Diagnosis of vaginal discharge by wet mount microscopy: A simple and underrated method

Obstetrical and Gynecological Survey

Volumn 66, Issue 6, 2011, Pages 359-368

  Mylonas, Ioannis ^a   Bergauer, Florian ^a  

^a UNIVERSITY OF MUNICH   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

AMOXICILLIN; AZITHROMYCIN; CEPHALOSPORIN; CLINDAMYCIN; CLOTRIMAZOLE; ERYTHROMYCIN; FLUCONAZOLE; METRONIDAZOLE; NITROIMIDAZOLE DERIVATIVE; NYSTATIN; QUINOLINE DERIVED ANTIINFECTIVE AGENT; TETRACYCLINE; TINIDAZOLE;

ATROPHIC VAGINITIS; CANDIDA ALBICANS; CHLAMYDIASIS; DIAGNOSTIC ACCURACY; DIAGNOSTIC PROCEDURE; GARDNERELLA INFECTION; GARDNERELLA VAGINALIS; GONORRHEA; GROUP A STREPTOCOCCAL INFECTION; GROUP B STREPTOCOCCAL INFECTION; HUMAN; HYDROPS; LACTOBACILLUS; LOW BIRTH WEIGHT; MENSTRUAL CYCLE; MICROSCOPY; PATIENT COMPLIANCE; PHASE CONTRAST MICROSCOPY; PHYSIOLOGICAL PROCESS; PREGNANCY; PREMATURITY; REVIEW; SENSITIVITY AND SPECIFICITY; SEXUALLY TRANSMITTED DISEASE; SINGLE DRUG DOSE; STAPHYLOCOCCUS INFECTION; TRICHOMONAS VAGINALIS; TRICHOMONIASIS; UTERINE CERVICITIS; UTERUS CARCINOMA; UTERUS SARCOMA; VAGINA ATROPHY; VAGINA CANDIDIASIS; VAGINA DISCHARGE; VAGINITIS; WET MOUNT MICROSCOPY;

CANDIDIASIS, VULVOVAGINAL; FEMALE; GENITAL NEOPLASMS, FEMALE; HUMANS; MICROSCOPY; TRICHOMONAS VAGINITIS; UTERINE CERVICITIS; VAGINAL DISCHARGE; VAGINAL SMEARS; VAGINOSIS, BACTERIAL;

EID: 80052170392     PISSN: 00297828     EISSN: 15339866     Source Type: Journal    
DOI: 10.1097/OGX.0b013e31822bdf31     Document Type: Review

References (68)

1. Mylonas I, Friese K. Genital discharge in women. MMW Fortschr Med. 2007;149:42-46; quiz 47. (Pubitemid 47528639)
2. Mylonas I, Friese K. Infektionen in der Gynä kologie und Geburtshilfe. Mü nchen: Elsevier Verlag-Urban & Fischer Verlag; 2009.
3. Donders GG, Vereecken A, Dekeersmaecker A, et al. Wet mount microscopy reflects functional vaginal lactobacillary flora better than Gram stain. J Clin Pathol. 2000;53:308-313.
4. Donders GG, Bosmans E, Dekeersmaecker A, et al. Pathogenesis of abnormal vaginal bacterial flora. Am J Obstet Gynecol. 2000;182:872-878. (Pubitemid 30228459)
5. Landers DV, Wiesenfeld HC, Heine RP, et al. Predictive value of the clinical diagnosis of lower genital tract infection in women. Am J Obstet Gynecol. 2004;190:1004-1010. (Pubitemid 38561515)
6. Saling E. Effective measures for the prevention of late abortions and premature births. Early Hum Dev. 1992;29:15-20.
7. Hoyme UB, Saling E. Efficient prematurity prevention is possible by pH-self measurement and immediate therapy of threatening ascending infection. Eur J Obstet Gynecol Reprod Biol. 2004;115:148-153. (Pubitemid 38953030)
8. Dö derlein A. Das Scheidensekret und seine Bedeutung fü r das Puerperalfieber. Leipzig: Verlag van Eduard Besold; 1892.
9. Weissenbacher ER, Spitzbart H, Soljanik I. Recurrent infections of female genitalia. Weak immunity or wrong therapy? MMW Fortschr Med. 2003;145:40-42.
10. Donders GG. Microscopy of the bacterial flora on fresh vaginal smears. Infect Dis Obstet Gynecol. 1999;7:177-179.
11. Owen MK, Clenney TL. Management of vaginitis. Am Fam Physician. 2004;70:2125-2132.
12. Foxman B, Marsh JV, Gillespie B, et al. Frequency and response to vaginal symptoms among white and African American women: results of a random digit dialing survey. J Womens Health. 1998;7:1167-1174.
13. Colli E, Bertulessi C, Landoni M, et al. Bacterial vaginosis in pregnancy and preterm birth: evidence from the literature. J Int Med Res. 1996;24:317-324. (Pubitemid 26261730)
14. Simoes JA, Giraldo PC, Faundes A. Prevalence of cervicovaginal infections during gestation and accuracy of clinical diagnosis. Infect Dis Obstet Gynecol. 1998;6:129-133. (Pubitemid 28453238)
15. Shanon H. Diagnostic microbiology of bacterial vaginosis. Am J Obstet Gynecol. 1993;165:1240-1244.
16. Allsworth JE, Peipert JF. Prevalence of bacterial vaginosis: 2001-2004 National Health and Nutrition Examination Survey data. Obstet Gynecol. 2007;109:114-120. (Pubitemid 46035517)
17. Srinivasan U, Misra D, Marazita ML, et al. Vaginal and oral microbes, host genotype and preterm birth. Med Hypotheses. 2009;73:963-975.
18. Hillier SL. Diagnostic microbiology of bacterial vaginosis. Am J Obstet Gynecol. 1993;169:455-459. (Pubitemid 23259055)
19. Gravett MG, Hitti J, Hess DL, et al. Intrauterine infection and preterm delivery: evidence for activation of the fetal hypothalamic-pituitary-adrenal axis. Am J Obstet Gynecol. 2000;182:1404-1413. (Pubitemid 30437407)
20. Goldenberg RL, Hauth JC, Andrews WW. Intrauterine infection and preterm delivery. N Engl J Med. 2000;342:1500-1507. (Pubitemid 30318275)
21. Guaschino S, De Seta F, Piccoli M, et al. Aetiology of preterm labour: bacterial vaginosis. BJOG. 2006;113(suppl 3):46-51. (Pubitemid 44934666)
22. Koumans EH, Markowitz LE, Hogan V. Indications for therapy and treatment recommendations for bacterial vaginosis in nonpregnant and pregnant women: a synthesis of data. Clin Infect Dis. 2002;35:S152-S172. (Pubitemid 35178327)
23. Hogben M, St Lawrence JS, Kasprzyk D, et al. Sexually transmitted disease screening by United States obstetricians and gynecologists. Obstet Gynecol. 2002;100:801-807. (Pubitemid 35300041)
24. Myer L, Denny L, Telerant R, et al. Bacterial vaginosis and susceptibility to HIV infection in South African women: a nested case-control study. J Infect Dis. 2005;192:1372-1380. (Pubitemid 41401150)
25. Cu-Uvin S, Hogan JW, Caliendo AM, et al. Association between bacterial vaginosis and expression of human immunodeficiency virus type 1 RNA in the female genital tract. Clin Infect Dis. 2001;33:894-896. (Pubitemid 32831848)
26. Cohn JA, Hashemi FB, Camarca M, et al. HIV-inducing factor in cervicovaginal secretions is associated with bacterial vaginosis in HIV-1-infected women. J Acquir Immune Defic Syndr. 2005;39:340-346. (Pubitemid 40923216)
27. Martin HL, Richardson BA, Nyange PM, et al. Vaginal lactobacilli, microbial flora, and risk of human immunodeficiency virus type 1 and sexually transmitted disease acquisition. J Infect Dis. 1999;180:1863-1868.
28. Cherpes TL, Meyn LA, Krohn MA, et al. Association between acquisition of herpes simplex virus type 2 in women and bacterial vaginosis. Clin Infect Dis. 2003;37:319-325. (Pubitemid 36976487)
29. Verstraelen H, Verhelst R. Bacterial vaginosis: an update on diagnosis and treatment. Expert Rev Anti Infect Ther. 2009;7: 1109-1124.
30. Amsel R, Totten PA, Spiegel CA, et al. Nonspecific vaginitis. Diagnostic criteria and microbial and epidemiologic associations. Am J Med. 1983;74:14-22. (Pubitemid 13221599)
31. Schwebke JR, Desmond RA, Oh MK. Predictors of bacterial vaginosis in adolescent women who douche. Sex Transm Dis. 2004;31:433-436. (Pubitemid 38812928)
32. Hillier SL, Krohn MA, Rabe LK, et al. The normal vaginal flora, H2O2-producing lactobacilli, and bacterial vaginosis in pregnant women. Clin Infect Dis. 1993;16(suppl 4):S273-S281. (Pubitemid 23157927)
33. Workowski KA, Berman S; Centers for Disease Control and Prevention. Sexually transmitted diseases treatment guidelines. MMWR Recomm Rep. 2010;59:1-110.
34. McDonald H, Brocklehurst P, Parsons J. Antibiotics for treating bacterial vaginosis in pregnancy. Cochrane Database Syst Rev. 2005:CD000262.
35. Oduyebo OO, Anorlu RI, Ogunsola FT. The effects of antimicrobial therapy on bacterial vaginosis in non-pregnant women. Cochrane Database Syst Rev. 2009:CD006055.
36. Beigi RH, Austin MN, Meyn LA, et al. Antimicrobial resistance associated with the treatment of bacterial vaginosis. Am J Obstet Gynecol. 2004;191:1124-1129. (Pubitemid 39421560)
37. Schwebke JR, Desmond RA. A randomized trial of the duration of therapy with metronidazole plus or minus azithromycin for treatment of symptomatic bacterial vaginosis. Clin Infect Dis. 2007;44:213-219. (Pubitemid 46079983)
38. Sobel JD. Vulvovaginal candidosis. Lancet. 2007;369:1961-1971. (Pubitemid 46874580)
39. Sobel JD, Faro S, Force RW, et al. Vulvovaginal candidiasis: epidemiologic, diagnostic, and therapeutic considerations. Am J Obstet Gynecol. 1998;178:203-211. (Pubitemid 28124116)
40. Mendling W, Seebacher C. Guideline vulvovaginal candidosis: guideline of the German Dermatological Society, the German Speaking Mycological Society and the Working Group for Infections and Infectimmunology of the German Society for Gynecology and Obstetrics. Mycoses. 2003;46:365-369. (Pubitemid 37412034)
41. Sobel JD. What's new in bacterial vaginosis and trichomoniasis? Infect Dis Clin North Am. 2005;19:387-406. (Pubitemid 40814421)
42. Huppert JS. Trichomoniasis in teens: an update. Curr Opin Obstet Gynecol. 2009;21:371-378.
43. Klebanoff MA, Carey JC, Hauth JC, et al. Failure of metronidazole to prevent preterm delivery among pregnant women with asymptomatic Trichomonas vaginalis infection. N Engl J Med. 2001;345:487-493. (Pubitemid 32758500)
44. Cotch MF, Pastorek JG II, Nugent RP, et al. Trichomonas vaginalis associated with low birth weight and preterm delivery. The Vaginal Infections and Prematurity Study Group. Sex Transm Dis. 1997;24:353-360. (Pubitemid 27306585)
45. Riduan JM, Hillier SL, Utomo B, et al. Bacterial vaginosis and prematurity in Indonesia: association in early and late pregnancy. Am J Obstet Gynecol. 1993;169:175-178. (Pubitemid 23223954)
46. Meis PJ, Goldenberg RL, Mercer B, et al. The preterm prediction study: significance of vaginal infections. National Institute of Child Health and Human Development Maternal-Fetal Medicine Units Network. Am J Obstet Gynecol. 1995;173:1231-1235.
47. Hardy PH, Hardy JB, Nell EE, et al. Prevalence of six sexually transmitted disease agents among pregnant inner-city adolescents and pregnancy outcome. Lancet. 1984;2:333-337.
48. Riggs MA, Klebanoff MA. Treatment of vaginal infections to prevent preterm birth: a meta-analysis. Clin Obstet Gynecol. 2004;47:796-807; discussion 881-792. (Pubitemid 39463472)
49. McGregor JA, French JI, Richter R, et al. Cervicovaginal microflora and pregnancy outcome: results of a double-blind, placebo-controlled trial of erythromycin treatment. Am J Obstet Gynecol. 1990;163:1580-1591.
50. McGregor JA, French JI, Parker R, et al. Prevention of premature birth by screening and treatment for common genital tract infections: results of a prospective controlled evaluation. Am J Obstet Gynecol. 1995;173:157-167.
51. Gulmezoglu AM. Interventions for trichomoniasis in pregnancy. Cochrane Database Syst Rev. 2002:CD000220.
52. Kigozi GG, Brahmbhatt H, Wabwire-Mangen F, et al. Treatment of Trichomonas in pregnancy and adverse outcomes of pregnancy: a subanalysis of a randomized trial in Rakai, Uganda. Am J Obstet Gynecol. 2003;189:1398-1400. (Pubitemid 37542180)
53. Brown HL, Fuller DD, Jasper LT, et al. Clinical evaluation of affirm VPIII in the detection and identification of Trichomonas vaginalis, Gardnerella vaginalis, and Candida species in vaginitis/vaginosis. Infect Dis Obstet Gynecol. 2004;12:17-21. (Pubitemid 38925343)
54. Wiese W, Patel SR, Patel SC, et al. A meta-analysis of the Papanicolaou smear and wet mount for the diagnosis of vaginal trichomoniasis. Am J Med. 2000;108:301-308. (Pubitemid 30110459)
55. Krieger JN, Tam MR, Stevens CE, et al. Diagnosis of trichomoniasis. Comparison of conventional wet-mount examination with cytologic studies, cultures, and monoclonal antibody staining of direct specimens. JAMA. 1988;259:1223-1227. (Pubitemid 18058836)
56. Nye MB, Schwebke JR, Body BA. Comparison of APTIMA Trichomonas vaginalis transcription-mediated amplification to wet mount microscopy, culture, and polymerase chain reaction for diagnosis of trichomoniasis in men and women. Am J Obstet Gynecol. 2009;200:188.e181-e187.
57. Carney JA, Unadkat P, Yule A, et al. New rapid latex agglutination test for diagnosing Trichomonas vaginalis infection. J Clin Pathol. 1988;41:806-808.
58. Adu-Sarkodie Y, Opoku BK, Danso KA, et al. Comparison of latex agglutination, wet preparation, and culture for the detection of Trichomonas vaginalis. Sex Transm Infect. 2004;80: 201-203. (Pubitemid 38746195)
59. Mayta H, Gilman RH, Calderon MM, et al. 18S ribosomal DNA-based PCR for diagnosis of Trichomonas vaginalis. J Clin Microbiol. 2000;38:2683-2687. (Pubitemid 30485150)
60. Van Der Pol B, Kraft CS, Williams JA. Use of an adaptation of a commercially available PCR assay aimed at diagnosis of chlamydia and gonorrhea to detect Trichomonas vaginalis in urogenital specimens. J Clin Microbiol. 2006;44:366-373. (Pubitemid 43222224)
61. Huppert JS, Mortensen JE, Reed JL, et al. Rapid antigen testing compares favorably with transcription-mediated amplification assay for the detection of Trichomonas vaginalis in young women. Clin Infect Dis. 2007;45:194-198. (Pubitemid 47041010)
62. Schwebke JR, Venglarik MF, Morgan SC. Delayed versus immediate bedside inoculation of culture media for diagnosis of vaginal trichomonosis. J Clin Microbiol. 1999;37:2369-2370. (Pubitemid 29277813)
63. Swygard H, Miller WC, Kaydos-Daniels SC, et al. Targeted screening for Trichomonas vaginalis with culture using a twostep method in women presenting for STD evaluation. Sex Transm Dis. 2004;31:659-664. (Pubitemid 39426397)
64. Huppert JS, Biro F, Lan D, et al. Urinary symptoms in adolescent females: STI or UTI? J Adolesc Health. 2007;40:418-424. (Pubitemid 46590023)
65. Nanda N, Michel RG, Kurdgelashvili G, et al. Trichomoniasis and its treatment. Expert Rev Anti Infect Ther. 2006;4:125-135.
66. Forna F, Gulmezoglu AM. Interventions for treating trichomoniasis in women. Cochrane Database Syst Rev. 2003:CD000218.
67. Stampler KM, Lieberman A, Fraga M, et al. Vaginal wet mounts on asymptomatic adolescent females; are they beneficial? J Pediatr Adolesc Gynecol. 2008;21:227-230. (Pubitemid 352004266)
68. Datta SD, Sternberg M, Johnson RE, et al. Gonorrhea and chlamydia in the United States among persons 14 to 39 years of age, 1999 to 2002. Ann Intern Med. 2007;147:89-96. (Pubitemid 351664612)