Determination of the N-glycosylation patterns of seed proteins: Applications to determine the authenticity and substantial equivalence of genetically modified (GM) crops

Journal of Agricultural and Food Chemistry

Volumn 59, Issue 16, 2011, Pages 8779-8788

  Marsh, Justin T ^a   Tryfona, Theodora ^b   Powers, Stephen J ^a   Stephens, Elaine ^b,c   Dupree, Paul ^b   Shewry, Peter R ^a   Lovegrove, Alison ^a  

^a ROTHAMSTED RESEARCH   (United Kingdom)

^b UNIVERSITY OF CAMBRIDGE   (United Kingdom)

^c MRC LABORATORY OF MOLECULAR BIOLOGY   (United Kingdom)

Author keywords

allergenicity; genetic modification; inauthentic glycosylation; MALDI ToF MS; N glycosylation

Indexed keywords

ALLERGENICITY; GENETIC MODIFICATIONS; INAUTHENTIC GLYCOSYLATION; MALDI TOF MS; N-GLYCOSYLATION;

AMYLASES; ESTERIFICATION; GENES; GENETIC ENGINEERING; MULTIVARIANT ANALYSIS; PROTEINS;

GLYCOSYLATION;

ALPHA AMYLASE INHIBITOR, PHASEOLUS VULGARIS; ALPHA-AMYLASE INHIBITOR, PHASEOLUS VULGARIS; PHASEOLIN PROTEIN, PHASEOLUS VULGARIS; PLANT LECTIN; VEGETABLE PROTEIN;

ARTICLE; CHEMISTRY; CLASSIFICATION; GENETICS; GLYCOSYLATION; MASS SPECTROMETRY; PEA; PLANT SEED; TRANSGENIC PLANT;

GLYCOSYLATION; PEAS; PLANT LECTINS; PLANT PROTEINS; PLANTS, GENETICALLY MODIFIED; SEEDS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

PHASEOLUS ACUTIFOLIUS; PHASEOLUS LUNATUS; PHASEOLUS VULGARIS; PISUM SATIVUM;

EID: 80051778024     PISSN: 00218561     EISSN: 15205118     Source Type: Journal    
DOI: 10.1021/jf2010854     Document Type: Article

References (19)

1. Yan, B.; Zhang, W.; Ding, J.; Gao, P. Sequence pattern for the occurrence of N-glycosylation in proteins J. Protein Chem. 1999, 18, 511-521 (Pubitemid 29473491)
2. Tan, L.; Leykam, J. F.; Kieliszewski, M. J. Glycosylation motifs that direct arabinogalactan addition to arabinogalactan-proteins Plant Physiol. 2003, 132, 1362-1369 (Pubitemid 36872886)
3. Hall, T. C.; Chandrasekharan, C. B.; Li, G. Phaseolin: its past, properties, regulation and future. In Seed Proteins; Shewry, P. R.; Casey, R., Eds.; Kluwer Academic Publishers: Dordrecht, The Netherlands, 1999; pp 209-240.
4. Wilson, I. B. H.; Zeleny, R.; Kolarich, D.; Staudacher, E.; Stroop, C. J. M.; Kamerling, J. P.; Altmann, F. Analysis of Asn-linked glycans from vegetable foodstuffs: widespread occurrence of Lewis a, core α1,3-linked fucose and xylose substitutions Glycobiology 2001, 11, 261-274 (Pubitemid 32600396)
5. Sanchez-Monge, R.; Gomez, L.; Barber, D.; Lopez-Otin, C.; Armentia, A.; Salcedo, G. Wheat and barley allergens associated with baker's asthma. Glycosylated subunits of the α-amylase-inhibitor family have enhanced IgE-binding capacity Biochem. J. 1992, 281, 401-405
6. Salcedo, G.; Sanchez-Monge, R.; Garcia-Casado, G.; Armentia, A.; Gomez, L.; Barber, D. The cereal α-amylase/trypsin inhibitor family associated with bakers' asthma and food allergy. In Plant Food Allergens; Mills, E. N. C.; Shewry, P. R., Eds.; Blackwell Publishing: Oxford, U.K., 2004; pp 70-86.
7. Prescott, V. E.; Campbell, P. M.; Moore, A.; Mattes, J.; Rothenberg, M. E.; Foster, P. S.; Higgins, T. J. V.; Hogan, S. P. Transgenic expression of bean α-amylase inhibitor in peas results in altered structure and immunogenicity J. Agric. Food Chem. 2005, 53, 9023-9030 (Pubitemid 41735668)
8. Bollini, R.; Vitale, A.; Chrispeels, M. J. In vivo and in vitro processing of seed reserve protein in the endoplasmic reticulum: evidence for two glycosylation steps J. Cell Biol. 1983, 96, 999-1007 (Pubitemid 13083481)
9. Sturm, A.; Van Kuik, J. A.; Vliegenthart, J. F. G.; Chrispeels, M. J. Structure, position and biosynthesis of the high mannose and the complex oligosaccharide side chains of the bean storage protein phaseolin J. Biol. Chem. 1987b, 262, 13392-13403
10. Powers, J. R.; Whitaker, J. R. Purification and chemical properties of red kidney bean (Phaseolus vulgaris) α-amylase inhibitor J. Food Biochem. 1977, 1, 217-238
11. Ciucanu, I.; Kerek, F. A simple and rapid method for the permethylation of carbohydrates Carbohydr. Res. 1984, 131, 209-217
12. Krzanowski, W. J. Principles of Multivariate Analysis: A User's Perspective; Clarendon Press: Oxford, U.K., 1988.
13. Moreno, J.; Chrispeels, M. J. A lectin gene encodes the α-amylase inhibitor of the common bean Proc. Natl. Acad. Sci. U.S.A. 1989, 86, 7885-7889
14. Altabella, T.; Chrispeels, M. J. Tobacco plants transformed with the bean α ai gene express an inhibitor of insect α-amylase in their seeds Plant Physiol. 1990, 93, 805-810 (Pubitemid 120015557)
15. Shade, R. E.; Schroeder, H. E.; Pueyo, J. J.; Tabe, L. M.; Murdock, L. L.; Higgins, T. J. V.; Chrispeels, M. J. Transgenic pea seeds expressing the α-amylase inhibitor of the common bean are resistant to bruchid beetles Bio/Technology 1994, 12, 793-796
16. Young, N. M.; Thibault, P.; Watson, D. C.; Chrispeels, M. J. Post-translational processing of two α-amylase inhibitors and an arcelin from the common bean, Phaseolus vulgaris FEBS Lett. 1999, 446, 203-206 (Pubitemid 29127277)
17. Yamaguchi, H.; Funaoka, H.; Iwamoto, H. Structures of sugar chains of the subunits of an α-amylase inhibitor from Phaseolus vulgaris white kidney beans J. Biochem. 1992, 111, 388-395
18. Sawada, S.; Takeda, Y.; Tashiro, M. Primary structures of α- and β-subunits of α-amylase inhibitors from seeds of three cultivars of Phaseolus beans J. Protein Chem. 2002, 21, 9-17 (Pubitemid 34194651)
19. Casey, R.; Domoney, C. Pea globulins. In Seed Proteins; Shewry, P. R.; Casey, R., Eds.; Kluwer Academic Publishers: Dordrecht, The Netherlands, 1999; pp 171-208.