Preparation of carriers based on magnetic nanoparticles grafted polymer and immobilization for lipase

Biochemical Engineering Journal

Volumn 56, Issue 3, 2011, Pages 142-149

  Liu, Xiao ^a   Lei, Lin ^a   Li, Yanfeng ^a   Zhu, Hao ^a   Cui, Yanjun ^a   Hu, Huaiyuan ^a  

^a LANZHOU UNIVERSITY   (China)

Author keywords

Covalent binding; Electrostatic adsorption; Enzyme technology; Immobilized CRL; Reusability; Super paramagnetism

Indexed keywords

ACTIVATION REACTION; ACTIVITY RECOVERY; AMMONIUM CHLORIDE; CANDIDA RUGOSA LIPASE; COVALENT BINDING; ELECTROSTATIC ADSORPTION; ENZYME TECHNOLOGY; EPOXY CHLOROPROPANE; EPOXY GROUP; GRAFTED POLYMERS; IMMOBILIZED CRL; IMMOBILIZED LIPASE; LOADING AMOUNT; MAGNETIC MICROSPHERES; MAGNETIC NANOPARTICLES; PARAMAGNETIC PROPERTIES; POSITIVE CHARGES; SURFACE-INITIATED; VIBRATING SAMPLE MAGNETOMETRY;

ADSORPTION; AMMONIUM COMPOUNDS; CHEMICAL ACTIVATION; CHLORINE COMPOUNDS; ELECTROSTATICS; FOURIER TRANSFORM INFRARED SPECTROSCOPY; MICROSPHERES; NANOPARTICLES; PARAMAGNETIC MATERIALS; PARAMAGNETISM; REUSABILITY; SATURATION MAGNETIZATION; THERMOGRAVIMETRIC ANALYSIS; TRANSMISSION ELECTRON MICROSCOPY;

NANOMAGNETICS;

MAGNETIC NANOPARTICLE; MICROSPHERE; NANOCARRIER; POLYMACON; POLYMER; PROPANE; TRIACYLGLYCEROL LIPASE;

ADSORPTION; ANALYTIC METHOD; ARTICLE; CANDIDA RUGOSA; COVALENT BOND; ENZYME IMMOBILIZATION; INFRARED SPECTROSCOPY; MAGNETISM; PARTICLE SIZE; POLYMERIZATION; PRIORITY JOURNAL; REACTION ANALYSIS; SYNTHESIS; THERMOGRAVIMETRY; TRANSMISSION ELECTRON MICROSCOPY; VIBRATING SAMPLE MAGNETOMETRY;

CANDIDA RUGOSA;

EID: 80051801509     PISSN: 1369703X     EISSN: 1873295X     Source Type: Journal    
DOI: 10.1016/j.bej.2011.05.013     Document Type: Article

References (36)

1. Hong J., Xu D.M., Gong P.J., Sun H.W., Dong L., Yao S. Covalent binding of α-chymotrypsin on the magnetic nanogels covered by amino groups. J. Mol. Catal. B-Enzym. 2007, 45:84-90.
2. Mari P.D., Sinisterra J.V., Tsai S.W., Alcantara A.R. Carica papaya lipase (CPL): an emerging and versatile biocatalyst. Biotechnol. Adv. 2006, 24:493-499.
3. Omprakash Y., Toyoko I. Covalent-bonded immobilization of lipase on poly(phenylene sulfide) dendrimers and their hydrolysis ability. Biomacromolecules 2005, 6:2809-2814.
4. Jaeger K., Reetz M. Microbial lipase form versatile tools for biotechnology. Trends Biotechnol. 1998, 16:396-403.
5. Gardossi L., Bianchi D., Klibanov A.M. Selective acylation of peptide catalyzed by lipases in organic solvents. J. Am. Chem. Soc. 1991, 113:6328-6329.
6. Mari{dotless}a P.D., Montero J.M.S., Sinisterra J.V., Alcantara A.R. Understanding candida rugosa lipases: an overview. Biotechnol. Adv. 2006, 24:180-196.
7. Chang S.W., Shaw J.F., Yang K.H., Chang S.F., Shieh C.J. Studies of optimum conditions for covalent immobilization of candida rugosa lipase on poly(c-glutamic acid) by RSM. Bioresour. Technol. 2008, 99:2800-2805.
8. Jiang D.S., Long S.Y., Huang J., Xiao H.Y., Zhou J.Y. Immobilization of Pycnoporus sanguineus laccase on magnetic chitosan microspheres. Biochem. Eng. J. 2005, 25:15-23.
9. Lei L., Bai Y.X., Li Y.F., Yi L.X., Yang Y., Xia C.G. Study on immobilization of lipase onto magnetic microspheres with epoxy groups. J. Magn. Magn. Mater. 2009, 321:252-258.
10. Cristian G., Lucia P., Vincenzo D.F., Paoto S. Gold nanoparticles-based protease assay. Proc. Natl. Acad. Sci. U.S.A. 2006, 103:3978-3982.
11. Hong J., Gong P.J., Yu J.H., Xu D.M., Sun H.W., Yao S. Conjugation of α-chymotrypsin on a polymeric hydrophilic nanolayer covering magnetic nanoparticles. J. Mol. Catal. B-Enzym. 2006, 42:99-105.
12. Torres R., Mateo C., Fernandez L.G., Ortiz C., Fuentes M., Palomo J.M., Guisan J.M., Fernandez L.R. A novel heterofunctional epoxy-amino sepabeads for a new enzyme immobilization protocol: immobilization-stabilization of beta-galactosidase from Aspergillus oryzae. Biotechnol. Prog. 2003, 19:1056-1060.
13. Mateo C., Torres R., Fernandez L.G., Ortiz C., Fuentes M., Hidalgo A., Lopez G.F., Abian O., Palomo J.M., Betancor L., Pessela B.C.C., Guisan J.M., Fernandez L.R. Epoxy-amino groups: a new tool for improved immobilization of proteins by the epoxy method. Biomacromolecules 2003, 4:772-777.
14. Ding Y., Hu Y., Zhang L.Y., Chen Y., Jiang X.Q. Synthesis and magnetic properties of biocompatible hybrid hollow spheres. Biomacromolecules 2006, 7:1766-1772.
15. Guo Z., Bai S., Sun Y. Preparation and characterization of immobilized lipase on magnetic hydrophobic microspheres. Enzyme Microb. Technol. 2003, 32:776-782.
16. Gulay B., Yagmur T., Arica M.Y. Immobilization of b-galactosidase onto magnetic poly(GMA-MMA) beads for hydrolysis of lactose in bed reactor. Catal. Commun. 2007, 8:1094-1101.
17. Bayramoglu G., Kiralp S., Yilmaz M., Toppare L., Ari{dotless}ca M.Y. Covalent immobilization of chloroperoxidase onto magnetic beads: Catalytic properties and stability. Biochem. Eng. J. 2008, 38:180-188.
18. Yang Y., Bai Y.X., Li Y.F., Lei L., Cui Y.J., Xia C.G. Preparation and application of polymer-grafted magnetic nanoparticles for lipase immobilization. J. Magn. Magn. Mater. 2008, 320:2350-2355.
19. Wang Y.J., Caruso F. Mesoporous silica spheres as supports for enzyme immobilization and encapsulation. Chem. Mater. 2005, 17:953-961.
20. Koutsopoulos S., Oost J., Norde W. Adsorption of an endoglucanase from the hyperthermophilic Pyrococcus furiosus on hydrophobic (polystyrene) and hydrophilic (silica) surfaces increases protein heat stability. Langmuir 2004, 20:6401-6406.
21. Pessela B.C.C., Mateo C., Carrascosa A.V., Vian A., Garcia J.L., Rivas G., Alfonso C., Guisan J.M., Lafuente R.F. One-step purification, covalent immobilization, and additional stabilization of a thermophilic poly-His-tagged beta-galactosidase from Thermus sp. strain T2 by using novel heterofunctional chelate-epoxy Sepabeads. Biomacromolecules 2003, 4:107-113.
22. Bohuslav R., Alena S., Daniel H. Functionalised magnetic microspheres with hydrophilic properties for molecular diagnostic applications. Food Res. Int. 2009, 42:493-498.
23. Massart R., Cabuil V. Effect of some parameters on the formation of colloidal magnetite in alkaline-medium-yield and particle-size control. J. Chim. Phys. Phys.-Chim. Biol. 1987, 84:967-973.
24. Xu F.J., Cai Q.J., Li Y.L., Kang E.T., Neoh K.G. Covalent immobilization of glucose oxidase on well-defined poly(glycidyl methacrylate)-Si(111) hybrids from surface-initiated atom-transfer radical polymerization. Biomacromolecules 2005, 6:1012-1020.
25. Teke A.B., Baysal S.H. Immobilization of urease using glycidyl methacrylate grafted nylon-6-membranes. Process Biochem. 2007, 42:439-443.
26. Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 1976, 72:248-254.
27. Watanabe N., Ota Y., Minoda Y., Yamada K. Isolation and identification of alkaline lipase producing microorganisms: cultural conditions and some properties of crude enzymes. Agric. Biol. Chem. 1977, 41:1353-1358.
28. Sundberg L., Porath J. Preparation of adsorbents for biospecific affinity chromatography. J. Chromatogr. 1974, 90:87-98.
29. Ma Z.Y., Guan Y.P., Liu H.Z. Superparamagnetic silica nanoparticles with immobilized metal affinity ligands for protein adsorption. J. Magn. Magn. Mater. 2006, 301:469-477.
30. 2 spheres. Process Biochem. 2006, 41:770-777.
31. Bayramoglu G., Kaya B., Ari{dotless}ca M.Y. Immobilization of candida rugosa lipase onto spacer-arm attached poly(GMA-HEMA-EGDMA) microspheres. Food Chem. 2005, 92:261-268.
32. Yang Y., Bai Y.X., Li Y.F., Lei L., Cui Y.J., Xia C.G. Characterization of candida rugosa lipase immobilized onto magnetic microspheres with hydrophilicity. Process Biochem. 2008, 43:1179-1185.
33. Xue P., Xu F., Xu L.D. Epoxy-functionalized mesostructured cellular foams as effective support for covalent immobilization of penicillin G acylase. Appl. Surf. Sci. 2008, 255:1625-1630.
34. Bai Y.X., Li Y.F., Wang M.T. Study on synthesis of a hydrophilic bead carrier containing epoxy groups and its properties for glucoamylase immobilization. Enzyme Microb. Technol. 2006, 39:540-547.
35. Chaubey A., Parshad R., Koul S. Arthrobacter sp. lipase immobilization for improvement in stability and enantio selectivity. Appl. Microbiol. Biotechnol. 2006, 73:598-606.
36. Xi F.N., Wu J.M., Jia Z.S., Lin X.F. Preparation and characterization of trypsin immobilized on silica gel supported macroporous chitosan bead. Process Biochem. 2005, 40:2833-2840.