Establishment of clinically compliant human embryonic stem cells in an autologous feeder-free system

Tissue Engineering - Part C: Methods

Volumn 17, Issue 9, 2011, Pages 927-937

  Fu, Xin ^a   Toh, Wei Seong ^a   Liu, Hua ^a   Lu, Kai ^a   Li, Mingming ^a   Cao, Tong ^a  

^a NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

Author keywords

[No Author keywords available]

Indexed keywords

CELL CULTURE; FLOW CYTOMETRY; GENE EXPRESSION; MAMMALS; STEM CELLS;

CULTURE SYSTEMS; EMBRYOID BODY; EXTRACELLULAR MATRICES; FEEDER CELLS; FREEZE-THAW; HUMAN EMBRYONIC STEM CELLS; IN-VITRO; IN-VIVO; LAMININ; LONG-TERM CULTURE; MARKER GENES; MOUSE EMBRYONIC FIBROBLASTS; PLURIPOTENCY;

FEEDING;

ANIMALIA;

ANIMAL; ARTICLE; CELL DIFFERENTIATION; CELL LINE; CELL PROLIFERATION; CELL SHAPE; CULTURE TECHNIQUE; CYTOLOGY; EMBRYOID BODY; EMBRYONIC STEM CELL; EXTRACELLULAR MATRIX; FEEDER CELL; FIBROBLAST; FLOW CYTOMETRY; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION; GENETICS; HUMAN; KARYOTYPING; METABOLISM; METHODOLOGY; MOUSE; PLURIPOTENT STEM CELL; REAL TIME POLYMERASE CHAIN REACTION;

ANIMALS; CELL CULTURE TECHNIQUES; CELL DIFFERENTIATION; CELL LINE; CELL PROLIFERATION; CELL SHAPE; EMBRYOID BODIES; EMBRYONIC STEM CELLS; EXTRACELLULAR MATRIX; FEEDER CELLS; FIBROBLASTS; FLOW CYTOMETRY; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION; HUMANS; KARYOTYPING; MICE; PLURIPOTENT STEM CELLS; REAL-TIME POLYMERASE CHAIN REACTION;

EID: 80052243951     PISSN: 19373384     EISSN: 19373392     Source Type: Journal    
DOI: 10.1089/ten.tec.2010.0735     Document Type: Article

References (120)

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70. Lu, J., Hou, R., Booth, C.J., Yang, S.H., and Snyder, M. Defined culture conditions of human embryonic stem cells. Proc Natl Acad Sci USA 103, 5688, 2006.
71. Sjogren-Jansson, E., Zetterstrom, M., Moya, K., Lindqvist, J., Strehl, R., and Eriksson, P.S. Large-scale propagation of four undifferentiated human embryonic stem cell lines in a feeder-free culture system. Dev Dyn 233, 1304, 2005.
72. Vallier, L., Alexander, M., and Pedersen, R.A. Activin/Nodal and FGF pathways cooperate to maintain pluripotency of human embryonic stem cells. J Cell Sci 118, 4495, 2005.
73. Yao, S., Chen, S., Clark, J., Hao, E., Beattie, G.M., Hayek, A., et al. Long-term self-renewal and directed differentiation of human embryonic stem cells in chemically defined conditions. Proc Natl Acad Sci USA 103, 6907, 2006.
74. Ludwig, T.E., Levenstein, M.E., Jones, J.M., Berggren, W.T., Mitchen, E.R., Frane, J.L., et al. Derivation of human embryonic stem cells in defined conditions. Nat Biotechnol 24, 185, 2006.
75. Wang, L., Schulz, T.C., Sherrer, E.S., Dauphin, D.S., Shin, S., Nelson, A.M., et al. Self-renewal of human embryonic stem cells requires insulin-like growth factor-1 receptor and ERBB2 receptor signaling. Blood 110, 4111, 2007.
76. Kitajima, H., and Niwa, H. Clonal expansion of human pluripotent stem cells on gelatin-coated surface. Biochem Biophys Res Commun 396, 933, 2010.
77. Klimanskaya, I., Chung, Y., Meisner, L., Johnson, J., West, M.D., and Lanza, R. Human embryonic stem cells derived without feeder cells. Lancet 365, 1636, 2005.
78. Amit, M., Shariki, C., Margulets, V., and Itskovitz-Eldor, J. Feeder layer-and serum-free culture of human embryonic stem cells. Biol Reprod 70, 837, 2004.
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80. Beattie, G.M., Lopez, A.D., Bucay, N., Hinton, A., Firpo, M.T., King, C.C., et al. Activin A maintains pluripotency of human embryonic stem cells in the absence of feeder layers. Stem Cells 23, 489, 2005.
81. Li, Y., Powell, S., Brunette, E., Lebkowski, J., and Mandalam, R. Expansion of human embryonic stem cells in defined serum-free medium devoid of animal-derived products. Biotechnol Bioeng 91, 688, 2005.
82. Liu, Y., Song, Z., Zhao, Y., Qin, H., Cai, J., Zhang, H., et al. A novel chemical-defined medium with bFGF and N2B27 supplements supports undifferentiated growth in human embryonic stem cells. Biochem Biophys Res Commun 346, 131, 2006.
83. Akopian, V., Andrews, P.W., Beil, S., Benvenisty, N., Brehm, J., Christie, M., et al. Comparison of defined culture systems for feeder cell free propagation of human embryonic stem cells. In Vitro Cell Dev Biol Anim 46, 247, 2010.
84. Hakala, H., Rajala, K., Ojala, M., Panula, S., Areva, S., Kellomaki, M., et al. Comparison of biomaterials and extracellular matrices as a culture platform for multiple, independently derived human embryonic stem cell lines. Tissue Eng Part A 15, 1775, 2009.
85. Moore, H. The medium is the message. Nat Biotechnol 24, 160, 2006.
86. Meng, G., Liu, S., Li, X., Krawetz, R., and Rancourt, D.E. Extracellular matrix isolated from foreskin fibroblasts supports long-term xeno-free human embryonic stem cell culture. Stem Cells Dev 19, 547, 2010.
87. Fu, X., Toh, W.S., Liu, H., Lu, K., Li, M., Hande, M.P., et al. Autologous feeder cells from embryoid body outgrowth support the long-term growth of human embryonic stem cells more effectively than those from direct differentiation. Tissue Eng Part C Methods 16, 719, 2010.
88. Itskovitz-Eldor, J., Schuldiner, M., Karsenti, D., Eden, A., Yanuka, O., Amit, M., et al. Differentiation of human embryonic stem cells into embryoid bodies compromising the three embryonic germ layers. Mol Med 6, 88, 2000.
89. Toh, W.S., Yang, Z., Liu, H., Heng, B.C., Lee, E.H., and Cao, T. Effects of culture conditions and bone morphogenetic protein 2 on extent of chondrogenesis from human embryonic stem cells. Stem Cells 25, 950, 2007.
90. Fluckiger, A.C., Marcy, G., Marchand, M., Negre, D., Cosset, F.L., Mitalipov, S., et al. Cell cycle features of primate embryonic stem cells. Stem Cells 24, 547, 2006.
91. Yang, Z., Sui, L., Toh, W.S., Lee, E.H., and Cao, T. Stagedependent effect of TGF-beta1 on chondrogenic differentiation of human embryonic stem cells. Stem Cells Dev 18, 929, 2009.
92. Livak, K.J., and Schmittgen, T.D. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods 25, 402, 2001.
93. Toh, W.S., Guo, X.M., Choo, A.B., Lu, K., Lee, E.H., and Cao, T. Differentiation and enrichment of expandable chondrogenic cells from human embryonic stem cells in vitro. J Cell Mol Med 13, 3570, 2009.
94. Saylam, C., Ozdemir, N., Itil, I.M., Sendag, F., and Terek, M.C. Distribution of fibronectin, laminin and collagen type IV in the materno-fetal boundary zone of the developing mouse placenta. Experimental study. Arch Gynecol Obstet 266, 83, 2002.
95. Alberts, B., Johnson, A., Lewis, J., Raff, M., Roberts, K., and Walter, P. (eds.) The extracellular matrix of animals. In: Molecular Biology of The Cell, 4th edition. New York: Garland Science, 2002, pp. 1131-1195.
96. Amenta, P.S., Gay, S., Vaheri, A., and Martinez-Hernandez, A. The extracellular matrix is an integrated unit: ultrastructural localization of collagen types I, III, IV, V, VI, fibronectin, and laminin in human term placenta. Coll Relat Res 6, 125, 1986.
97. Beacham, D.A., Amatangelo, M.D., and Cukierman, E. Preparation of extracellular matrices produced by cultured and primary fibroblasts. Curr Protoc Cell Biol Chapter 10, Unit 10.9, 2007.
98. Neganova, I., Zhang, X., Atkinson, S., and Lako, M. Expression and functional analysis of G1 to S regulatory components reveals an important role for CDK2 in cell cycle regulation in human embryonic stem cells. Oncogene 28, 20, 2009.
99. Becker, K.A., Ghule, P.N., Therrien, J.A., Lian, J.B., Stein, J.L., van Wijnen, A.J., et al. Self-renewal of human embryonic stem cells is supported by a shortened G1 cell cycle phase. J Cell Physiol 209, 883, 2006.
100. Cukierman, E., Pankov, R., Stevens, D.R., and Yamada, K.M. Taking cell-matrix adhesions to the third dimension. Science 294, 1708, 2001.
101. Cobo, F., Stacey, G.N., Hunt, C., Cabrera, C., Nieto, A., Montes, R., et al. Microbiological control in stem cell banks: approaches to standardisation. Appl Microbiol Biotechnol 68, 456, 2005.
102. Martin, M.J., Muotri, A., Gage, F., and Varki, A. Human embryonic stem cells express an immunogenic nonhuman sialic acid. Nat Med 11, 228, 2005.
103. Rajala, K., Lindroos, B., Hussein, S.M., Lappalainen, R.S., Pekkanen-Mattila, M., Inzunza, J., et al. A defined and xenofree culture method enabling the establishment of clinicalgrade human embryonic, induced pluripotent and adipose stem cells. PLoS One 5, e10246, 2010.
104. Skottman, H., and Hovatta, O. Culture conditions for human embryonic stem cells. Reproduction 132, 691, 2006.
105. Guo, X., Hutcheon, A.E., Melotti, S.A., Zieske, J.D., Trinkaus-Randall, V., and Ruberti, J.W. Morphologic characterization of organized extracellular matrix deposition by ascorbic acid-stimulated human corneal fibroblasts. Invest Ophthalmol Vis Sci 48, 4050, 2007.
106. Sommer, F., Kobuch, K., Brandl, F., Wild, B., Framme, C., Weiser, B., et al. Ascorbic acid modulates proliferation and extracellular matrix accumulation of hyalocytes. Tissue Eng 13, 1281, 2007.
107. Lareu, R.R., Subramhanya, K.H., Peng, Y., Benny, P., Chen, C., Wang, Z., et al. Collagen matrix deposition is dramatically enhanced in vitro when crowded with charged macromolecules: the biological relevance of the excluded volume effect. FEBS Lett 581, 2709, 2007.
108. Lareu, R.R., Arsianti, I., Subramhanya, H.K., Yanxian, P., and Raghunath, M. In vitro enhancement of collagen matrix formation and crosslinking for applications in tissue engineering: a preliminary study. Tissue Eng 13, 385, 2007.
109. Hedman, K., Kurkinen, M., Alitalo, K., Vaheri, A., Johansson, S., and Hook, M. Isolation of the pericellular matrix of human fibroblast cultures. J Cell Biol 81, 83, 1979.
110. Picache, E.R., Hassanieh, L., Broek, D., and Schonthal, A.H. Inhibition of tumor cell growth by Triton X-100 through specific effects on cell-cycle-regulatory components. J Biomed Sci 11, 95, 2004.
111. Rotunda, A.M., Suzuki, H., Moy, R.L., and Kolodney, M.S. Detergent effects of sodium deoxycholate are a major feature of an injectable phosphatidylcholine formulation used for localized fat dissolution. Dermatol Surg 30, 1001, 2004.
112. Pakzad, M., Totonchi, M., Taei, A., Seifinejad, A., Hassani, S.N., and Baharvand, H. Presence of a ROCK inhibitor in extracellular matrix supports more undifferentiated growth of feeder-free human embryonic and induced pluripotent stem cells upon passaging. Stem Cell Rev 6, 96, 2010.
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