Selective and reversible thiol-pegylation, an effective approach for purification and characterization of five fully active ficin (iso)forms from Ficus carica latex

Phytochemistry

Volumn 72, Issue 14-15, 2011, Pages 1718-1731

  Azarkan, Mohamed ^a   Matagne, André ^b   Wattiez, Ruddy ^c   Bolle, Laetitia ^a   Vandenameele, Julie ^b   Baeyens Volant, Danielle ^a  

^a FREE UNIVERSITY OF BRUSSELS   (Belgium)

^b UNIVERSITY OF LIÈGE   (Belgium)

^c UNIVERSITY OF MONS   (Belgium)

Author keywords

Circular dichroism; Cysteine protease; Ficin; Ficus carica; Mass spectrometry; Thiol pegylation

Indexed keywords

AMIDASE; CYSTEINE PROTEINASE INHIBITOR; FICIN; LATEX; MACROGOL DERIVATIVE; MONOMETHOXYPOLYETHYLENE GLYCOL; THIOL DERIVATIVE; VEGETABLE PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; CHEMISTRY; CIRCULAR DICHROISM; DRUG EFFECT; ENZYMOLOGY; FICUS; ISOLATION AND PURIFICATION; MASS SPECTROMETRY; METABOLISM; PH; PROTEIN SECONDARY STRUCTURE; PROTEIN TERTIARY STRUCTURE; SEQUENCE ANALYSIS;

AMIDOHYDROLASES; AMINO ACID SEQUENCE; CIRCULAR DICHROISM; CYSTEINE PROTEINASE INHIBITORS; FICAIN; FICUS; HYDROGEN-ION CONCENTRATION; LATEX; MASS SPECTROMETRY; PLANT PROTEINS; POLYETHYLENE GLYCOLS; PROTEIN STRUCTURE, SECONDARY; PROTEIN STRUCTURE, TERTIARY; SEQUENCE ANALYSIS, PROTEIN; SULFHYDRYL COMPOUNDS;

CARICA PAPAYA; FICUS CARICA; INSECTIVORA;

EID: 80051547278     PISSN: 00319422     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.phytochem.2011.05.009     Document Type: Article

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