Identification of deletion mutant respiratory syncytial virus strains lacking most of the G protein in immunocompromised children with pneumonia in South Africa

Journal of Virology

Volumn 85, Issue 16, 2011, Pages 8453-8457

  Venter, Marietjie ^a,b   van Niekerk, Stephanie ^a   Rakgantso, Andronica ^a,b   Bent, Nicola ^a  

^a UNIVERSITY OF PRETORIA   (South Africa)

^b NATIONAL HEALTH LABORATORY SERVICE   (South Africa)

Author keywords

[No Author keywords available]

Indexed keywords

GUANINE NUCLEOTIDE BINDING PROTEIN;

AMINO ACID SEQUENCE; AMPLICON; ARTICLE; CHILD; CONTROLLED STUDY; DELETION MUTANT; DISEASE ASSOCIATION; GENOTYPE; HOSPITALIZED CHILD; HUMAN; HUMAN IMMUNODEFICIENCY VIRUS INFECTION; IMMUNOCOMPROMISED PATIENT; LOWER RESPIRATORY TRACT INFECTION; MAJOR CLINICAL STUDY; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DOMAIN; RESPIRATORY SYNCYTIAL PNEUMOVIRUS; SEQUENCE ANALYSIS; SOUTH AFRICA; VIRUS IDENTIFICATION; VIRUS MUTANT; VIRUS PNEUMONIA; VIRUS STRAIN;

RESPIRATORY SYNCYTIAL VIRUS; RICE STRIPE VIRUS;

AMINO ACID SEQUENCE; CD4-POSITIVE T-LYMPHOCYTES; CHILD; CHILD, PRESCHOOL; HUMANS; IMMUNOCOMPROMISED HOST; INFANT; MOLECULAR SEQUENCE DATA; PNEUMONIA, VIRAL; POLYMERASE CHAIN REACTION; RESPIRATORY SYNCYTIAL VIRUS INFECTIONS; RESPIRATORY SYNCYTIAL VIRUS, HUMAN; RNA, VIRAL; SEQUENCE ALIGNMENT; SEQUENCE DELETION; SOUTH AFRICA; VIRAL FUSION PROTEINS;

EID: 79961178699     PISSN: 0022538X     EISSN: 10985514     Source Type: Journal    
DOI: 10.1128/JVI.00674-11     Document Type: Article

References (26)

1. Anderson, L. J., et al. 1985. Antigenic characterization of respiratory syncytial virus strains with monoclonal antibodies. J. Infect. Dis. 151:626-633.
2. Collins, P. L., and B. R. Murphy. 2002. Respiratory syncytial virus: reverse genetics and vaccine strategies. Virology 296:204-211.
3. Feldman, S. A., S. Audet, and J. A. Beeler. 2000. The fusion glycoprotein of human respiratory syncytial virus facilitates virus attachment and infectivity via an interaction with cellular heparan sulfate. J. Virol. 74:6442-6447.
4. Hall, T. A. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp. Ser. 41:95-98.
5. Johnson, P. R., M. K. Spriggs, R. A. Olmsted, and P. L. Collins. 1987. The G glycoprotein of human respiratory syncytial viruses of subgroups A and B: extensive sequence divergence between antigenically related proteins. Proc. Natl. Acad. Sci. U. S. A. 84:5625-5629.
6. Karron, R. A., et al. 1997. Respiratory syncytial virus (RSV) SH and G proteins are not essential for viral replication in vitro: clinical evaluation and molecular characterization of a cold-passaged, attenuated RSV subgroup B mutant. Proc. Natl. Acad. Sci. U. S. A. 94:13961-13966.
7. Lassauniere, R., T. Kresfelder, and M. Venter. 2010. A novel multiplex real-time RT-PCR assay with FRET hybridization probes for the detection and quantitation of 13 respiratory viruses. J. Virol. Methods 165: 254-260.
8. Lazar, I., A. Canaan, C. Weibel, and J. S. Kahn. 2006. Novel mutations in the respiratory syncytial virus G gene identified in viral isolates from a girl with severe combined immune deficiency treated with intravenous immune globulin. J. Clin. Virol. 37:168-173.
9. Madhi, S. A., M. Venter, A. Madhi, M. K. Petersen, and K. P. Klugman. 2001. Differing manifestations of respiratory syncytial virus-associated severe lower respiratory tract infections in human immunodeficiency virus type 1-infected and uninfected children. Pediatr. Infect. Dis. J. 20: 164-170.
10. Martinez, I., et al. 1999. Evolutionary pattern of the G glycoprotein of human respiratory syncytial viruses from antigenic group B: the use of alternative termination codons and lineage diversification. J. Gen. Virol. 80(Pt. 1):125-130.
11. Melero, J. A., B. Garcia-Barreno, I. Martinez, C. R. Pringle, and P. A. Cane. 1997. Antigenic structure, evolution and immunobiology of human respiratory syncytial virus attachment (G) protein. J. Gen. Virol. 78(Pt. 10):2411-2418.
12. National Department of Health South Africa. 2010. Guidelines for the management of HIV in children. National Department of Health South Africa, Pretoria, South Africa. www.hiv911.org.za/wp-content/uploads/2010/04/2010 -Paediatric-Guidelines.pdf.
13. Peret, T. C., et al. 2000. Circulation patterns of group A and B human respiratory syncytial virus genotypes in 5 communities in North America. J. Infect. Dis. 181:1891-1896.
14. Peret, T. C., C. B. Hall, K. C. Schnabel, J. A. Golub, and L. J. Anderson. 1998. Circulation patterns of genetically distinct group A and B strains of human respiratory syncytial virus in a community. J. Gen. Virol. 79:2221-2229.
15. Sullender, W. M., L. Sun, and L. J. Anderson. 1993. Analysis of respiratory syncytial virus genetic variability with amplified cDNAs. J. Clin. Microbiol. 31:1224-1231.
16. Techaarpornkul, S., P. L. Collins, and M. E. Peeples. 2002. Respiratory syncytial virus with the fusion protein as its only viral glycoprotein is less dependent on cellular glycosaminoglycans for attachment than complete virus. Virology 294:296-304.
17. Teng, M. N., and P. L. Collins. 2002. The central conserved cystine noose of the attachment G protein of human respiratory syncytial virus is not required for efficient viral infection in vitro or in vivo. J. Virol. 76:6164-6171.
18. Teng, M. N., S. S. Whitehead, and P. L. Collins. 2001. Contribution of the respiratory syncytial virus G glycoprotein and its secreted and membranebound forms to virus replication in vitro and in vivo. Virology 289:283-296.
19. Thompson, J. D., T. J. Gibson, F. Plewniak, F. Jeanmougin, and D. G. Higgins. 1997. The CLUSTAL_X Windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 25:4876-4882.
20. Trento, A., et al. 2010. Ten years of global evolution of the human respiratory syncytial virus BA genotype with a 60-nucleotide duplication in the G protein gene. J. Virol. 84:7500-7512.
21. Trento, A., et al. 2003. Major changes in the G protein of human respiratory syncytial virus isolates introduced by a duplication of 60 nucleotides. J. Gen. Virol. 84:3115-3120.
22. Trento, A., et al. 2006. Natural history of human respiratory syncytial virus inferred from phylogenetic analysis of the attachment (G) glycoprotein with a 60-nucleotide duplication. J. Virol. 80:975-984.
23. Venter, M., M. Collinson, and B. D. Schoub. 2002. Molecular epidemiological analysis of community circulating respiratory syncytial virus in rural South Africa: comparison of viruses and genotypes responsible for different disease manifestations. J. Med. Virol. 68:452-461.
24. Venter, M., S. A. Madhi, C. T. Tiemessen, and B. D. Schoub. 2001. Genetic diversity and molecular epidemiology of respiratory syncytial virus over four consecutive seasons in South Africa: identification of new subgroup A and B genotypes. J. Gen. Virol. 82:2117-2124.
25. Visser, A., S. Delport, and M. Venter. 2008. Molecular epidemiological analysis of a nosocomial outbreak of respiratory syncytial virus associated pneumonia in a kangaroo mother care unit in South Africa. J. Med. Virol. 80:724-732.
26. Zlateva, K., P. Lemey, E. Moes, A. Vandamme, and M. V. Ranst. 2005. Genetic variability and molecular evolution of the human respiratory syncytial virus subgroup B attachment G protein. J. Virol. 79:9157-9167.