Smart specimen preparation for freeze substitution and serial ultrathin sectioning of yeast cells

Journal of Electron Microscopy

Volumn 58, Issue 4, 2009, Pages 261-266

  Yamaguchi, Masashi ^a   Okada, Hitoshi ^a,b   Namiki, Yuichi ^a  

^a CHIBA UNIVERSITY   (Japan)

^b Non Profit Organization   (Japan)

Author keywords

Freeze substitution; Serial thin sectioning; Transmission electron microscopy; Yeast

Indexed keywords

EPOXY RESIN;

ARTICLE; EVALUATION; FREEZE DRYING; HISTOLOGY; METHODOLOGY; MICROTOMY; SACCHAROMYCES CEREVISIAE; TRANSMISSION ELECTRON MICROSCOPY; ULTRASTRUCTURE;

EPOXY RESINS; FREEZE SUBSTITUTION; MICROSCOPY, ELECTRON, TRANSMISSION; MICROTOMY; SACCHAROMYCES CEREVISIAE; TISSUE EMBEDDING;

CELLS; CYTOLOGY; EPOXY RESINS; HIGH RESOLUTION TRANSMISSION ELECTRON MICROSCOPY;

CELL LAYERS; CELL-BE; CELL/B.E; CELL/BE; FREEZE SUBSTITUTION; SERIAL SECTIONING; SERIAL THIN SECTIONING; SINGLE LAYER; ULTRA-THIN; YEAST CELL;

YEAST;

EID: 67649209510     PISSN: 00220744     EISSN: 14779986     Source Type: Journal    
DOI: 10.1093/jmicro/dfp013     Document Type: Article

References (18)

1. Keddie F M, and Barajas L (1969) Three-dimensional reconstruction of Pityrosporum yeast cells based on serial section electron microscopy. J. Ultrastruct. Res. 29: 260-275.
2. Hoffmann H P, and Avers C J (1973) Mitochondrion of yeast: ultra- structural evidence for one giant, branched organelle per cell. Science 181: 749-751.
3. Baba M, Baba N, Ohsumi Y, Kanaya K, and Osumi M (1989) Three-dimensional analysis of morphogenesis induced by mating pheromone alpha factor in Saccharomyces cerevisiae. J. Cell Sci. 94: 207-216.
4. Winey M, Mamay C L, O'Toole E T, Mastronarde D N, Giddings T H Jr, McDonald K L, and Mcintosh J R (1995) Three-dimensional ultrastructural analysis of the Saccharomyces cerevisiae mitotic spindle. J. Cell Biol. 129: 1 601-16 1 5.
5. Winey M, Morgan G P, Straight P D, Giddings T H , and Mas- tronarde D N (2005) Three-dimensional ultrastructure of Sac- charomyces cerevisiae meiotic spindles . Mol. Biol. Cell 16: 1178-1188
6. WineyM, Yarar D, Giddings TH Jr, and Mastronarde D N (1997) Nuclear pore complex number and distribution throughout the Saccha- romyces cerevisiae cell cycle by three-dimensional reconstruction from electron micrographs of nuclear envelopes Mol. Biol. Cell 8: 2119-2132
7. Takagi T, Ishijima A S, Ochi H, and Osumi M (2003) Ultrastructure and behavior of actin cytoskeleton during cell wall formation in the fission yeast Schizosaccharomyces pombe. J. Electron Microsc. 52: 161-1 74
8. Biswas S K, Yamaguchi M, Naoe N, Takashima T, and Takeo K (2003) Quantitative three-dimensional structural analysis of Ex- ophiala dermatitidis yeast cells by freeze-substitution and serial ultra- thin sectioning J. Electron Microsc. 52: 133-143
9. Yamaguchi M (2004) Quantitative and dynamic ultrastructure of yeast cells by electron microscopy. Recent Res. Dev. Microbiol. 8: 219-243.
10. Yamaguchi M (2006) Structome of Exophiala yeast cells determined by freeze-substitution and serial ultrathin sectioning electron microscopy. Curr. Trends Microbiol. 2: 1-12 .
11. Baba M, and Osumi M (1987) Transmission and scanning electron microscopic examination of intracellular organelles in freeze- substituted Kloeckera and Saccharomyces cerevisiae yeast cells J. Electron Microsc. Tech. 5: 249-261.
12. Yamaguchi M, Biswas S K, Kita S, Aikawa E, and Takeo K (2002) Electron microscopy of pathogenic yeasts Cryptococcus neoformans and Exophiala dermatitidis by high-pressure freezing. J. Electron Microsc. 51: 21-27.
13. Jehl B, Bauer R, Dorge A, and Rick R (1981) The use of propane/ isopentane mixtures for rapid freezing of biological specimens. J. Mi- crosc. 12 3 : 307-3 079.
14. Yamaguchi M, Biswas S K, Suzuki Y, Furukawa H, Sameshima M, and Takeo K (2002) The spindle pole body duplicates in early G1 phase in the pathogenic yeast Exophiala dermatitidis: an ultrastruc- tural study . Exp. Cell Res. 279: 71-79.
15. Rowley J C III, and Moran D T (1975) A simple procedure for mounting wrinkle-free sections on Formvar-coated slot grids Ultra- microscopy1: 151-155
16. Yamaguchi M, Biswas S K, Suzuki Y, Furukawa H, and Takeo K (2003) Three-dimensional reconstruction of a pathogenic yeast Ex- ophiala dermatitidis cell by freeze-substitution and serial sectioning electron microscopy. FEMS Microbiol. Lett. 219: 17-21.
17. Yamaguchi M, Shimizu M, Yamaguchi T, Ohkusu M, and Kawamoto S (2005) Repeated use of uranyl acetate solution for section staining in transmission electron microscopy Plant Morphol. 17: 57- 59
18. Yamaguchi M, Tanaka A, and Suzuki T (1992) A support film of plasma-polymerized naphthalene for electron microscopy: method of preparation and application. J. Electron Microsc. 41: 7-13.