Rapid isolation of genomic DNA from E. coli XL1 Blue strain approaching bare magnetic nanoparticles

Current Science

Volumn 101, Issue 2, 2011, Pages 210-214

  Bandyopadhyay, A ^a   Chatterjee, S ^a   Sarkar, K ^a  

^a UNIVERSITY OF KALYANI   (India)

Author keywords

Bacterial genomic DNA; Escherichia coli; Isolation and purification; Magnetic nanoparticles

Indexed keywords

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 79961149506     PISSN: 00113891     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (25)

1. Kirby, K. S., A new method for the isolation of deoxyribonucleic acids: evidence on the nature of bonds between deoxyribonucleic acid protein. Biochem. J., 1957, 66, 495-504.
2. Kirby, K. S., Fox-Carter, E. and Guest, M., Isolation of deoxyribonucleic acid and ribosomal ribonucleic acid from bacteria. Biochem. J., 1967, 104, 258-262.
3. Sambrook, J., Fritsch, E. F. and Maniatis, T., Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1989, 2nd edn.
4. Robinson, P. J., Dunnill, P. and Lilly, M. D., Properties of magnetite supports in relation to immobilized enzyme reactors. Biotechnol. Bioeng., 1973, 15, 603-606.
5. Magnani, M., Galluzzi, L. and Bruce, I. J., The use of magnetic nanoparticles in the development of new molecular detection systems. J. Nanosci. Nanotechnol., 2006, 6, 2302-2311.
6. Amagliani, G., Omiccioli, E., Campo, A., Bruce, I. J., Brandi, G. and Magnani, M., Development of a magnetic capture hybridization-PCR assay for Listeria monocytogenes direct detection in milk samples. J. Appl. Microbiol., 2006, 100, 375-383.
7. Galluzzi, L., Bertozzini, E., del Campo, A., Penna, A., Bruce, I. J. and Magnani, M., Capture probe conjugated to paramagnetic nanoparticles for purification of Alexandrium species (Dinophyceae) DNA from environmental samples. J. Appl. Microbiol., 2006, 101, 36-43.
8. Sen, T., Sebastianelli, A. and Bruce, I. J., Mesoporous silica magnetite nanocomposite: fabrication and applications in magnetic bioseparations. J. Am. Chem. Soc., 2006, 128, 7130-7131.
9. Mehta, R. V., Upadhyay, R. V., Charles, S. W. and Ramchand, C. N., Direct binding of protein to magnetic particles. Biotechnol. Tech., 1997, 11, 493-496.
10. 3 nanoparticles dispersed over a silica matrix. J. Phys. Chem. B, 1998, 102(40), 7721-7726.
11. Benito, J. M., Lovrich, G. A., Siñeriz, F. and Abate, C. M., Isolation and molecular characterization of seawater bacteria. In Environmental Microbiology: Methods and Protocols (eds Spencer, J. F. T. and Spencer, A. L. R.), First Indian reprint, Humana Press, New Delhi, 2008, pp. 3-9.
12. Lowe, M., Spiro, A., Summers, A. O. and Wireman, J., Multiplexed identification and quantification of analyte DNAs in environmental samples using microspheres and flow cytometry. In Environmental Microbiology: Methods and Protocols (eds Spencer, J. F. T. and Spencer, A. L. R.), First Indian reprint, Humana Press, New Delhi, 2008, pp. 51-74.
13. Saiyed, Z. M., Telang, S. D. and Ramchand, C. N., Application of magnetic techniques in the field of drug discovery and biomedicine. Biomagn. Res. Technol., 2003, 1, e2.
14. Safarik, I. and Safarikova, M., Magnetic nanoparticles and biosciences. Monatsh. Chem., 2002, 133, 737-759.
15. Davies, M. J., Smethurst, D. E., Howard, K., Todd, M., Higgins, L. M. and Bruce, I. J., Improved manufacture and application of an agarose magnetizable solid-phase support. Appl. Biochem. Biotechnol., 1997, 68, 95-112.
16. Prodelalova, J., Rittich, B., Spanova, A., Petrova, K. and Benes, M. J., Isolation of genomic DNA using magnetic cobalt ferrite and silica particles. J. Chromatogr. A, 2004, 1056, 43-48.
17. Xie, X., Zhang, X., Zhang, H., Chen, D. and Fei, W., Preparation and application of surface-coated superparamagnetic nanobeads in the isolation of genomic DNA. J. Magn. Magn. Mater., 2004, 277, 16-23.
18. Nagy, M. et al., Optimization and validation of a fully automated silica-coated magnetic beads purification technology in forensics. Forensic Sci. Int., 2005, 152, 13-22.
19. Chiang, C. L., Sung, C. S., Wu, T. F., Chen, C. Y. and Hsu, C. Y., Application of superparamagnetic nanoparticles in purification of plasmid DNA from bacterial cells. J. Chromatogr. B Analyt. Technol. B, 2005, 822, 54-60.
20. Saiyed, Z. M., Bochiwal, C., Gorasia, H., Telang, S. D. and Ramchand, C. N., Application of magnetic particles (Fe3O4) for isolation of genomic DNA from mammalian cells. Anal. Biochem., 2006, 356, 306-308.
21. Saiyed, Z. M., Parasramka, M., Telang, S. D. and Ramchand, C. N., Extraction of DNA from agarose gel using magnetic nanoparticles (magnetite or Fe3O4). Anal. Biochem., 2007, 363, 288-290.
22. Hawkins, T. L., O'Connor-Morin, T., Roy, A. and Santillan, C., DNA purification and isolation using a solid-phase. Nucleic Acids Res., 1994, 22, 4543-4544.
23. Yeates, C., Gillings, M. R., Davison, A. D., Altavilla, N. and Veal, D. A., Methods for microbial DNA extraction from soil for PCR amplification. Biol. Proc. Online, 1998, 1, 40-47.
24. Davies, M. J., Taylor, J. I., Sachsinger, N. and Bruce, I. J., Isolation of plasmid DNA using magnetite as a solid phase adsorbent. Anal. Biochem., 1998, 262, 92-94.
25. Taylor, J. I., Hurst, C. D., Davies, M. J., Sachsinger, N. and Bruce, I. J., Application of magnetite and silica-magnetite composites to the isolation of genomic DNA. J. Chromatogr. A., 2000, 890, 159-166.