RT real-time PCR-based quantification ofUromyces fabae in planta

FEMS Microbiology Letters

Volumn 322, Issue 2, 2011, Pages 131-137

  Voegele, Ralf T ^a   Schmid, Annette ^b  

^a UNIVERSITY OF HOHENHEIM   (Germany)

^b UNIVERSITY OF KONSTANZ   (Germany)

Author keywords

Host parasite interaction; Obligate biotrophic pathogen; Quantitative PCR

Indexed keywords

RNA;

ANALYTIC METHOD; CONTROLLED STUDY; FUNGAL DEVELOPMENT; FUNGUS; GENE EXPRESSION PROFILING; GENETIC DIFFERENCE; HAUSTORIUM; INOCULATION; LETTER; NONHUMAN; PLANT PATHOGEN INTERACTION; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ANALYSIS; STEADY STATE; UROMYCES FABAE; VICIA FABA;

BASIDIOMYCOTA; HOST-PATHOGEN INTERACTIONS; REAL-TIME POLYMERASE CHAIN REACTION; RNA, FUNGAL; TUBULIN; VICIA FABA;

FUNGI; UROMYCES VICIAE-FABAE; VICIA FABA;

EID: 80051667750     PISSN: 03781097     EISSN: 15746968     Source Type: Journal    
DOI: 10.1111/j.1574-6968.2011.02343.x     Document Type: Letter

References (26)

1. Barnes CW & Szabo LJ (2007) Detection and identification of four common rust pathogens of cereals and grasses using real-time polymerase chain reaction. Phytopathology 97: 717-727.
2. Bock CH, Parker PE, Cook AZ & Gottwald TR (2008) Visual rating and the use of image analysis for assessing different symptoms of citrus canker on grapefruit leaves. Plant Dis 92: 530-541.
3. Boyle B, Hamelin RC & Seguin A (2005) In vivo monitoring of obligate biotrophic pathogen growth by kinetic PCR. Appl Environ Microb 71: 1546-1552.
4. Chong J, Kang Z, Kim WM & Rohringer R (1992) Multinucleate condition of Puccinia striiformis in colonies isolated from infected wheat leaves with macerating enzymes. Can J Bot 70: 222-224.
5. Deising H, Jungblut PR & Mendgen K (1991) Differentiation-related proteins of the broad bean rust fungus Uromyces viciae-fabae, as revealed by high resolution two-dimensional polyacrylamide gel electrophoresis. Arch Microbiol 155: 191-198.
6. Gessner MO, Bauchrowitz MA & Escautier M (1991) Extraction and quantification of ergosterol as a measure of fungal biomass in leaf litter. Microb Ecol 22: 285-291.
7. Hahn M & Mendgen K (1992) Isolation of ConA binding haustoria from different rust fungi and comparison of their surface qualities. Protoplasma 170: 95-103.
8. Hahn M & Mendgen K (1997) Characterization of in planta-induced rust genes isolated from a haustorium-specific cDNA library. Mol Plant Microbe In 10: 427-437.
9. Heath EM (1999) Low pH RNA Isolation Reagents, Method, and Kit - United States Patent 5973137. Gentra Systems Inc., Minneapolis, MN.
10. Jakupovic M, Heintz M, Reichmann P, Mendgen K & Hahn M (2006) Microarray analysis of expressed sequence tags from haustoria of the rust fungus Uromyces fabae. Fungal Genet Biol 43: 8-19.
11. Kemen E, Kemen AC, Rafiqi M, Hempel U, Mendgen K, Hahn M & Voegele RT (2005) Identification of a protein from rust fungi transferred from haustoria into infected plant cells. Mol Plant Microbe In 18: 1130-1139.
12. Laurans F & Pilate G (1999) Histological aspects of a hypersensitive response in poplar to Melampsora larici-populina. Phytopathology 89: 233-238.
13. Manter DK, Kelsey RG & Stone JK (2001) Quantification of Phaeocryptopus gaeumannii colonization in Douglas-fir needles by ergosterol analysis. Forest Pathol 31: 229-240.
14. Martin F, Delaruelle C & Hilbert JL (1990) An improved ergosterol assay to estimate fungal biomass in ectomycorrhizas. Mycol Res 94: 1059-1064.
15. Newell SY, Arsuffi TL & Fallon RD (1988) Fundamental procedures for determining ergosterol content of decaying plant material by liquid chromatography. Appl Environ Microb 54: 1876-1879.
16. Osswald WF, Holl W & Elstner EF (1986) Ergosterol as a biochemical indicator of fungal infection in spruce and fir needles from different sources. Z Naturforsch 41: 542-546.
17. Pei MH, Ruiz C, Hunter T, Arnold GM & Bayon C (2002) Quantitative relationships between inoculum of Melampsora larici-epitea and corresponding disease on Salix. Plant Pathol 51: 443-453.
18. Sillero JC & Rubiales D (2002) Histological characterization of resistance to Uromyces viciae-fabae in faba bean. Phytopathology 92: 294-299.
19. Sohn J, Voegele RT, Mendgen K & Hahn M (2000) High level activation of vitamin B1 biosynthesis genes in haustoria of the rust fungus Uromyces fabae. Mol Plant Microbe In 13: 629-636.
20. Staples RC, Gross D, Tiburzy R, Hoch HC, Hassouna S & Werb WW (1984) Changes in DNA content of nuclei in rust uredospore germlings during the start of differentiation. Exp Mycol 8: 245-255.
21. Tisserant B, Gianinazzi-Pearson V, Gianinazzi S & Gollote A (1993) In planta histochemical staining of fungal alkaline phosphatase activity for analysis of efficient arbuscular mycorrhizal infections. Mycol Res 97: 245-250.
22. Vincelli P & Tisserat N (2008) Nucleic acid-based pathogen detection in applied plant pathology. Plant Dis 92: 660-669.
23. Voegele RT, Struck C, Hahn M & Mendgen K (2001) The role of haustoria in sugar supply during infection of broad bean by the rust fungus Uromyces fabae. P Natl Acad Sci USA 98: 8133-8138.
24. Weete JD, Abril M & Blackwell M (2010) Phylogenetic distribution of fungal sterols. PLoS One 5: e10899.
25. Winton LM, Manter DK, Stone JK & Hansen EM (2003) Comparison of biochemical, molecular, and visual methods to quantify Phaeocryptopus gaeumannii in Douglas-Fir foliage. Phytopathology 93: 121-126.
26. Wirsel SGR, Voegele RT, Bänninger R & Mendgen KW (2004) Cloning of ß-tubulin and succinate dehydrogenase genes from Uromyces fabae and establishing selection conditions for their use in transformation. Eur J Plant Pathol 110: 767-777.