DNA profiling of spermatozoa by laser capture microdissection and low volume-PCR

PLoS ONE

Volumn 6, Issue 8, 2011, Pages

  Li, Cai xia ^a   Han, Jun ping ^b   Ren, Wen yan ^b   Ji, An quan ^a   Xu, Xiu lan ^a   Hu, Lan ^a  

^a INSTITUTE OF FORENSIC SCIENCE   (China)

^b PEOPLE S PUBLIC SECURITY UNIVERSITY OF CHINA   (China)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; CELL ISOLATION; CONTROLLED STUDY; DNA FINGERPRINTING; EPITHELIUM CELL; FORENSIC IDENTIFICATION; GENOTYPE; HUMAN; HUMAN CELL; IN TUBE POLYMERASE CHAIN REACTION; LASER CAPTURE MICRODISSECTION; LOW VOLUME POLYMERASE CHAIN REACTION; POLYMERASE CHAIN REACTION; RAPE; REPRODUCIBILITY; SENSITIVITY AND SPECIFICITY; SPERMATOZOON; CELL SEPARATION; CYTOLOGY; FEMALE; GENE LOCUS; GENETICS; HAPLOIDY; LAB ON A CHIP; LABORATORY DIAGNOSIS; MALE; METABOLISM; METHODOLOGY; TANDEM REPEAT;

CELL SEPARATION; DNA FINGERPRINTING; FEMALE; GENETIC LOCI; GENOTYPE; HAPLOIDY; HUMANS; LAB-ON-A-CHIP DEVICES; LASER CAPTURE MICRODISSECTION; MALE; POLYMERASE CHAIN REACTION; RAPE; SPECIMEN HANDLING; SPERMATOZOA; TANDEM REPEAT SEQUENCES;

EID: 80051620584     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0022316     Document Type: Article

References (15)

1. McAlister C, (2011) The use of fluorescence in situ hybridisation and laser microdissection to identify and isolate male cells in an azoospermic sexual assault case. Forensic Sci Int Genet 5: 69-73.
2. Di Martino D, Giuffre G, Staiti N, Simone A, Todaro P, et al. (2004) Laser microdissection and DNA typing of cells from single hair follicles. Forensic Sci Int 146 (Suppl): S155-157.
3. Budimlija ZM, Lechpammer M, Popiolek D, Fogt F, Prinz M, et al. (2005) Forensic applications of laser capture microdissection: use in DNA-based parentage testing and platform validation. Croat Med J 46: 549-555.
4. Elliott K, Hill DS, Lambert C, Burroughes TR, Gill P, (2003) Use of laser microdissection greatly improves the recovery of DNA from sperm on microscope slides. Forensic Sci Int 137: 28-36.
5. Sanders CT, Sanchez N, Ballantyne J, Peterson DA, (2006) Laser microdissection separation of pure spermatozoa from epithelial cells for short tandem repeat analysis. J Forensic Sci 51: 748-757.
6. Anslinger K, Bayer B, Mack B, Eisenmenger W, (2007) Sex-specific fluorescent labelling of cells for laser microdissection and DNA profiling. Int J Legal Med 121: 54-56.
7. Vandewoestyne M, Van Hoofstat D, Van Nieuwerburgh F, Deforce D, (2009) Automatic detection of spermatozoa for laser capture microdissection. Int J Legal Med 123: 169-175.
8. Li CX, Qi B, Ji AQ, Xu XL, Hu L, (2009) The combination of single cell micromanipulation with LV-PCR system and its application in forensic science. Forensic Sci Int Genet S2: 516-517.
9. Miyazaki T, Hara M, Ichiki A, Yamamoto Y, Takada A, et al. (2008) An efficient novel method for analyzing STR loci from a single sperm captured by laser microdissection. Forensic Sci Int Genet S1: 437-438.
10. Schmidt U, Lutz-Bonengel S, Weisser HJ, Sanger T, Pollak S, et al. (2006) Low-volume amplification on chemically structured chips using the PowerPlex16 DNA amplification kit. Int J Legal Med 120: 42-48.
11. Lutz-Bonengel S, Sanger T, Heinrich M, Schon U, Schmidt U, (2007) Low volume amplification and sequencing of mitochondrial DNA on a chemically structured chip. Int J Legal Med 121: 68-73.
12. Gill P, Whitaker J, Flaxman C, Brown N, Buckleton J, (2000) An investigation of the rigor of interpretation rules for STRs derived from less than 100 pg of DNA. Forensic Sci Int 112: 17-40.
13. Caragine T, Mikulasovich R, Tamariz J, Bajda E, Sebestyen J, et al. (2009) Validation of testing and interpretation protocols for low template DNA samples using AmpFlSTR Identifiler. Croat Med J 50: 250-267.
14. Budowle B, Eisenberg AJ, van Daal A, (2009) Validity of low copy number typing and applications to forensic science. Croat Med J 50: 207-217.
15. Gill P, Jeffreys AJ, Werrett DJ, (1985) Forensic application of DNA 'fingerprints'. Nature 318: 577-579.