A comparison of methods for quantifying angiogenesis in the matrigel assay in vitro

Tissue Engineering - Part C: Methods

Volumn 17, Issue 9, 2011, Pages 895-906

  Khoo, Cheen Peen ^a,b   Micklem, Kingsley ^b   Watt, Suzanne M ^a,b  

^a NHS BLOOD AND TRANSPLANT   (United Kingdom)

^b UNIVERSITY OF OXFORD   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ASSAYS; TISSUE;

ANGIOGENESIS; BASEMENT MEMBRANE; COMPARATIVE ANALYSIS; COMPARISON OF METHODS; EASE OF USE; GROWTH FACTOR; HEXAGONAL STRUCTURES; IN-VITRO; IN-VITRO ASSAYS; PROTEASE ACTIVITIES; QUANTIFICATION METHODS; SURAMIN; TISSUE REPAIR; UMBILICAL CORD BLOOD;

ENDOTHELIAL CELLS;

ANGIOGENESIS INHIBITOR; COLLAGEN; INDOLE DERIVATIVE; LAMININ; MATRIGEL; ORANTINIB; PROTEOGLYCAN; PYRROLE DERIVATIVE; SIGNAL PEPTIDE; SURAMIN;

ANGIOGENESIS; ARTICLE; BIOASSAY; CLONOGENIC ASSAY; COMPARATIVE STUDY; COMPUTER PROGRAM; CYTOLOGY; DRUG COMBINATION; DRUG EFFECT; ENDOTHELIUM CELL; FETUS BLOOD; HUMAN; IMAGE PROCESSING; METABOLISM; METHODOLOGY; PHENOTYPE; VASCULAR ENDOTHELIUM;

ANGIOGENESIS INHIBITORS; BIOLOGICAL ASSAY; COLLAGEN; COLONY-FORMING UNITS ASSAY; DRUG COMBINATIONS; ENDOTHELIAL CELLS; ENDOTHELIUM, VASCULAR; FETAL BLOOD; HUMANS; IMAGE PROCESSING, COMPUTER-ASSISTED; INDOLES; INTERCELLULAR SIGNALING PEPTIDES AND PROTEINS; LAMININ; NEOVASCULARIZATION, PHYSIOLOGIC; PHENOTYPE; PROTEOGLYCANS; PYRROLES; SOFTWARE; SURAMIN;

EID: 80052230962     PISSN: 19373384     EISSN: 19373392     Source Type: Journal    
DOI: 10.1089/ten.tec.2011.0150     Document Type: Article

References (38)

1. Watt, S.M., Athanassopoulos, A., Harris, A.L., and Tsaknakis, G. Human endothelial stem/progenitor cells, angiogenic factors and vascular repair. J R Soc Interface 7 Suppl 6, S731, 2010.
2. Critser, P.J., and Yoder, M.C. Endothelial colony-forming cell role in neoangiogenesis and tissue repair. Curr Opin Organ Transplant 15, 68, 2010.
3. De Smet, F., Segura, I., De Bock, K., Hohensinner, P.J., and Carmeliet, P. Mechanisms of vessel branching: filopodia on endothelial tip cells lead the way. Arterioscler Thromb Vasc Biol 29, 639, 2009.
4. Crabtree, B., and Subramanian, V. Behavior of endothelial cells on Matrigel and development of a method for a rapid and reproducible in vitro angiogenesis assay. In Vitro Cell Dev Biol Anim 43, 87, 2007.
5. Zhang, Y., Fisher, N., Newey, S.E., Smythe, J., Tatton, L., Tsaknakis, G., Forde, S.P., Carpenter, L., Athanassopoulos, T., Hale, S.J., Ferguson, D.J., Tyler, M.P., and Watt, S.M. The impact of proliferative potential of umbilical cordderived endothelial progenitor cells and hypoxia on vascular tubule formation in vitro. Stem Cells Dev 18, 359, 2009.
6. Ingram, D.A., Mead, L.E., Tanaka, H., Meade, V., Fenoglio, A., Mortell, K., Pollok, K., Ferkowicz, M.J., Gilley, D., and Yoder, M.C. Identification of a novel hierarchy of endothelial progenitor cells using human peripheral and umbilical cord blood. Blood 104, 2752, 2004.
7. Arnaoutova, I., and Kleinman, H.K. In vitro angiogenesis: endothelial cell tube formation on gelled basement membrane extract. Nat Protoc 5, 628, 2010.
8. Liekens, S., De Clercq, E., and Neyts, J. Angiogenesis: regulators and clinical applications. Biochem Pharmacol 61, 253, 2001.
9. Lin, Y., Weisdorf, D.J., Solovey, A., and Hebbel, R.P. Origins of circulating endothelial cells and endothelial outgrowth from blood. J Clin Invest 105, 71, 2000.
10. Merks, R.M., Brodsky, S.V., Goligorksy, M.S., Newman, S.A., and Glazier, J.A. Cell elongation is key to in silico replication of in vitro vasculogenesis and subsequent remodeling. Dev Biol 289, 44, 2006.
11. Arnaoutova, I., George, J., Kleinman, H.K., and Benton, G. The endothelial cell tube formation assay on basement membrane turns 20: state of the science and the art. Angiogenesis 12, 267, 2009.
12. Donovan, D., Brown, N.J., Bishop, E.T., and Lewis, C.E. Comparison of three in vitro human "angiogenesis" assays with capillaries formed in vivo. Angiogenesis 4, 113, 2001.
13. Guidolin, D., Vacca, A., Nussdorfer, G.G., and Ribatti, D. A new image analysis method based on topological and fractal parameters to evaluate the angiostatic activity of docetaxel by using the Matrigel assay in vitro. Microvasc Res 67, 117, 2004.
14. Tong, E.Y., Collins, G.C., Greene-Colozzi, A.E., Chen, J.L., Manos, P.D., Judkins, K.M., Lee, J.A., Ophir, M.J., Laliberte, F.M., and Levesque, T.J. Motion-based angiogenesis analysis: a simple method to quantify blood vessel growth. Zebrafish 6, 239, 2009.
15. Laird, A.D., Vajkoczy, P., Shawver, L.K., Thurnher, A., Liang, C., Mohammadi, M., Schlessinger, J., Ullrich, A., Hubbard, S.R., Blake, R.A., Fong, T.A., Strawn, L.M., Sun, L., Tang, C., Hawtin, R., Tang, F., Shenoy, N., Hirth, K.P., McMahon, G., and Cherrington. SU6668 is a potent antiangiogenic and antitumor agent that induces regression of established tumors. Cancer Res 60, 4152, 2000.
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17. Kumar, R., Yoneda, J., Bucana, C.D., and Fidler, I.J. Regulation of distinct steps of angiogenesis by different angiogenic molecules. Int J Oncol 12, 749, 1998.
18. Traktuev, D.O., Prater, D.N., Merfeld-Clauss, S., Sanjeevaiah, A.R., Saadatzadeh, M.R., Murphy, M., Johnstone, B.H., Ingram, D.A., and March, K.L. Robust functional vascular network formation in vivo by cooperation of adipose progenitor and endothelial cells. Circ Res 104, 1410, 2009.
19. Adighibe, O., Micklem, K., Campo, L., Ferguson, M., Harris, A., Pozos, R., Gatter, K., and Pezzella, F. Is nonangiogenesis a novel pathway for cancer progression? A study using 3-dimensional tumour reconstructions. Br J Cancer 94, 1176, 2006.
20. Watt, S.M., Athanassopoulos, A., Harris, A.L., and Tsaknakis, G. Human endothelial stem/progenitor cells, angiogenic factors and vascular repair. J R Soc Interface 7 Suppl 6, S731, 2010.
21. Critser, P.J., and Yoder, M.C. Endothelial colony-forming cell role in neoangiogenesis and tissue repair. Curr Opin Organ Transplant 15, 68, 2010.
22. De Smet, F., Segura, I., De Bock, K., Hohensinner, P.J., and Carmeliet, P. Mechanisms of vessel branching: filopodia on endothelial tip cells lead the way. Arterioscler Thromb Vasc Biol 29, 639, 2009.
23. Crabtree, B., and Subramanian, V. Behavior of endothelial cells on Matrigel and development of a method for a rapid and reproducible in vitro angiogenesis assay. In Vitro Cell Dev Biol Anim 43, 87, 2007.
24. Zhang, Y., Fisher, N., Newey, S.E., Smythe, J., Tatton, L., Tsaknakis, G., Forde, S.P., Carpenter, L., Athanassopoulos, T., Hale, S.J., Ferguson, D.J., Tyler, M.P., and Watt, S.M. The impact of proliferative potential of umbilical cordderived endothelial progenitor cells and hypoxia on vascular tubule formation in vitro. Stem Cells Dev 18, 359, 2009.
25. Ingram, D.A., Mead, L.E., Tanaka, H., Meade, V., Fenoglio, A., Mortell, K., Pollok, K., Ferkowicz, M.J., Gilley, D., and Yoder, M.C. Identification of a novel hierarchy of endothelial progenitor cells using human peripheral and umbilical cord blood. Blood 104, 2752, 2004.
26. Arnaoutova, I., and Kleinman, H.K. In vitro angiogenesis: endothelial cell tube formation on gelled basement membrane extract. Nat Protoc 5, 628, 2010.
27. Liekens, S., De Clercq, E., and Neyts, J. Angiogenesis: regulators and clinical applications. Biochem Pharmacol 61, 253, 2001.
28. Lin, Y., Weisdorf, D.J., Solovey, A., and Hebbel, R.P. Origins of circulating endothelial cells and endothelial outgrowth from blood. J Clin Invest 105, 71, 2000.
29. Merks, R.M., Brodsky, S.V., Goligorksy, M.S., Newman, S.A., and Glazier, J.A. Cell elongation is key to in silico replication of in vitro vasculogenesis and subsequent remodeling. Dev Biol 289, 44, 2006.
30. Arnaoutova, I., George, J., Kleinman, H.K., and Benton, G. The endothelial cell tube formation assay on basement membrane turns 20: state of the science and the art. Angiogenesis 12, 267, 2009.
31. Donovan, D., Brown, N.J., Bishop, E.T., and Lewis, C.E. Comparison of three in vitro human "angiogenesis" assays with capillaries formed in vivo. Angiogenesis 4, 113, 2001.
32. Guidolin, D., Vacca, A., Nussdorfer, G.G., and Ribatti, D. A new image analysis method based on topological and fractal parameters to evaluate the angiostatic activity of docetaxel by using the Matrigel assay in vitro. Microvasc Res 67, 117, 2004.
33. Tong, E.Y., Collins, G.C., Greene-Colozzi, A.E., Chen, J.L., Manos, P.D., Judkins, K.M., Lee, J.A., Ophir, M.J., Laliberte, F.M., and Levesque, T.J. Motion-based angiogenesis analysis: a simple method to quantify blood vessel growth. Zebrafish 6, 239, 2009.
34. Laird, A.D., Vajkoczy, P., Shawver, L.K., Thurnher, A., Liang, C., Mohammadi, M., Schlessinger, J., Ullrich, A., Hubbard, S.R., Blake, R.A., Fong, T.A., Strawn, L.M., Sun, L., Tang, C., Hawtin, R., Tang, F., Shenoy, N., Hirth, K.P., McMahon, G., and Cherrington. SU6668 is a potent antiangiogenic and antitumor agent that induces regression of established tumors. Cancer Res 60, 4152, 2000.
35. Staton, C.A., Reed, M.W., and Brown, N.J. A critical analysis of current in vitro and in vivo angiogenesis assays. Int J Exp Pathol 90, 195, 2009.
36. Kumar, R., Yoneda, J., Bucana, C.D., and Fidler, I.J. Regulation of distinct steps of angiogenesis by different angiogenic molecules. Int J Oncol 12, 749, 1998.
37. Traktuev, D.O., Prater, D.N., Merfeld-Clauss, S., Sanjeevaiah, A.R., Saadatzadeh, M.R., Murphy, M., Johnstone, B.H., Ingram, D.A., and March, K.L. Robust functional vascular network formation in vivo by cooperation of adipose progenitor and endothelial cells. Circ Res 104, 1410, 2009.
38. Adighibe, O., Micklem, K., Campo, L., Ferguson, M., Harris, A., Pozos, R., Gatter, K., and Pezzella, F. Is nonangiogenesis a novel pathway for cancer progression? A study using 3-dimensional tumour reconstructions. Br J Cancer 94, 1176, 2006.