Methionine sulfoximine resistance in mycobacterium tuberculosis is due to a single nucleotide deletion resulting in increased expression of the major glutamine synthetase, GlnA1

Microbial Drug Resistance

Volumn 17, Issue 3, 2011, Pages 351-355

  Carroll, Paul ^a   Waddell, Simon J ^b   Butcher, Philip D ^b   Parish, Tanya ^a,c  

^a QUEEN MARY UNIVERSITY OF LONDON   (United Kingdom)

^b ST GEORGE'S UNIVERSITY OF LONDON   (United Kingdom)

^c INFECTIOUS DISEASE RESEARCH INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GLNA1 ENZYME; GLUTAMATE AMMONIA LIGASE; METHIONINE SULFOXIMINE; UNCLASSIFIED DRUG;

ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL GROWTH; BACTERIAL STRAIN; BACTERIAL SURVIVAL; CONTROLLED STUDY; DRUG EFFECT; GENE DELETION; MYCOBACTERIUM TUBERCULOSIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION;

ANTI-BACTERIAL AGENTS; BACTERIAL PROTEINS; DRUG RESISTANCE, BACTERIAL; GENE REGULATORY NETWORKS; GLUTAMATE-AMMONIA LIGASE; METHIONINE SULFOXIMINE; MYCOBACTERIUM TUBERCULOSIS; SEQUENCE DELETION; UP-REGULATION;

MYCOBACTERIUM TUBERCULOSIS;

EID: 80052204197     PISSN: 10766294     EISSN: 19318448     Source Type: Journal    
DOI: 10.1089/mdr.2010.0125     Document Type: Article

References (13)

1. Carroll, P., C.A. Pashley, and T. Parish. 2008. Functional analysis of GlnE, an essential adenylyl transferase in Mycobacterium tuberculosis. J. Bacteriol. 190:4894-4902. (Pubitemid 351991057)
2. Cole, S.T., R. Brosch, J. Parkhill, T. Garnier, C. Churcher, D. Harris, S.V. Gordon, K. Eiglmeier, S. Gas, C.E. Barry, F. Tekaia, K. Badcock, D. Basham, D. Brown, T. Chillingworth, R. Connor, R. Davies, K. Devlin, T. Feltwell, S. Gentles, N. Hamlin, S. Holroyd, T. Hornby, K. Jagels, A. Krogh, J. McLean, S. Moule, L. Murphy, K. Oliver, J. Osborne, M.A. Quail, M.A. Rajandream, J. Rogers, S. Rutter, K. Seeger, J. Skelton, R. Squares, S. Squares, J.E. Sulston, K. Taylor, S. Whitehead, and B.G. Barrell. 1998. Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence. Nature 393:537-544. (Pubitemid 28319235)
3. Darrow, R.A., and R.R. Knotts. 1977. Two forms of glutamine synthetase in free-living root-nodule bacteria. Biochem. Biophys. Res. Commun. 78:554-559. (Pubitemid 8214717)
4. Harth, G., and M.A. Horwitz. 1997. Expression and efficient export of enzymatically active Mycobacterium tuberculosis glutamine synthetase in Mycobacterium smegmatis and evidence that the information for export is contained within the protein. J. Biol. Chem. 272:22728-22735. (Pubitemid 27386090)
5. Harth, G., and M.A. Horwitz. 1999 An inhibitor of exported Mycobacterium tuberculosis glutamine synthetase selectively blocks the growth of pathogenic mycobacteria in axenic culture and in human monocytes: Extracellular proteins as potential novel drug targets. J. Exp. Med. 189:1425-1435. (Pubitemid 29227528)
6. Harth, G., S. Maslesa-Galic, M.V. Tullius, and M.A. Horwitz. 2005. All four Mycobacterium tuberculosis glnA genes encode glutamine synthetase activities but only GlnA1 is abundantly expressed and essential for bacterial homeostasis. Mol. Microbiol. 58:1157-1172. (Pubitemid 41667232)
7. Lee, S., B.Y. Jeon, S. Bardarov, M. Chen, S.L. Morris, and W.R. Jacobs. 2006. Protection elicited by two glutamine auxotrophs of Mycobacterium tuberculosis and in vivo growth phenotypes of the four unique glutamine synthetase mutants in a murine model. Infect. Immun. 74:6491-6495. (Pubitemid 44657432)
8. Parish, T., and N.G. Stoker. 2000. glnE is an essential gene in Mycobacterium tuberculosis. J. Bacteriol. 182:5715-5720.
9. Ronzio, R.A., and A. Meister. 1968. Phosphorylation of methionine sulfoximine by glutamine synthetase. Proc. Natl. Acad. Sci. U.S.A. 59:164-170.
10. Stewart, G.R., L. Wernisch, R. Stabler, J.A. Mangan, J. Hinds, K.G. Laing, P.D. Butcher, and D.B. Young. 2002. The heat shock response of Mycobacterium tuberculosis: Linking gene expression, immunology and pathogenesis. Comp. Funct. Genomics 3:348-351. (Pubitemid 34960000)
11. Stewart, G.R., L. Wernisch, R. Stabler, J.A. Mangan, J. Hinds, K.G. Laing, D.B. Young, and P.D. Butcher. 2002. Dissection of the heat-shock response in Mycobacterium tuberculosis using mutants and microarrays. Microbiology 148:3129-3138. (Pubitemid 35243712)
12. Waddell, S.J., and P.D. Butcher. 2010. Use of DNA arrays to study transcriptional responses to anitmycobacterial compounds. Methods Mol. Biol. 642:75-91.
13. Waddell, S.J., R.A. Stabler, K.G. Laing, K. L., R.C. Reynolds, and G.S. Besra. 2004. The use of microarray analysis to determine the gene expression profiles of Mycobacterium tuberculosis in response to anti-bacterial compounds. Tubercule 84:263-274. (Pubitemid 38857859)