Impact of collection method on assessment of semen HIV RNA viral load

PLoS ONE

Volumn 6, Issue 8, 2011, Pages

  Osborne, Brendan J W ^a   Sheth, Prameet M ^a   Kovacs, Colin ^c   Mazzulli, Tony ^a,d   Kaul, Rupert ^a,b  

^a UNIVERSITY OF TORONTO   (Canada)

^b UNIVERSITY OF TORONTO   (Canada)

^c Maple Leaf Medical Centre   (Canada)

^d MOUNT SINAI HOSPITAL   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

VIRUS RNA;

ARTICLE; BLOOD SAMPLING; CONTROLLED STUDY; DILUTION; HUMAN; HUMAN IMMUNODEFICIENCY VIRUS 1; HUMAN IMMUNODEFICIENCY VIRUS INFECTED PATIENT; INTERMETHOD COMPARISON; MALE; MALE HOMOSEXUAL; MASTURBATION; NONHUMAN; RNA ANALYSIS; SAMPLING; SEMEN ANALYSIS; SEMINAL PLASMA; TRANSPORT MEDIUM; VIRUS LOAD; BLOOD; GENETICS; LABORATORY DIAGNOSIS; METHODOLOGY; SPERM; VIROLOGY;

HIV-1; HUMANS; MALE; RNA, VIRAL; SEMEN; SPECIMEN HANDLING; VIRAL LOAD;

EID: 80051838847     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0023654     Document Type: Article

References (12)

1. Global report: UNAIDS report on the global AIDS epidemic (2010) Joint United Nations Programme on HIV/AIDS (UNAIDS).
2. Quinn TC, Wawer MJ, Sewankambo N, Serwadda D, Li C, et al. (2000) Viral load and heterosexual transmission of human immunodeficiency virus type 1. Rakai Project Study Group. N Engl J Med 342: 921-929.
3. Kaul R, Pettengell C, Sheth PM, Sunderji S, Biringer A, et al. (2008) The genital tract immune milieu: an important determinant of HIV susceptibility and secondary transmission. J Reprod Immunol 77: 32-40.
4. Baeten JM, Kahle E, Lingappa JR, Coombs RW, Delany-Moretlwe S, et al. (2011) Genital HIV-1 RNA predicts risk of heterosexual HIV-1 transmission. Sci Transl Med 3: 77ra29.
5. Donnell D, Baeten JM, Kiarie J, Thomas KK, Stevens W, et al. (2010) Heterosexual HIV-1 transmission after initiation of antiretroviral therapy: a prospective cohort analysis. Lancet 375: 2092-2098.
6. Sheth PM, Kovacs C, Kemal KS, Jones RB, Raboud JM, et al. (2009) Persistent HIV RNA shedding in semen despite effective antiretroviral therapy. AIDS 23: 2050-2054.
7. Dunne AL, Mitchell F, Allen KM, Baker HW, Garland S, et al. (2003) Analysis of HIV-1 viral load in seminal plasma samples. J Clin Virol 26: 239-245.
8. Chan DJ, McNally L, Batterham M, Smith DE, (2008) Relationship between HIV-RNA load in blood and semen in antiretroviral-naive and experienced men and effect of asymptomatic sexually transmissible infections. Curr HIV Res 6: 138-142.
9. Dyer JR, Gilliam BL, Eron JJ Jr, Grosso L, Cohen MS, et al. (1996) Quantitation of human immunodeficiency virus type 1 RNA in cell free seminal plasma: comparison of NASBA with Amplicor reverse transcription-PCR amplification and correlation with quantitative culture. J Virol Methods 60: 161-170.
10. Politch JA, Tucker L, Bowman FP, Anderson DJ, (2007) Concentrations and significance of cytokines and other immunologic factors in semen of healthy fertile men. Hum Reprod 22: 2928-2935.
11. Sheth PM, Danesh A, Shahabi K, Rebbapragada A, Kovacs C, et al. (2005) HIV-specific CD8+ lymphocytes in semen are not associated with reduced HIV shedding. J Immunol 175: 4789-4796.
12. Resnick L, Veren K, Salahuddin SZ, Tondreau S, Markham PD, (1986) Stability and inactivation of HTLV-III/LAV under clinical and laboratory environments. JAMA 255: 1887-1891.