A method to extract intact and pure RNA from mycobacteria

Analytical Biochemistry

Volumn 417, Issue 2, 2011, Pages 286-288

  Akhtar, Shamim ^a   Sarkar, Sampa ^a   Mishra, Abhishek ^a   Sarkar, Dhiman ^a  

^a NATIONAL CHEMICAL LABORATORY   (India)

Author keywords

Dormant; Hypoxia; Mycobacteria; RNA isolation; Spheroplast; Trizol

Indexed keywords

BIOCHEMISTRY; CHEMISTRY;

DORMANT; HYPOXIA; MYCOBACTERIA; RNA ISOLATION; SPHEROPLASTS; TRIZOL;

RNA;

BACTERIAL RNA;

AEROBIC BACTERIUM; ARTICLE; BACILLUS; BACTERIAL GROWTH; BACTERIAL INFECTION; BACTERIUM ISOLATION; CONTROLLED STUDY; GENE AMPLIFICATION; HYPOXIA; IN VITRO STUDY; MACROPHAGE; MICROARRAY ANALYSIS; MYCOBACTERIUM; NONHUMAN; PRIORITY JOURNAL; QUALITATIVE ANALYSIS; QUANTITATIVE ANALYSIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA EXTRACTION;

BACILLI (CLASS); CORYNEBACTERINEAE;

EID: 80051547114     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2011.06.028     Document Type: Article

References (15)

1. B. Lewin The messenger RNA template B. Lewin, Genes IV 1990 Oxford University Press Oxford, UK 171 184
2. P.J. Brennan Structure of mycobacteria: recent developments in defining cell wall carbohydrates and proteins Rev. Infect. Dis. 11 1989 S420 S430
3. M.D. Bashyam, and A.K. Tyagi An efficient and high yielding method for isolation of RNA from mycobacteria Biotechniques 17 1994 834 836
4. S. Kinger, A. Verma, and J.S. Tyagi A method for the isolation of pure intact RNA from mycobacteria Biotechniques 14 1993 724 725 (Pubitemid 23145482)
5. A.L. Cheung, K.J. Eberhardt, and V.A. Fischetti A method to isolate RNA from Gram-positive bacteria and mycobacteria Anal. Biochem. 222 1994 511 514 (Pubitemid 24338880)
6. L.C. Kikuta-Oshima, F.D. Quinn, W.R. Butler, T.M. Shinnick, and C.H. King Isolation of RNA from Mycobacterium tuberculosis using a nitrogen decompression chamber Biotechniques 18 1995 987 990
7. J.A. Mangan, K.M. Sole, D.A. Mitchison, and P.D. Butcher An effective method of RNA extraction from bacteria refractory to disruption, including mycobacteria Nucleic Acids Res. 25 1997 675 676 (Pubitemid 27298254)
8. A. Khan, and D. Sarkar Identification of a respiratory-type nitrate reductase and its role for survival of Mycobacterium smegmatis in Wayne model Microb. Pathog. 41 2006 90 95 (Pubitemid 44223946)
9. L.G. Wayne, and L.G. Hayes An in vitro model for sequential study of shiftdown of Mycobacterium tuberculosis through two stages of non-replicating persistence Infect. Immun. 64 1996 2062 2069 (Pubitemid 26165667)
10. P. Chomczynski, and N. Sacchi Single-step method of RNA isolation by guanidinium thiocyanate-phenol chloroform extraction Anal. Biochem. 162 1987 156 159 (Pubitemid 17064313)
11. U.E. Schaible, S. Sturgill-Koszycki, P.H. Schlesinger, and D.G. Russell Cytokine activation leads to acidification and increases maturation of Mycobacterium avium-containing phagosomes in murine macrophages J. Immunol. 160 1998 1290 1296 (Pubitemid 28093427)
12. G. Dietrich, U.E. Schaible, and K.D. Diehl Isolation of RNA from mycobacteria grown under in vitro and in vivo conditions FEMS Microbiol. Lett. 186 2000 177 180 (Pubitemid 30232738)
13. V. Nygaard, A. Loland, and E. Hovig Effects of mRNA amplification on gene expression ratios in cDNA experiments estimated by analysis of variance BMC Genomics 4 2003 11
14. I.M. Monhan, J.A. Mangan, and P.D. Butcher Extraction of RNA from intracellular Mycobacterium tuberculosis: methods, considerations, and applications T. Parish, N.G. Stoker, Mycobacterium tuberculosis Protocols 2001 Humana Totowa, NJ 31 42
15. N. Rastogi, and H.L. David Ultrastructural and chemical studies on wall-deficient forms, spheroplasts, and membrane vesicles from Mycobacterium aurum J. Gen. Microbiol. 124 1981 71 79 (Pubitemid 11093620)