Selection of buffer pH by the isoelectric point of the antigen for the efficient heat-induced epitope retrieval: Re-appraisal for nuclear protein pathobiology

Histochemistry and Cell Biology

Volumn 132, Issue 6, 2009, Pages 659-667

  Kajiya, Hanako ^a   Takekoshi, Susumu ^a   Takei, Mao ^b   Egashira, Noboru ^a   Miyakoshi, Takashi ^a   Serizawa, Akihito ^a   Teramoto, Akira ^b   Osamura, Robert Y ^a  

^a TOKAI UNIVERSITY   (Japan)

^b NIPPON MEDICAL SCHOOL   (Japan)

Author keywords

Heat induced epitope retrieval; Isoelectric point; Nuclear proteins; PH; Universal buffer

Indexed keywords

ANTIBODY; ANTIGEN; BUFFER; EPITOPE; FORMALDEHYDE; NUCLEAR PROTEIN; PARAFFIN; TRANSCRIPTION FACTOR;

ARTICLE; ELECTRICITY; HEATING; HUMAN; HYPOPHYSIS; IMMUNOHISTOCHEMISTRY; ISOELECTRIC POINT; PATHOLOGY; PH; PRIORITY JOURNAL;

ANTIBODIES; ANTIGENS; BUFFERS; EPITOPES; FORMALDEHYDE; HOT TEMPERATURE; HUMANS; HYDROGEN-ION CONCENTRATION; IMMUNOHISTOCHEMISTRY; ISOELECTRIC POINT; NUCLEAR PROTEINS; PARAFFIN EMBEDDING; TISSUE FIXATION;

EID: 71349083602     PISSN: 09486143     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00418-009-0635-8     Document Type: Article

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