Accurate determination of s-phase fraction in proliferative cells by dual fluorescence and peroxidase immunohistochemistry with 5-bromo-2'-deoxyuridine (brdu) and ki67 antibodies

Journal of Histochemistry and Cytochemistry

Volumn 59, Issue 8, 2011, Pages 791-798

  Tanaka, Rina ^a   Tainaka, Motomi ^a   Ota, Takumi ^a   Mizuguchi, Naoki ^a   Kato, Hiroyuki ^a   Urabe, Shoichi ^a   Chen, Yulin ^a   Fustin, Jean Michel ^a   Yamaguchi, Yoshiaki ^a   Doi, Masao ^a   Hamada, Shinshichi ^b,c   Okamura, Hitoshi ^a  

^a KYOTO UNIVERSITY   (Japan)

^b KYOTO PREFECTURAL UNIVERSITY OF MEDICINE   (Japan)

^c Otsu Municipal Hospital   (Japan)

Author keywords

Adrenal gland; Brdu; Fluorescence immunohistochemistry; Ki67; Peroxidase immunohistochemistry; Skin; Stomach

Indexed keywords

BROXURIDINE; MONOCLONAL ANTIBODY KI 67; PEROXIDASE;

ADRENAL CORTEX; ANIMAL CELL; ANIMAL TISSUE; ANTIGEN RETRIEVAL; ARTICLE; CELL CYCLE G0 PHASE; CELL CYCLE G1 PHASE; CELL CYCLE G2 PHASE; CELL CYCLE M PHASE; CELL CYCLE S PHASE; CELL DIFFERENTIATION; CELL FUNCTION; CELL LABELING; CELL PROLIFERATION; CELLULAR DISTRIBUTION; CONTROLLED STUDY; EPITHELIUM CELL; FLUORESCENCE; FORESTOMACH; IMMUNOHISTOCHEMISTRY; MALE; MOUSE; NONHUMAN; PRIORITY JOURNAL; PYLORUS; SKIN CELL; SKIN DEFECT;

ADRENAL GLANDS; ANIMALS; ANTIBODIES; BROMODEOXYURIDINE; CELL PROLIFERATION; GASTRIC MUCOSA; IMMUNOHISTOCHEMISTRY; INDICATORS AND REAGENTS; KI-67 ANTIGEN; MALE; MICE; MICE, INBRED C57BL; S PHASE; SKIN; STAINING AND LABELING; STOMACH; WOUND HEALING;

MAMMALIA;

EID: 80051707152     PISSN: 00221554     EISSN: None     Source Type: Journal    
DOI: 10.1369/0022155411411090     Document Type: Article

References (21)

1. Burger PC, Shibata T, Kleihues P. 1986. The use of the monoclonal antibody Ki-67 in the identification of proliferating cells: application to surgical neuropathology. Am J Surg Pathol. 10:611-617.
2. Bykorez AI, Ivashchenko YuD. 1984. Gastrointestinal stem cells and their role in carcinogenesis. Int Rev Cytol. 90:309-373.
3. Clausen OP, Kirkhus B, Schjølberg AR. 1986. Cell cycle progression kinetics of regenerating mouse epidermal cells: an in vivo study combining DNA flow cytometry, cell sorting, and [3H] dThd autoradiography. J Invest Dermatol. 86:402-405.
4. Gerdes J, Lemke H, Baisch H, Wacker HH, Schwab U, Stern H. 1984. Cell cycle analysis of a cell proliferation-associated human nuclear antigen defined by the monoclonal antibody Ki-67. J Immunol. 133:1710-1715.
5. Gerdes J, Schwab U, Lemre H, Stein H. 1982. Production of a mouse monoclonal antibody reactive with a human-nuclear antigen associated with cell proliferation. Int J Cancer. 31:13-20.
6. Gunduz N. 1985. The use of FITC-conjugated monoclonal antibodies for determination of S-phase cells with fluorescence microscopy. Cytometry. 6:597-601.
7. Hattori T, Fujita S. 1976. Tritiated thymidine autoradiographic study of cell migration and renewal in the pyloric mucosa of golden hamsters. Cell Tiss Res. 175:49-57.
8. Kajiya H, Takekoshi S, Takei M, Egashira N, Miyakoshi T, Serizawa A, Teramoto A, Osamura RY. 2009. Selection of buffer pH by the isoelectric point of the antigen for the efficient heat-induced epitope retrieval: re-appraisal for nuclear protein pathobiology. Histochem Cell Biol. 132:659-667.
9. Langer EM, Rottgers HR, Schliermann MG, Meier EM, Miltenburger HG, Schumann J, Gohde W. 1985. Cycling S-phase cells in animal and spontaneous tumours: I. Comparison of the BrdUrd and 3H-thymidine techniques and flow cytometry for the estimation of S-phase frequency. Acta Radiol Oncol. 24:545-548.
10. Lellé RJ, Heidenreich W, Stauch G, Wecke I, Gerdes J. 1987. Determination of growth fractions in benign breast disease (BBD) with monoclonal antibody Ki-67. J Cancer Res Clin Oncol. 113:73-77.
11. Lipkin M, Sherlock P, Bell B. 1964. Cell proliferation kinetics in the gastrointestinal tract of man: II. Cell renewal in stomach, ileum, colon, and rectum. Gastroenterology. 45:405-417.
12. Muskhelishvili L, Latendresse JR, Kodell RL, Henderson EB. 2003. Evaluation of cell proliferation in rat tissues with BrdU, PCNA, Ki-67(MIB-5) immunohistochemistry and in situ hybridization for histone mRNA. J Histochem Cytochem. 51:1681-1688.
13. Nakajima T, Kagawa K, Deguchi T, Kimura H, Kakusui M, Katagishi T, Mitsumoto Y, Okanoue T, Kashima K, Ashihara T. 1999. Novel formula for cell kinetics in xenograft model of hepatocellular carcinoma using histologically calculable parameters. Exp Cell Res. 246:412-420.
14. Sasaki K, Matsumura K, Tsuji T, Shinozaki F, Takahashi M. 1988. Relationship between labeling indices of Ki-67 and BrdUrd in human malignant tumors. Cancer. 62:989-993.
15. Schulte DM, Shapiro I, Reincke M, Beuschlein F. 2007. Expression and spatio-temporal distribution of differentiation and proliferation markers during mouse adrenal development. Gene Expr Patterns. 7:72-81.
16. Shi SR, Cote RJ, Yang C, Chen C, Xu HJ, Benedict WF, Taylor CR. 1996. Development of an optimal protocol for antigen retrieval: a 'test battery' approach exemplified with reference to the staining of retinoblastoma protein (pRB) in formalinfixed paraffin sections. J Pathol. 179:347-352.
17. Stevens CE, Leblond CP. 1953. Renewal of the mucous cells in the gastric mucosa of the rat. Anat Rec. 115:231-246.
18. Tsurusawa M, Aoyama M, Saeki K, Fujimoto T. 1995. Cell cycle kinetics in childhood acute leukemia studied with in vitro bromodeoxyuridine labeling, Ki67-reactivity, and flow cytometry. Leukemia. 9:1921-1925.
19. Ulrich-Lai YM, Figueiredo HF, Ostrander MM, Choi DC, Engeland WC, Herman JP. 2006. Chronic stress induces adrenal hyperplasia and hypertrophy in a subregion-specific manner. Am J Physiol Endocrinol Metab. 291:E965-E973.
20. Van Erp PE, De Mare S, Rijzewijk JJ, Van de Kerkhof PC, Bauer FW. 1989. A sequential double immunoenzymic staining procedure to obtain cell kinetic information in normal and hyperproliferative epidermis. Histochem J. 21:343-347.
21. Vries RG, Huch M, Clevers H. 2010. Stem cells and cancer of the stomach and intestine. Mol Oncol. 4:373-384.