Improved preservation of fine structure of deep-sea microorganisms by freeze-substitution after glutaraldehyde fixation

Journal of Electron Microscopy

Volumn 60, Issue 4, 2011, Pages 283-287

  Yamaguchi, Masashi ^a   Namiki, Yuichi ^a   Okada, Hitoshi ^a,b   Uematsu, Katsuyuki ^c   Tame, Akihiro ^c   Maruyama, Tadashi ^d   Kozuka, Yoshimichi ^e  

^a CHIBA UNIVERSITY   (Japan)

^b Non Profit Organization   (Japan)

^c MARINE WORKS JAPAN LTD   (Japan)

^d JAPAN AGENCY FOR MARINE EARTH SCIENCE AND TECHNOLOGY   (Japan)

^e TOKYO MEDICAL UNIVERSITY   (Japan)

Author keywords

deep sea microorganisms; freeze substitution; ultrastructure

Indexed keywords

GLUTARALDEHYDE; SEA WATER;

ANIMAL; ARTICLE; BACTERIUM; CHEMISTRY; CYTOLOGY; ELECTRON MICROSCOPY; EVALUATION; FREEZE DRYING; METHODOLOGY; MICROBIOLOGY; POLYCHAETA; TISSUE FIXATION; ULTRASTRUCTURE;

ANIMALS; BACTERIA; FREEZE SUBSTITUTION; GLUTARAL; MICROSCOPY, ELECTRON; POLYCHAETA; SEAWATER; TISSUE FIXATION;

FREEZING; MICROSTRUCTURE;

DEEP SEA; DEEP-SEA MICROORGANISM; FINE STRUCTURES; FREEZE SUBSTITUTION; GLUTARALDEHYDES; HIGH-RESOLUTION IMAGES; LIVING CELL; RAPID FREEZING; ULTRASTRUCTURE;

MICROORGANISMS;

EID: 80051744694     PISSN: 00220744     EISSN: 14779986     Source Type: Journal    
DOI: 10.1093/jmicro/dfr032     Document Type: Article

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