROTEIN; GENOMIC DNA; RAS ASSOCIATION DOMAIN FAMILY PROTEIN 1A;

ARTICLE; BLOOD SAMPLING; CHRONIC HEPATITIS; CLINICAL ASSESSMENT; CLINICAL EFFECTIVENESS; CONTROLLED STUDY; DIAGNOSTIC TEST ACCURACY STUDY; DIAGNOSTIC VALUE; DISEASE ASSOCIATION; DNA METHYLATION; HUMAN; HUMAN TISSUE; LIVER; LIVER CELL CARCINOMA; LIVER CIRRHOSIS; METHYLATION SENSITIVE RESTRICTION ENZYME BASED QUANTITATIVE POLYMERASE CHAIN REACTION; MOLECULAR DIAGNOSIS; NON INVASIVE PROCEDURE; PLASMA; POLYMERASE CHAIN REACTION; QUANTITATIVE ANALYSIS;

EID: 80052258549     PISSN: 10007431     EISSN: None     Source Type: Journal    
DOI: 10.3781/j.issn.1000-7431.2011.08.012     Document Type: Article

References (14)

1. MCCABE M T, BRANDES J C, VERTINO P M. Cancer DNA methylation: molecular mechanisms and clinical implications[J]. Clin Cancer Res , 2009, 15(12): 3927-3937.
2. BUTT A N, SWAMINATHAN R. Overview of circulating nucleic acids in plasma/serum[J]. Ann N Y Acad Sci , 2008, 1137: 236-242.
3. NISHIDA N, NAGASAKA T, NISHIMURA T, et al . Aberrant methylation of multiple tumor suppressor genes in aging liver, chronic hepatitis, and hepatocellular carcinoma[J]. Hepatology , 2008, 47(3): 908-918.
4. CHAN K C, LAI P B, MOK T S, et al . Quantitative analysis of circulating methylated DNA as a biomarker for hepatocellular carcinoma[J]. Clin Chem, 2008, 54(9): 1528-1536.
5. ZHANG Y J, WU H C, SHEN J, et al . Predicting hepatocellular carcinoma by detection of aberrant promoter methylation in serum DNA[J]. Clin Cancer Res , 2007, 13(8): 2378-2384.
6. HUANG Z H, LI L H, HUA D. Quantitative analysis of plasma circulating DNA at diagnosis and during follow-up of breast cancer patients[J]. Cancer Lett , 2006, 243(1): 64-70.
7. HERMAN J G, GRAFF J R, MYÖHÄNEN S, et al . Methylation-specific PCR: a novel PCR assay for methylation status of CpG islands[J]. Proc Natl Acad Sci U S A , 1996, 93(18): 9821-9826.
8. MELNIKOV A A, GARTENHAUS R B, LEVENSON A S, et al . MSRE-PCR for analysis of gene-specific DNA methylation[J]. Nucleic Acids Res , 2005, 33(10): e93.
9. HU L, CHEN G, YU H, et al . Clinicopathological significance of RASSF1A reduced expression and hypermethylation in hepatocellular carcinoma[J]. Hepatol Int , 2010, 4(1): 423-432.